IDEAS home Printed from https://ideas.repec.org/a/nat/nature/v391y1998i6669d10.1038_35888.html
   My bibliography  Save this article

Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans

Author

Listed:
  • Andrew Fire

    (Carnegie Institution of Washington)

  • SiQun Xu

    (Carnegie Institution of Washington)

  • Mary K. Montgomery

    (Carnegie Institution of Washington)

  • Steven A. Kostas

    (Carnegie Institution of Washington
    Biology Graduate Program, Johns Hopkins University)

  • Samuel E. Driver

    (Program in Molecular Medicine, University of Massachusetts Cancer Center)

  • Craig C. Mello

    (Program in Molecular Medicine, University of Massachusetts Cancer Center)

Abstract

Experimental introduction of RNA into cells can be used in certain biological systems to interfere with the function of an endogenous gene1,2. Such effects have been proposed to result from a simple antisense mechanism that depends on hybridization between the injected RNA and endogenous messenger RNA transcripts. RNA interference has been used in the nematode Caenorhabditis elegans to manipulate gene expression3,4. Here we investigate the requirements for structure and delivery of the interfering RNA. To our surprise, we found that double-stranded RNA was substantially more effective at producing interference than was either strand individually. After injection into adult animals, purified single strands had at most a modest effect, whereas double-stranded mixtures caused potent and specific interference. The effects of this interference were evident in both the injected animals and their progeny. Only a few molecules of injected double-stranded RNA were required per affected cell, arguing against stochiometric interference with endogenous mRNA and suggesting that there could be a catalytic or amplification component in the interference process.

Suggested Citation

  • Andrew Fire & SiQun Xu & Mary K. Montgomery & Steven A. Kostas & Samuel E. Driver & Craig C. Mello, 1998. "Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans," Nature, Nature, vol. 391(6669), pages 806-811, February.
    • Handle: RePEc:nat:nature:v:391:y:1998:i:6669:d:10.1038_35888
    DOI: 10.1038/35888
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/35888
    File Function: Abstract
    Download Restriction: Access to the full text of the articles in this series is restricted.
    File URL: https://libkey.io/10.1038/35888?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Petersen, Alexander M. & Rotolo, Daniele & Leydesdorff, Loet, 2016. "A triple helix model of medical innovation: Supply, demand, and technological capabilities in terms of Medical Subject Headings," Research Policy, Elsevier, vol. 45(3), pages 666-681.
    2. Kyle A McQuisten & Andrew S Peek, 2009. "Comparing Artificial Neural Networks, General Linear Models and Support Vector Machines in Building Predictive Models for Small Interfering RNAs," PLOS ONE, Public Library of Science, vol. 4(10), pages 1-14, October.
    3. Brian Hauge & Christopher Oggero & Nicole Nguyen & Changlin Fu & Fenggao Dong, 2009. "Single Tube, High Throughput Cloning of Inverted Repeat Constructs for Double-Stranded RNA Expression," PLOS ONE, Public Library of Science, vol. 4(9), pages 1-7, September.
    4. Aflaq Hamid & Sahar Saleem, 2022. "Role of nanoparticles in management of plant pathogens and scope in plant transgenics for imparting disease resistance," Plant Protection Science, Czech Academy of Agricultural Sciences, vol. 58(3), pages 173-184.
    5. Loet Leydesdorff & Lutz Bornmann, 2012. "Mapping (USPTO) patent data using overlays to Google Maps," Journal of the American Society for Information Science and Technology, Association for Information Science & Technology, vol. 63(7), pages 1442-1458, July.
    6. Chai, Sen & Menon, Anoop, 2019. "Breakthrough recognition: Bias against novelty and competition for attention," Research Policy, Elsevier, vol. 48(3), pages 733-747.
    7. Mark G Sterken & L Basten Snoek & Kobus J Bosman & Jikke Daamen & Joost A G Riksen & Jaap Bakker & Gorben P Pijlman & Jan E Kammenga, 2014. "A Heritable Antiviral RNAi Response Limits Orsay Virus Infection in Caenorhabditis elegans N2," PLOS ONE, Public Library of Science, vol. 9(2), pages 1-8, February.
    8. Loet Leydesdorff & Daniele Rotolo & Ismael Rafols, 2012. "Bibliometric perspectives on medical innovation using the medical subject Headings of PubMed," Journal of the American Society for Information Science and Technology, Association for Information Science & Technology, vol. 63(11), pages 2239-2253, November.
    9. Li-Xin Ma & Rong-Tao He & Shu-Yan Yan & Wen-Jia Yang, 2022. "RNAi Suppression of Hormone Receptor HR3 Blocks Larval Molting and Metamorphosis in the Cigarette Beetle, Lasioderma serricorne," Agriculture, MDPI, vol. 12(8), pages 1-10, August.
    10. Gersbach, Hans & Sorger, Gerhard & Amon, Christian, 2018. "Hierarchical growth: Basic and applied research," Journal of Economic Dynamics and Control, Elsevier, vol. 90(C), pages 434-459.
    11. Sen Chai, 2017. "Near Misses in the Breakthrough Discovery Process," Organization Science, INFORMS, vol. 28(3), pages 411-428, June.
    12. Shew, Aaron M. & Danforth, Diana M. & Nalley, Lawton L. & Nayga, Rodolfo M. Jr. & Tsiboe, Francis & Dixon, Bruce L., 2016. "Consumers’ Willingness-To-Pay for RNAi versus Bt Rice: Are all biotechnologies the same?," 2016 Annual Meeting, July 31-August 2, Boston, Massachusetts 235110, Agricultural and Applied Economics Association.
    13. Arnaud Segers & Joachim Carpentier & Frédéric Francis & Rudy Caparros Megido, 2023. "Gene Silencing of laccase 1 Induced by Double-Stranded RNA in Callosobruchus maculatus (Fabricius 1775) (Coleoptera: Chrysomelidae) Suggests RNAi as a Potential New Biotechnological Tool for Bruchid’s," Agriculture, MDPI, vol. 13(2), pages 1-19, February.
    14. Takasaki, Shigeru & Kawamura, Yoshihoro, 2007. "Using radial basis function networks and significance testing to select effective siRNA sequences," Computational Statistics & Data Analysis, Elsevier, vol. 51(12), pages 6476-6487, August.
    15. Ke, Qing, 2020. "The citation disadvantage of clinical research," Journal of Informetrics, Elsevier, vol. 14(1).
    16. Jiahui Zhang & Huiyuan Li & Xue Zhong & Jinfu Tian & Arnaud Segers & Lanqin Xia & Frédéric Francis, 2022. "RNA-Interference-Mediated Aphid Control in Crop Plants: A Review," Agriculture, MDPI, vol. 12(12), pages 1-20, December.
    17. Zahra Narimani & Hamid Beigy & Ashar Ahmad & Ali Masoudi-Nejad & Holger Fröhlich, 2017. "Expectation propagation for large scale Bayesian inference of non-linear molecular networks from perturbation data," PLOS ONE, Public Library of Science, vol. 12(2), pages 1-16, February.
    18. Wei-Jie Pan & Chi-Wei Chen & Yen-Wei Chu, 2011. "siPRED: Predicting siRNA Efficacy Using Various Characteristic Methods," PLOS ONE, Public Library of Science, vol. 6(11), pages 1-7, November.
    19. Ian F. Price & Jillian A. Wagner & Benjamin Pastore & Hannah L. Hertz & Wen Tang, 2023. "C. elegans germ granules sculpt both germline and somatic RNAome," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    20. Tresch Achim & Markowetz Florian, 2008. "Structure Learning in Nested Effects Models," Statistical Applications in Genetics and Molecular Biology, De Gruyter, vol. 7(1), pages 1-28, March.
    21. Joseph Nsengimana & Lander Bauters & Annelies Haegeman & Godelieve Gheysen, 2013. "Silencing of Mg - pat-10 and Mg - unc-87 in the Plant Parasitic Nematode Meloidogyne graminicola Using siRNAs," Agriculture, MDPI, vol. 3(3), pages 1-12, September.
    22. Miaowei Mao & Yajie Qian & Wenyao Zhang & Siyu Zhou & Zefeng Wang & Xianjun Chen & Yi Yang, 2023. "Controlling protein stability with SULI, a highly sensitive tag for stabilization upon light induction," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    23. Loet Leydesdorff & Floortje Alkemade & Gaston Heimeriks & Rinke Hoekstra, 2015. "Patents as instruments for exploring innovation dynamics: geographic and technological perspectives on “photovoltaic cells”," Scientometrics, Springer;Akadémiai Kiadó, vol. 102(1), pages 629-651, January.
    24. Daniele Rotolo & Ismael Rafols & Michael Hopkins & Loet Leydesdorff, 2014. "Scientometric Mapping as a Strategic Intelligence Tool for the Governance of Emerging Technologies," SPRU Working Paper Series 2014-10, SPRU - Science Policy Research Unit, University of Sussex Business School.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:nature:v:391:y:1998:i:6669:d:10.1038_35888. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.