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Production of fatty acid-derived oleochemicals and biofuels by synthetic yeast cell factories

Author

Listed:
  • Yongjin J. Zhou

    (Chalmers University of Technology
    Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology)

  • Nicolaas A. Buijs

    (Chalmers University of Technology
    Present address: Evolva Biotech, Lersø Parkallé 40-42, Copenhagen DK-2100, Denmark.)

  • Zhiwei Zhu

    (Chalmers University of Technology
    Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology)

  • Jiufu Qin

    (Chalmers University of Technology)

  • Verena Siewers

    (Chalmers University of Technology
    Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology)

  • Jens Nielsen

    (Chalmers University of Technology
    Novo Nordisk Foundation Center for Biosustainability, Chalmers University of Technology
    Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark
    Science for Life Laboratory, Royal Institute of Technology)

Abstract

Sustainable production of oleochemicals requires establishment of cell factory platform strains. The yeast Saccharomyces cerevisiae is an attractive cell factory as new strains can be rapidly implemented into existing infrastructures such as bioethanol production plants. Here we show high-level production of free fatty acids (FFAs) in a yeast cell factory, and the production of alkanes and fatty alcohols from its descendants. The engineered strain produces up to 10.4 g l−1 of FFAs, which is the highest reported titre to date. Furthermore, through screening of specific pathway enzymes, endogenous alcohol dehydrogenases and aldehyde reductases, we reconstruct efficient pathways for conversion of fatty acids to alkanes (0.8 mg l−1) and fatty alcohols (1.5 g l−1), to our knowledge the highest titres reported in S. cerevisiae. This should facilitate the construction of yeast cell factories for production of fatty acids derived products and even aldehyde-derived chemicals of high value.

Suggested Citation

  • Yongjin J. Zhou & Nicolaas A. Buijs & Zhiwei Zhu & Jiufu Qin & Verena Siewers & Jens Nielsen, 2016. "Production of fatty acid-derived oleochemicals and biofuels by synthetic yeast cell factories," Nature Communications, Nature, vol. 7(1), pages 1-9, September.
    • Handle: RePEc:nat:natcom:v:7:y:2016:i:1:d:10.1038_ncomms11709
    DOI: 10.1038/ncomms11709
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    Cited by:

    1. Wang, Kai & Da, Yangyang & Bi, Haoran & Liu, Yanhui & Chen, Biqiang & Wang, Meng & Liu, Zihe & Nielsen, Jens & Tan, Tianwei, 2023. "A one-carbon chemicals conversion strategy to produce precursor of biofuels with Saccharomyces cerevisiae," Renewable Energy, Elsevier, vol. 208(C), pages 331-340.
    2. Liu, Zihe & Moradi, Hamideh & Shi, Shuobo & Darvishi, Farshad, 2021. "Yeasts as microbial cell factories for sustainable production of biofuels," Renewable and Sustainable Energy Reviews, Elsevier, vol. 143(C).
    3. Shan Yang & Ruibing Chen & Xuan Cao & Guodong Wang & Yongjin J. Zhou, 2024. "De novo biosynthesis of the hops bioactive flavonoid xanthohumol in yeast," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    4. Das, Manali & Patra, Pradipta & Ghosh, Amit, 2020. "Metabolic engineering for enhancing microbial biosynthesis of advanced biofuels," Renewable and Sustainable Energy Reviews, Elsevier, vol. 119(C).

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