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The adaptor protein AP-3β disassembles heat-induced stress granules via 19S regulatory particle in Arabidopsis

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  • Lei Pang

    (Southern University of Science and Technology)

  • Yuanzhi Huang

    (Southern University of Science and Technology)

  • Yilin He

    (Southern University of Science and Technology)

  • Dong Jiang

    (Southern University of Science and Technology)

  • Ruixi Li

    (Southern University of Science and Technology)

Abstract

To survive under adverse conditions, plants form stress granules (SGs) to temporally store mRNA and halt translation as a primary response. Dysregulation in SG disassembly can have detrimental effects on plant survival after stress release, yet the underlying mechanism remains poorly understood. Using Arabidopsis as a model system, we demonstrate that the β subunit of adaptor protein (AP) -3 complex (AP-3β) interacts with the SG core RNA-binding proteins Tudor staphylococcal nuclease 1/2 (TSN1/2) both in vitro and in vivo. We also show that AP-3β is rapidly recruited to SGs upon heat induction and plays a key role in disassembling SGs during stress recovery. Genetic evidences support that AP-3β serves as an adaptor to recruit the 19S regulatory particle (RP) of the proteasome to SGs. Notably, the 19S RP promotes SG disassembly through RP-associated deubiquitylation, independent of its proteolytic activity. This deubiquitylation process of SG components is crucial for translation reinitiation and growth recovery after heat release. Our findings uncover a previously unexplored role of the 19S RP in regulating SG disassembly and highlights the importance of endomembrane proteins in supporting RNA granule dynamics in plants.

Suggested Citation

  • Lei Pang & Yuanzhi Huang & Yilin He & Dong Jiang & Ruixi Li, 2025. "The adaptor protein AP-3β disassembles heat-induced stress granules via 19S regulatory particle in Arabidopsis," Nature Communications, Nature, vol. 16(1), pages 1-20, December.
  • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-025-57306-7
    DOI: 10.1038/s41467-025-57306-7
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    References listed on IDEAS

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