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Site and Strain-Specific Variation in Gut Microbiota Profiles and Metabolism in Experimental Mice

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  • Melissa K Friswell
  • Helen Gika
  • Ian J Stratford
  • Georgios Theodoridis
  • Brian Telfer
  • Ian D Wilson
  • Andrew J McBain

Abstract

Background: The gastrointestinal tract microbiota (GTM) of mammals is a complex microbial consortium, the composition and activities of which influences mucosal development, immunity, nutrition and drug metabolism. It remains unclear whether the composition of the dominant GTM is conserved within animals of the same strain and whether stable GTMs are selected for by host-specific factors or dictated by environmental variables. Methodology/Principal Findings: The GTM composition of six highly inbred, genetically distinct strains of mouse (C3H, C57, GFEC, CD1, CBA nu/nu and SCID) was profiled using eubacterial –specific PCR-DGGE and quantitative PCR of feces. Animals exhibited strain-specific fecal eubacterial profiles that were highly stable (c. >95% concordance over 26 months for C57). Analyses of mice that had been relocated before and after maturity indicated marked, reproducible changes in fecal consortia and that occurred only in young animals. Implantation of a female BDF1 mouse with genetically distinct (C57 and Agoutie) embryos produced highly similar GTM profiles (c. 95% concordance) between mother and offspring, regardless of offspring strain, which was also reflected in urinary metabolite profiles. Marked institution-specific GTM profiles were apparent in C3H mice raised in two different research institutions. Conclusion/Significance: Strain-specific data were suggestive of genetic determination of the composition and activities of intestinal symbiotic consortia. However, relocation studies and uterine implantation demonstrated the dominance of environmental influences on the GTM. This was manifested in large variations between isogenic adult mice reared in different research institutions.

Suggested Citation

  • Melissa K Friswell & Helen Gika & Ian J Stratford & Georgios Theodoridis & Brian Telfer & Ian D Wilson & Andrew J McBain, 2010. "Site and Strain-Specific Variation in Gut Microbiota Profiles and Metabolism in Experimental Mice," PLOS ONE, Public Library of Science, vol. 5(1), pages 1-9, January.
    • Handle: RePEc:plo:pone00:0008584
    DOI: 10.1371/journal.pone.0008584
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    References listed on IDEAS

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    1. Aaron C Ericsson & J Wade Davis & William Spollen & Nathan Bivens & Scott Givan & Catherine E Hagan & Mark McIntosh & Craig L Franklin, 2015. "Effects of Vendor and Genetic Background on the Composition of the Fecal Microbiota of Inbred Mice," PLOS ONE, Public Library of Science, vol. 10(2), pages 1-19, February.
    2. Camilla Hartmann Friis Hansen & Dennis Sandris Nielsen & Miloslav Kverka & Zuzana Zakostelska & Klara Klimesova & Tomas Hudcovic & Helena Tlaskalova-Hogenova & Axel Kornerup Hansen, 2012. "Patterns of Early Gut Colonization Shape Future Immune Responses of the Host," PLOS ONE, Public Library of Science, vol. 7(3), pages 1-7, March.
    3. Willie A Bidot & Aaron C Ericsson & Craig L Franklin, 2018. "Effects of water decontamination methods and bedding material on the gut microbiota," PLOS ONE, Public Library of Science, vol. 13(10), pages 1-16, October.
    4. Hannah Lees & Jonathan Swann & Simon M Poucher & Jeremy K Nicholson & Elaine Holmes & Ian D Wilson & Julian R Marchesi, 2014. "Age and Microenvironment Outweigh Genetic Influence on the Zucker Rat Microbiome," PLOS ONE, Public Library of Science, vol. 9(9), pages 1-11, September.

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