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Empowering the on-site detection of nucleic acids by integrating CRISPR and digital signal processing

Author

Listed:
  • Chang Yeol Lee

    (Massachusetts General Hospital Research Institute
    Massachusetts General Hospital, Harvard Medical School
    Korea Research Institute of Bioscience and Biotechnology (KRIBB))

  • Hyunho Kim

    (Massachusetts General Hospital Research Institute
    Massachusetts General Hospital, Harvard Medical School)

  • Ismail Degani

    (Massachusetts General Hospital Research Institute
    Massachusetts Institute of Technology)

  • Hanna Lee

    (Massachusetts General Hospital Research Institute)

  • Angel Sandoval

    (Massachusetts General Hospital Research Institute)

  • Yoonho Nam

    (Massachusetts General Hospital Research Institute
    Korea Advanced Institute of Science and Technology (KAIST))

  • Madeleine Pascavis

    (Massachusetts General Hospital Research Institute
    Northeastern University)

  • Hyun Gyu Park

    (Korea Advanced Institute of Science and Technology (KAIST))

  • Thomas Randall

    (Massachusetts General Hospital)

  • Amy Ly

    (Massachusetts General Hospital, Harvard Medical School)

  • Cesar M. Castro

    (Massachusetts General Hospital Research Institute
    Massachusetts General Hospital, Harvard Medical School)

  • Hakho Lee

    (Massachusetts General Hospital Research Institute
    Massachusetts General Hospital, Harvard Medical School)

Abstract

Addressing the global disparity in cancer care necessitates the development of rapid and affordable nucleic acid (NA) testing technologies. This need is particularly critical for cervical cancer, where molecular detection of human papillomavirus (HPV) has emerged as an accurate screening method. However, implementing this transition in low- and middle-income countries has been challenging due to the high costs and centralized facilities required for current NA tests. Here, we present CreDiT (CRISPR Enhanced Digital Testing) for on-site NA detection. The CreDiT platform integrates i) a one-pot CRISPR strategy that simultaneously amplifies both target NAs and analytical signals and ii) a robust fluorescent detection based on digital communication (encoding/decoding) technology. These features enable a rapid assay (

Suggested Citation

  • Chang Yeol Lee & Hyunho Kim & Ismail Degani & Hanna Lee & Angel Sandoval & Yoonho Nam & Madeleine Pascavis & Hyun Gyu Park & Thomas Randall & Amy Ly & Cesar M. Castro & Hakho Lee, 2024. "Empowering the on-site detection of nucleic acids by integrating CRISPR and digital signal processing," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50588-3
    DOI: 10.1038/s41467-024-50588-3
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    References listed on IDEAS

    as
    1. Zhichen Xu & Dongjuan Chen & Tao Li & Jiayu Yan & Jiang Zhu & Ting He & Rui Hu & Ying Li & Yunhuang Yang & Maili Liu, 2022. "Microfluidic space coding for multiplexed nucleic acid detection via CRISPR-Cas12a and recombinase polymerase amplification," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    2. Xiong Ding & Kun Yin & Ziyue Li & Rajesh V. Lalla & Enrique Ballesteros & Maroun M. Sfeir & Changchun Liu, 2020. "Ultrasensitive and visual detection of SARS-CoV-2 using all-in-one dual CRISPR-Cas12a assay," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    Full references (including those not matched with items on IDEAS)

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