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A single-dose circular RNA vaccine prevents Zika virus infection without enhancing dengue severity in mice

Author

Listed:
  • Xinglong Liu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Zhengfeng Li

    (Chinese Academy of Sciences)

  • Xiaoxia Li

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Weixuan Wu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Huadong Jiang

    (Chinese Academy of Sciences
    University of Science and Technology of China)

  • Yufen Zheng

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Junjie Zhou

    (Chinese Academy of Sciences)

  • Xianmiao Ye

    (Sun Yat-sen University)

  • Junnan Lu

    (Chinese Academy of Sciences)

  • Wei Wang

    (Bioland Laboratory)

  • Lei Yu

    (Guangzhou Medical University)

  • Yiping Li

    (Sun Yat-sen University)

  • Linbing Qu

    (Chinese Academy of Sciences)

  • Jianhua Wang

    (Chinese Academy of Sciences)

  • Feng Li

    (Guangzhou Medical University)

  • Ling Chen

    (Chinese Academy of Sciences
    Guangzhou National Laboratory)

  • Linping Wu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Liqiang Feng

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

Abstract

Antibody-dependent enhancement (ADE) is a potential concern for the development of Zika virus (ZIKV) vaccines. Cross-reactive but poorly neutralizing antibodies, usually targeting viral pre-membrane or envelope (E) proteins, can potentially enhance dengue virus (DENV) infection. Although E domain III (EDIII) contains ZIKV-specific epitopes, its immunogenicity is poor. Here, we show that dimeric EDIII, fused to human IgG1 Fc fragment (EDIII-Fc) and encoded by circular RNA (circRNA), induces better germinal center reactions and higher neutralizing antibodies compared to circRNAs encoding monomeric or trimeric EDIII. Two doses of circRNAs encoding EDIII-Fc and ZIKV nonstructural protein NS1, another protective antigen, prevent lethal ZIKV infection in neonates born to immunized C57BL/6 mice and in interferon-α/β receptor knockout adult C57BL/6 mice. Importantly, a single-dose optimized circRNA vaccine with improved antigen expression confers potent and durable protection without inducing obvious DENV ADE in mice, laying the groundwork for developing flavivirus vaccines based on circRNAs encoding EDIII-Fc and NS1.

Suggested Citation

  • Xinglong Liu & Zhengfeng Li & Xiaoxia Li & Weixuan Wu & Huadong Jiang & Yufen Zheng & Junjie Zhou & Xianmiao Ye & Junnan Lu & Wei Wang & Lei Yu & Yiping Li & Linbing Qu & Jianhua Wang & Feng Li & Ling, 2024. "A single-dose circular RNA vaccine prevents Zika virus infection without enhancing dengue severity in mice," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-53242-0
    DOI: 10.1038/s41467-024-53242-0
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    References listed on IDEAS

    as
    1. Theodore C. Pierson & Michael S. Diamond, 2018. "The emergence of Zika virus and its new clinical syndromes," Nature, Nature, vol. 560(7720), pages 573-581, August.
    2. Gopal Sapparapu & Estefania Fernandez & Nurgun Kose & Bin Cao & Julie M. Fox & Robin G. Bombardi & Haiyan Zhao & Christopher A. Nelson & Aubrey L. Bryan & Trevor Barnes & Edgar Davidson & Indira U. My, 2016. "Neutralizing human antibodies prevent Zika virus replication and fetal disease in mice," Nature, Nature, vol. 540(7633), pages 443-447, December.
    3. Elie Dolgin, 2023. "Why rings of RNA could be the next blockbuster drug," Nature, Nature, vol. 622(7981), pages 22-24, October.
    4. R. Alexander Wesselhoeft & Piotr S. Kowalski & Daniel G. Anderson, 2018. "Engineering circular RNA for potent and stable translation in eukaryotic cells," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    5. Alex W. Wessel & Nurgun Kose & Robin G. Bombardi & Vicky Roy & Warangkana Chantima & Juthathip Mongkolsapaya & Melissa A. Edeling & Christopher A. Nelson & Irene Bosch & Galit Alter & Gavin R. Screato, 2020. "Antibodies targeting epitopes on the cell-surface form of NS1 protect against Zika virus infection during pregnancy," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
    6. Mark J. Bailey & James Duehr & Harrison Dulin & Felix Broecker & Julia A. Brown & Fortuna O. Arumemi & Maria C. Bermúdez González & Victor H. Leyva-Grado & Matthew J. Evans & Viviana Simon & Jean K. L, 2018. "Human antibodies targeting Zika virus NS1 provide protection against disease in a mouse model," Nature Communications, Nature, vol. 9(1), pages 1-11, December.
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