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Dynamically cultured, differentiated bovine adipose-derived stem cell spheroids as building blocks for biofabricating cultured fat

Author

Listed:
  • Annemarie Klatt

    (Reutlingen University)

  • Jannis O. Wollschlaeger

    (Reutlingen University)

  • Franziska B. Albrecht

    (Reutlingen University)

  • Sara Rühle

    (Reutlingen University)

  • Lena B. Holzwarth

    (Reutlingen University)

  • Holger Hrenn

    (University of Hohenheim)

  • Tanja Melzer

    (University of Hohenheim)

  • Simon Heine

    (Reutlingen University)

  • Petra J. Kluger

    (Reutlingen University)

Abstract

Cultured or cultivated meat, animal muscle, and fat tissue grown in vitro, could transform the global meat market, reducing animal suffering while using fewer resources than traditional meat production and no antimicrobials at all. To ensure the appeal of cultured meat to future customers, cultured fat is essential for achieving desired mouthfeel, taste, and texture, especially in beef. In this work we show the establishment of primary bovine adipose-derived stem cell spheroids in static and dynamic suspension culture. Spheroids are successfully differentiated using a single-step protocol. Differentiated spheroids from dynamic cultures maintain stability and viability during 3D bioprinting in edible gellan gum. Also, the fatty acid composition of differentiated spheroids is significantly different from control spheroids. The cells are cultured antibiotic-free to minimize the use of harmful substances. This work presents a stable and bioprintable building block for cultured fat with a high cell density in a 3D dynamic cell culture system.

Suggested Citation

  • Annemarie Klatt & Jannis O. Wollschlaeger & Franziska B. Albrecht & Sara Rühle & Lena B. Holzwarth & Holger Hrenn & Tanja Melzer & Simon Heine & Petra J. Kluger, 2024. "Dynamically cultured, differentiated bovine adipose-derived stem cell spheroids as building blocks for biofabricating cultured fat," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53486-w
    DOI: 10.1038/s41467-024-53486-w
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    References listed on IDEAS

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    1. Peter Carmeliet & Rakesh K. Jain, 2000. "Angiogenesis in cancer and other diseases," Nature, Nature, vol. 407(6801), pages 249-257, September.
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