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Galectins induced from hemocytes bridge phosphatidylserine and N-glycosylated Drpr/CED-1 receptor during dendrite pruning

Author

Listed:
  • Hsin-Ho Sung

    (Academia Sinica)

  • Hsun Li

    (Academia Sinica)

  • Yi-Chun Huang

    (Academia Sinica)

  • Chun-Lu Ai

    (Academia Sinica)

  • Ming-Yen Hsieh

    (Academia Sinica)

  • Hau-Ming Jan

    (Academia Sinica)

  • Yu-Ju Peng

    (Academia Sinica)

  • Hsien-Ya Lin

    (Academia Sinica)

  • Chih-Hsuan Yeh

    (Academia Sinica)

  • Shu-Yu Lin

    (Academia Sinica)

  • Chun-Yen Yeh

    (Academia Sinica)

  • Ying-Ju Cheng

    (Academia Sinica)

  • Kay-Hooi Khoo

    (Academia Sinica)

  • Chun-Hung Lin

    (Academia Sinica)

  • Cheng-Ting Chien

    (Academia Sinica
    Academia Sinica)

Abstract

During neuronal pruning, phagocytes engulf shed cellular debris to avoid inflammation and maintain tissue homeostasis. How phagocytic receptors recognize degenerating neurites had been unclear. Here, we identify two glucosyltransferases Alg8 and Alg10 of the N-glycosylation pathway required for dendrite fragmentation and clearance through genetic screen. The scavenger receptor Draper (Drpr) is N-glycosylated with complex- or hybrid-type N-glycans that interact specifically with galectins. We also identify the galectins Crouching tiger (Ctg) and Hidden dragon (Hdg) that interact with N-glycosylated Drpr and function in dendrite pruning via the Drpr pathway. Ctg and Hdg are required in hemocytes for expression and function, and are induced during dendrite injury to localize to injured dendrites through specific interaction with exposed phosphatidylserine (PS) on the surface membrane of injured dendrites. Thus, the galectins Ctg and Hdg bridge the interaction between PS and N-glycosylated Drpr, leading to the activation of phagocytosis.

Suggested Citation

  • Hsin-Ho Sung & Hsun Li & Yi-Chun Huang & Chun-Lu Ai & Ming-Yen Hsieh & Hau-Ming Jan & Yu-Ju Peng & Hsien-Ya Lin & Chih-Hsuan Yeh & Shu-Yu Lin & Chun-Yen Yeh & Ying-Ju Cheng & Kay-Hooi Khoo & Chun-Hung, 2024. "Galectins induced from hemocytes bridge phosphatidylserine and N-glycosylated Drpr/CED-1 receptor during dendrite pruning," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51581-6
    DOI: 10.1038/s41467-024-51581-6
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    1. Won-Suk Chung & Laura E. Clarke & Gordon X. Wang & Benjamin K. Stafford & Alexander Sher & Chandrani Chakraborty & Julia Joung & Lynette C. Foo & Andrew Thompson & Chinfei Chen & Stephen J. Smith & Be, 2013. "Astrocytes mediate synapse elimination through MEGF10 and MERTK pathways," Nature, Nature, vol. 504(7480), pages 394-400, December.
    2. Kalpana Makhijani & Brandy Alexander & Deepti Rao & Sophia Petraki & Leire Herboso & Katelyn Kukar & Itrat Batool & Stephanie Wachner & Katrina S. Gold & Corinna Wong & Michael B. O’Connor & Katja Brü, 2017. "Regulation of Drosophila hematopoietic sites by Activin-β from active sensory neurons," Nature Communications, Nature, vol. 8(1), pages 1-12, December.
    3. Rikinari Hanayama & Masato Tanaka & Keiko Miwa & Azusa Shinohara & Akihiro Iwamatsu & Shigekazu Nagata, 2002. "Identification of a factor that links apoptotic cells to phagocytes," Nature, Nature, vol. 417(6885), pages 182-187, May.
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