IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0021516.html
   My bibliography  Save this article

Three-Dimensional X-ray Observation of Atmospheric Biological Samples by Linear-Array Scanning-Electron Generation X-ray Microscope System

Author

Listed:
  • Toshihiko Ogura

Abstract

Recently, we developed a soft X-ray microscope called the scanning-electron generation X-ray microscope (SGXM), which consists of a simple X-ray detection system that detects X-rays emitted from the interaction between a scanning electron beam (EB) and the thin film of the sample mount. We present herein a three-dimensional (3D) X-ray detection system that is based on the SGXM technology and designed for studying atmospheric biological samples. This 3D X-ray detection system contains a linear X-ray photodiode (PD) array. The specimens are placed under a CuZn-coated Si3N4 thin film, which is attached to an atmospheric sample holder. Multiple tilt X-ray images of the samples are detected simultaneously by the linear array of X-ray PDs, and the 3D structure is calculated by a new 3D reconstruction method that uses a simulated-annealing algorithm. The resulting 3D models clearly reveal the inner structure of the bacterium. In addition, the proposed method can easily be used for diverse samples in a broad range of scientific fields.

Suggested Citation

  • Toshihiko Ogura, 2011. "Three-Dimensional X-ray Observation of Atmospheric Biological Samples by Linear-Array Scanning-Electron Generation X-ray Microscope System," PLOS ONE, Public Library of Science, vol. 6(6), pages 1-9, June.
    • Handle: RePEc:plo:pone00:0021516
    DOI: 10.1371/journal.pone.0021516
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0021516
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0021516&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0021516?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Andrej Sali & Robert Glaeser & Thomas Earnest & Wolfgang Baumeister, 2003. "From words to literature in structural proteomics," Nature, Nature, vol. 422(6928), pages 216-225, March.
    2. Emiel de Smit & Ingmar Swart & J. Fredrik Creemer & Gerard H. Hoveling & Mary K. Gilles & Tolek Tyliszczak & Patricia J. Kooyman & Henny W. Zandbergen & Cynthia Morin & Bert M. Weckhuysen & Frank M. F, 2008. "Nanoscale chemical imaging of a working catalyst by scanning transmission X-ray microscopy," Nature, Nature, vol. 456(7219), pages 222-225, November.
    3. Jannik C. Meyer & C. O. Girit & M. F. Crommie & A. Zettl, 2008. "Imaging and dynamics of light atoms and molecules on graphene," Nature, Nature, vol. 454(7202), pages 319-322, July.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Toshihiko Ogura, 2019. "Direct observation of unstained biological samples in water using newly developed impedance scanning electron microscopy," PLOS ONE, Public Library of Science, vol. 14(8), pages 1-17, August.
    2. Toshihiko Ogura, 2012. "High-Contrast Observation of Unstained Proteins and Viruses by Scanning Electron Microscopy," PLOS ONE, Public Library of Science, vol. 7(10), pages 1-7, October.
    3. Zachary C. Drake & Justin T. Seffernick & Steffen Lindert, 2022. "Protein complex prediction using Rosetta, AlphaFold, and mass spectrometry covalent labeling," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    4. Lukas Grote & Martin Seyrich & Ralph Döhrmann & Sani Y. Harouna-Mayer & Federica Mancini & Emilis Kaziukenas & Irene Fernandez-Cuesta & Cecilia A. Zito & Olga Vasylieva & Felix Wittwer & Michal Odstrč, 2022. "Imaging Cu2O nanocube hollowing in solution by quantitative in situ X-ray ptychography," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0021516. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.