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Affinity molecular assay for detecting Candida albicans using chitin affinity and RPA-CRISPR/Cas12a

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  • Shimei Shen

    (Chongqing Medical University
    The First Affiliated Hospital of Chongqing Medical University
    Chongqing Hospital of Traditional Chinese Medicine
    Chongqing Medical University)

  • Wen Wang

    (Chongqing Medical University
    The First Affiliated Hospital of Chongqing Medical University
    The Second Affiliated Hospital of Chengdu Medical College (Nuclear Industry 416 Hospital))

  • Yuanyan Ma

    (Chongqing Medical University
    Chongqing Medical University)

  • Shilei Wang

    (Chongqing Hospital of Traditional Chinese Medicine)

  • Shaocheng Zhang

    (The Second Affiliated Hospital of Chengdu Medical College (Nuclear Industry 416 Hospital)
    Chengdu Medical College)

  • Xuefei Cai

    (Chongqing Medical University)

  • Liang Chen

    (University at Buffalo)

  • Jin Zhang

    (Chongqing Medical University
    Chongqing Medical University)

  • Yalan Li

    (Chongqing Medical University
    Chongqing Medical University)

  • Xiaoli Wu

    (Chongqing Medical University
    Chongqing Medical University)

  • Jie Wei

    (Zhuhai People’s Hospital (Zhuhai Hospital Affiliated with Jinan University))

  • Yanan Zhao

    (University at Buffalo)

  • Ailong Huang

    (Chongqing Medical University)

  • Siqiang Niu

    (The First Affiliated Hospital of Chongqing Medical University)

  • Deqiang Wang

    (Chongqing Medical University
    Chongqing Medical University
    Chongqing National Biomedicine Industry Park)

Abstract

Invasive fungal infections (IFIs) pose a significant threat to immunocompromised individuals, leading to considerable morbidity and mortality. Prompt and accurate diagnosis is essential for effective treatment. Here we develop a rapid molecular diagnostic method that involves three steps: fungal enrichment using affinity-magnetic separation (AMS), genomic DNA extraction with silicon hydroxyl magnetic beads, and detection through a one-pot system. This method, optimized to detect 30 CFU/mL of C. albicans in blood and bronchoalveolar lavage (BAL) samples within 2.5 h, is approximately 100 times more sensitive than microscopy-based staining. Initial validation using clinical samples showed 93.93% sensitivity, 100% specificity, and high predictive values, while simulated tests demonstrated 95% sensitivity and 100% specificity. This cost-effective, highly sensitive technique offers potential for use in resource-limited clinical settings and can be easily adapted to differentiate between fungal species and detect drug resistance.

Suggested Citation

  • Shimei Shen & Wen Wang & Yuanyan Ma & Shilei Wang & Shaocheng Zhang & Xuefei Cai & Liang Chen & Jin Zhang & Yalan Li & Xiaoli Wu & Jie Wei & Yanan Zhao & Ailong Huang & Siqiang Niu & Deqiang Wang, 2024. "Affinity molecular assay for detecting Candida albicans using chitin affinity and RPA-CRISPR/Cas12a," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53693-5
    DOI: 10.1038/s41467-024-53693-5
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    References listed on IDEAS

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    1. Xiong Ding & Kun Yin & Ziyue Li & Rajesh V. Lalla & Enrique Ballesteros & Maroun M. Sfeir & Changchun Liu, 2020. "Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
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