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A unique symbiosome in an anaerobic single-celled eukaryote

Author

Listed:
  • Jon Jerlström-Hultqvist

    (Uppsala Universitet
    Dalhousie University)

  • Lucie Gallot-Lavallée

    (Dalhousie University)

  • Dayana E. Salas-Leiva

    (Dalhousie University
    University of Cambridge)

  • Bruce A. Curtis

    (Dalhousie University)

  • Kristína Záhonová

    (University of Alberta
    Czech Academy of Sciences
    BIOCEV
    University of Ostrava)

  • Ivan Čepička

    (Charles University)

  • Courtney W. Stairs

    (Lund University)

  • Shweta Pipaliya

    (University of Alberta)

  • Joel B. Dacks

    (University of Alberta
    Czech Academy of Sciences
    University College)

  • John M. Archibald

    (Dalhousie University)

  • Andrew J. Roger

    (Dalhousie University)

Abstract

Symbiotic relationships between eukaryotes and prokaryotes played pivotal roles in the evolution of life and drove the emergence of specialized symbiotic structures in animals, plants and fungi. The host-evolved symbiotic structures of microbial eukaryotes – the vast majority of such hosts in nature – remain largely unstudied. Here we describe highly structured symbiosomes within three free-living anaerobic protists (Anaeramoeba spp.). We dissect this symbiosis using complete genome sequencing and transcriptomics of host and symbiont cells coupled with fluorescence in situ hybridization, and 3D reconstruction using focused-ion-beam scanning electron microscopy. The emergence of the symbiosome is underpinned by expansion of gene families encoding regulators of membrane trafficking and phagosomal maturation and extensive bacteria-to-eukaryote lateral transfer. The symbionts reside deep within a symbiosomal membrane network that enables metabolic syntrophy by precisely positioning sulfate-reducing bacteria alongside host hydrogenosomes. Importantly, the symbionts maintain connections to the Anaeramoeba plasma membrane, blurring traditional boundaries between ecto- and endosymbiosis.

Suggested Citation

  • Jon Jerlström-Hultqvist & Lucie Gallot-Lavallée & Dayana E. Salas-Leiva & Bruce A. Curtis & Kristína Záhonová & Ivan Čepička & Courtney W. Stairs & Shweta Pipaliya & Joel B. Dacks & John M. Archibald , 2024. "A unique symbiosome in an anaerobic single-celled eukaryote," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-54102-7
    DOI: 10.1038/s41467-024-54102-7
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    References listed on IDEAS

    as
    1. Dayana E. Salas-Leiva & Eelco C. Tromer & Bruce A. Curtis & Jon Jerlström-Hultqvist & Martin Kolisko & Zhenzhen Yi & Joan S. Salas-Leiva & Lucie Gallot-Lavallée & Shelby K. Williams & Geert J. P. L. K, 2021. "Genomic analysis finds no evidence of canonical eukaryotic DNA processing complexes in a free-living protist," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
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    4. Dayana E. Salas-Leiva & Eelco C. Tromer & Bruce A. Curtis & Jon Jerlström-Hultqvist & Martin Kolisko & Zhenzhen Yi & Joan S. Salas-Leiva & Lucie Gallot-Lavallée & Shelby K. Williams & Geert J. P. L. K, 2021. "Author Correction: Genomic analysis finds no evidence of canonical eukaryotic DNA processing complexes in a free-living protist," Nature Communications, Nature, vol. 12(1), pages 1-6, December.
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