IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v16y2025i1d10.1038_s41467-024-55647-3.html
   My bibliography  Save this article

NHSL3 controls single and collective cell migration through two distinct mechanisms

Author

Listed:
  • Nikita M. Novikov

    (Institut Polytechnique de Paris)

  • Jinmei Gao

    (Institute for Integrative Biology of the Cell (I2BC))

  • Artem I. Fokin

    (Institut Polytechnique de Paris)

  • Nathalie Rocques

    (Institut Polytechnique de Paris)

  • Giovanni Chiappetta

    (LPC CNRS UMR8249)

  • Karina D. Rysenkova

    (Institut Polytechnique de Paris)

  • Diego Javier Zea

    (Institute for Integrative Biology of the Cell (I2BC))

  • Anna Polesskaya

    (Institut Polytechnique de Paris)

  • Joelle Vinh

    (LPC CNRS UMR8249)

  • Raphael Guerois

    (Institute for Integrative Biology of the Cell (I2BC))

  • Alexis M. Gautreau

    (Institut Polytechnique de Paris)

Abstract

The molecular mechanisms underlying cell migration remain incompletely understood. Here, we show that knock-out cells for NHSL3, the most recently identified member of the Nance-Horan Syndrome family, are more persistent than parental cells in single cell migration, but that, in wound healing, follower cells are impaired in their ability to follow leader cells. The NHSL3 locus encodes several isoforms. We identify the partner repertoire of each isoform using proteomics and predict direct partners and their binding sites using an AlphaFold2-based pipeline. Rescue with specific isoforms, and lack of rescue when relevant binding sites are mutated, establish that the interaction of a long isoform with MENA/VASP proteins is critical at cell-cell junctions for collective migration, while the interaction of a short one with 14-3-3θ in lamellipodia is critical for single cell migration. Taken together, these results demonstrate that NHSL3 regulates single and collective cell migration through distinct mechanisms.

Suggested Citation

  • Nikita M. Novikov & Jinmei Gao & Artem I. Fokin & Nathalie Rocques & Giovanni Chiappetta & Karina D. Rysenkova & Diego Javier Zea & Anna Polesskaya & Joelle Vinh & Raphael Guerois & Alexis M. Gautreau, 2025. "NHSL3 controls single and collective cell migration through two distinct mechanisms," Nature Communications, Nature, vol. 16(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-024-55647-3
    DOI: 10.1038/s41467-024-55647-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-55647-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-55647-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:16:y:2025:i:1:d:10.1038_s41467-024-55647-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.