IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0055817.html
   My bibliography  Save this article

Alterations in the Colonic Microbiota in Response to Osmotic Diarrhea

Author

Listed:
  • Gregor Gorkiewicz
  • Gerhard G Thallinger
  • Slave Trajanoski
  • Stefan Lackner
  • Gernot Stocker
  • Thomas Hinterleitner
  • Christian Gülly
  • Christoph Högenauer

Abstract

Background & Aims: Diseases of the human gastrointestinal (GI) tract are often accompanied by diarrhea with profound alterations in the GI microbiota termed dysbiosis. Whether dysbiosis is due to the disease itself or to the accompanying diarrhea remains elusive. With this study we characterized the net effects of osmotic diarrhea on the composition of the GI microbiota in the absence of disease. Methods: We induced osmotic diarrhea in four healthy adults by oral administration of polyethylene glycol 4000 (PEG). Stool as well as mucosa specimens were collected before, during and after diarrhea and 16S rDNA-based microbial community profiling was used to assess the microbial community structure. Results: Stool and mucosal microbiotas were strikingly different, with Firmicutes dominating the mucosa and Bacteroidetes the stools. Osmotic diarrhea decreased phylotype richness and showed a strong tendency to equalize the otherwise individualized microbiotas on the mucosa. Moreover, diarrhea led to significant relative shifts in the phyla Bacteroidetes and Firmicutes and to a relative increase in the abundance of Proteobacteria on the mucosa, a phenomenon also noted in several inflammatory and diarrheal GI diseases. Conclusions: Changes in microbial community structure induced by osmotic diarrhea are profound and show similarities to changes observed in other GI diseases including IBD. These effects so must be considered when specimens from diarrheal diseases (i.e. obtained by stratification of samples according to diarrheal status) or conditions wherein bowel preparations like PEG (i.e. specimens obtained during endoscopy) are used.

Suggested Citation

  • Gregor Gorkiewicz & Gerhard G Thallinger & Slave Trajanoski & Stefan Lackner & Gernot Stocker & Thomas Hinterleitner & Christian Gülly & Christoph Högenauer, 2013. "Alterations in the Colonic Microbiota in Response to Osmotic Diarrhea," PLOS ONE, Public Library of Science, vol. 8(2), pages 1-17, February.
    • Handle: RePEc:plo:pone00:0055817
    DOI: 10.1371/journal.pone.0055817
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0055817
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0055817&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0055817?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. James Robert White & Niranjan Nagarajan & Mihai Pop, 2009. "Statistical Methods for Detecting Differentially Abundant Features in Clinical Metagenomic Samples," PLOS Computational Biology, Public Library of Science, vol. 5(4), pages 1-11, April.
    2. Charles K Lee & Craig W Herbold & Shawn W Polson & K Eric Wommack & Shannon J Williamson & Ian R McDonald & S Craig Cary, 2012. "Groundtruthing Next-Gen Sequencing for Microbial Ecology–Biases and Errors in Community Structure Estimates from PCR Amplicon Pyrosequencing," PLOS ONE, Public Library of Science, vol. 7(9), pages 1-12, September.
    3. Matthew G Bakker & Zheng J Tu & James M Bradeen & Linda L Kinkel, 2012. "Implications of Pyrosequencing Error Correction for Biological Data Interpretation," PLOS ONE, Public Library of Science, vol. 7(8), pages 1-9, August.
    4. Peter J. Turnbaugh & Ruth E. Ley & Micah Hamady & Claire M. Fraser-Liggett & Rob Knight & Jeffrey I. Gordon, 2007. "The Human Microbiome Project," Nature, Nature, vol. 449(7164), pages 804-810, October.
    5. Theodore A. Kennedy & Shahid Naeem & Katherine M. Howe & Johannes M. H. Knops & David Tilman & Peter Reich, 2002. "Biodiversity as a barrier to ecological invasion," Nature, Nature, vol. 417(6889), pages 636-638, June.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Chongqing Wen & Liyou Wu & Yujia Qin & Joy D Van Nostrand & Daliang Ning & Bo Sun & Kai Xue & Feifei Liu & Ye Deng & Yuting Liang & Jizhong Zhou, 2017. "Evaluation of the reproducibility of amplicon sequencing with Illumina MiSeq platform," PLOS ONE, Public Library of Science, vol. 12(4), pages 1-20, April.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Shilan Li & Jianxin Shi & Paul Albert & Hong-Bin Fang, 2022. "Dependence Structure Analysis and Its Application in Human Microbiome," Mathematics, MDPI, vol. 11(1), pages 1-14, December.
    2. Zhenqiu Liu & Dechang Chen & Li Sheng & Amy Y Liu, 2013. "Class Prediction and Feature Selection with Linear Optimization for Metagenomic Count Data," PLOS ONE, Public Library of Science, vol. 8(3), pages 1-7, March.
    3. Kumar P Mainali & Sharon Bewick & Peter Thielen & Thomas Mehoke & Florian P Breitwieser & Shishir Paudel & Arjun Adhikari & Joshua Wolfe & Eric V Slud & David Karig & William F Fagan, 2017. "Statistical analysis of co-occurrence patterns in microbial presence-absence datasets," PLOS ONE, Public Library of Science, vol. 12(11), pages 1-21, November.
    4. Amirhossein Shamsaddini & Kimia Dadkhah & Patrick M Gillevet, 2020. "BiomMiner: An advanced exploratory microbiome analysis and visualization pipeline," PLOS ONE, Public Library of Science, vol. 15(6), pages 1-13, June.
    5. Patricio S La Rosa & Berkley Shands & Elena Deych & Yanjiao Zhou & Erica Sodergren & George Weinstock & William D Shannon, 2012. "Statistical Object Data Analysis of Taxonomic Trees from Human Microbiome Data," PLOS ONE, Public Library of Science, vol. 7(11), pages 1-12, November.
    6. Joseph George Ray & Prasanthkumar Santhakumaran & Santhoshkumar Kookal, 2021. "Phytoplankton communities of eutrophic freshwater bodies (Kerala, India) in relation to the physicochemical water quality parameters," Environment, Development and Sustainability: A Multidisciplinary Approach to the Theory and Practice of Sustainable Development, Springer, vol. 23(1), pages 259-290, January.
    7. Pengfei Song & Wen Qin & YanGan Huang & Lei Wang & Zhenyuan Cai & Tongzuo Zhang, 2020. "Grazing Management Influences Gut Microbial Diversity of Livestock in the Same Area," Sustainability, MDPI, vol. 12(10), pages 1-12, May.
    8. Zu-Xing Wang & Zheng-Sheng He & Wei-Ming He, 2021. "Nighttime climate warming enhances inhibitory effects of atmospheric nitrogen deposition on the success of invasive Solidago canadensis," Climatic Change, Springer, vol. 167(1), pages 1-15, July.
    9. Stephen A Stanhope, 2010. "Occupancy Modeling, Maximum Contig Size Probabilities and Designing Metagenomics Experiments," PLOS ONE, Public Library of Science, vol. 5(7), pages 1-10, July.
    10. Daphna Rothschild & Erez Dekel & Jean Hausser & Anat Bren & Guy Aidelberg & Pablo Szekely & Uri Alon, 2014. "Linear Superposition and Prediction of Bacterial Promoter Activity Dynamics in Complex Conditions," PLOS Computational Biology, Public Library of Science, vol. 10(5), pages 1-9, May.
    11. Allison G. White & George S. Watts & Zhenqiang Lu & Maria M. Meza-Montenegro & Eric A. Lutz & Philip Harber & Jefferey L. Burgess, 2014. "Environmental Arsenic Exposure and Microbiota in Induced Sputum," IJERPH, MDPI, vol. 11(2), pages 1-15, February.
    12. Jae-Chang Cho, 2021. "Human microbiome privacy risks associated with summary statistics," PLOS ONE, Public Library of Science, vol. 16(4), pages 1-11, April.
    13. Pirjo Wacklin & Harri Mäkivuokko & Noora Alakulppi & Janne Nikkilä & Heli Tenkanen & Jarkko Räbinä & Jukka Partanen & Kari Aranko & Jaana Mättö, 2011. "Secretor Genotype (FUT2 gene) Is Strongly Associated with the Composition of Bifidobacteria in the Human Intestine," PLOS ONE, Public Library of Science, vol. 6(5), pages 1-10, May.
    14. Yee Sang Wong & Nicholas John Osborne, 2022. "Biodiversity Effects on Human Mental Health via Microbiota Alterations," IJERPH, MDPI, vol. 19(19), pages 1-13, September.
    15. Weiyue Ji & Handuo Shi & Haoqian Zhang & Rui Sun & Jingyi Xi & Dingqiao Wen & Jingchen Feng & Yiwei Chen & Xiao Qin & Yanrong Ma & Wenhan Luo & Linna Deng & Hanchi Lin & Ruofan Yu & Qi Ouyang, 2013. "A Formalized Design Process for Bacterial Consortia That Perform Logic Computing," PLOS ONE, Public Library of Science, vol. 8(2), pages 1-9, February.
    16. Disha Tandon & Mohammed Monzoorul Haque & Sharmila S Mande, 2016. "Inferring Intra-Community Microbial Interaction Patterns from Metagenomic Datasets Using Associative Rule Mining Techniques," PLOS ONE, Public Library of Science, vol. 11(4), pages 1-16, April.
    17. Eric Z. Chen & Frederic D. Bushman & Hongzhe Li, 2017. "A Model-Based Approach for Species Abundance Quantification Based on Shotgun Metagenomic Data," Statistics in Biosciences, Springer;International Chinese Statistical Association, vol. 9(1), pages 13-27, June.
    18. Kevin M. Mendez & Stacey N. Reinke & Rachel S. Kelly & Qingwen Chen & Mark Su & Michael McGeachie & Scott Weiss & David I. Broadhurst & Jessica A. Lasky-Su, 2025. "A roadmap to precision medicine through post-genomic electronic medical records," Nature Communications, Nature, vol. 16(1), pages 1-12, December.
    19. Serra W. Buchanan & Megan Baskerville & Maren Oelbermann & Andrew M. Gordon & Naresh V. Thevathasan & Marney E. Isaac, 2020. "Plant Diversity and Agroecosystem Function in Riparian Agroforests: Providing Ecosystem Services and Land-Use Transition," Sustainability, MDPI, vol. 12(2), pages 1-12, January.
    20. Kaixuan Pan & Merijn Moens & Leon Marshall & Ellen Cieraad & Geert R de Snoo & Koos Biesmeijer, 2021. "Importance of natural land cover for plant species’ conservation: A nationwide study in The Netherlands," PLOS ONE, Public Library of Science, vol. 16(11), pages 1-25, November.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0055817. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.