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Mutations of PDS5 genes enhance TAD-like domain formation in Arabidopsis thaliana

Author

Listed:
  • Anna-Maria Göbel

    (University of Hohenheim)

  • Sida Zhou

    (University of Potsdam
    Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
    Max Planck Institute of Molecular Plant Physiology)

  • Zhidan Wang

    (University of Hohenheim)

  • Sofia Tzourtzou

    (University of Hohenheim)

  • Axel Himmelbach

    (Leibniz Institute of Plant Genetics and Crop Plant Research (IPK))

  • Shiwei Zheng

    (University of Potsdam
    Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
    Max Planck Institute of Molecular Plant Physiology)

  • Mónica Pradillo

    (Universidad Complutense)

  • Chang Liu

    (University of Hohenheim)

  • Hua Jiang

    (University of Potsdam
    Leibniz Institute of Plant Genetics and Crop Plant Research (IPK)
    Max Planck Institute of Molecular Plant Physiology)

Abstract

In eukaryotes, topologically associating domains (TADs) organize the genome into functional compartments. While TAD-like structures are common in mammals and many plants, they are challenging to detect in Arabidopsis thaliana. Here, we demonstrate that Arabidopsis PDS5 proteins play a negative role in TAD-like domain formation. Through Hi-C analysis, we show that mutations in PDS5 genes lead to the widespread emergence of enhanced TAD-like domains throughout the Arabidopsis genome, excluding pericentromeric regions. These domains exhibit increased chromatin insulation and enhanced chromatin interactions, without significant changes in gene expression or histone modifications. Our results suggest that PDS5 proteins are key regulators of genome architecture, influencing 3D chromatin organization independently of transcriptional activity. This study provides insights into the unique chromatin structure of Arabidopsis and the broader mechanisms governing plant genome folding.

Suggested Citation

  • Anna-Maria Göbel & Sida Zhou & Zhidan Wang & Sofia Tzourtzou & Axel Himmelbach & Shiwei Zheng & Mónica Pradillo & Chang Liu & Hua Jiang, 2024. "Mutations of PDS5 genes enhance TAD-like domain formation in Arabidopsis thaliana," Nature Communications, Nature, vol. 15(1), pages 1-8, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53760-x
    DOI: 10.1038/s41467-024-53760-x
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