IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-51581-6.html
   My bibliography  Save this article

Galectins induced from hemocytes bridge phosphatidylserine and N-glycosylated Drpr/CED-1 receptor during dendrite pruning

Author

Listed:
  • Hsin-Ho Sung

    (Academia Sinica)

  • Hsun Li

    (Academia Sinica)

  • Yi-Chun Huang

    (Academia Sinica)

  • Chun-Lu Ai

    (Academia Sinica)

  • Ming-Yen Hsieh

    (Academia Sinica)

  • Hau-Ming Jan

    (Academia Sinica)

  • Yu-Ju Peng

    (Academia Sinica)

  • Hsien-Ya Lin

    (Academia Sinica)

  • Chih-Hsuan Yeh

    (Academia Sinica)

  • Shu-Yu Lin

    (Academia Sinica)

  • Chun-Yen Yeh

    (Academia Sinica)

  • Ying-Ju Cheng

    (Academia Sinica)

  • Kay-Hooi Khoo

    (Academia Sinica)

  • Chun-Hung Lin

    (Academia Sinica)

  • Cheng-Ting Chien

    (Academia Sinica
    Academia Sinica)

Abstract

During neuronal pruning, phagocytes engulf shed cellular debris to avoid inflammation and maintain tissue homeostasis. How phagocytic receptors recognize degenerating neurites had been unclear. Here, we identify two glucosyltransferases Alg8 and Alg10 of the N-glycosylation pathway required for dendrite fragmentation and clearance through genetic screen. The scavenger receptor Draper (Drpr) is N-glycosylated with complex- or hybrid-type N-glycans that interact specifically with galectins. We also identify the galectins Crouching tiger (Ctg) and Hidden dragon (Hdg) that interact with N-glycosylated Drpr and function in dendrite pruning via the Drpr pathway. Ctg and Hdg are required in hemocytes for expression and function, and are induced during dendrite injury to localize to injured dendrites through specific interaction with exposed phosphatidylserine (PS) on the surface membrane of injured dendrites. Thus, the galectins Ctg and Hdg bridge the interaction between PS and N-glycosylated Drpr, leading to the activation of phagocytosis.

Suggested Citation

  • Hsin-Ho Sung & Hsun Li & Yi-Chun Huang & Chun-Lu Ai & Ming-Yen Hsieh & Hau-Ming Jan & Yu-Ju Peng & Hsien-Ya Lin & Chih-Hsuan Yeh & Shu-Yu Lin & Chun-Yen Yeh & Ying-Ju Cheng & Kay-Hooi Khoo & Chun-Hung, 2024. "Galectins induced from hemocytes bridge phosphatidylserine and N-glycosylated Drpr/CED-1 receptor during dendrite pruning," Nature Communications, Nature, vol. 15(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51581-6
    DOI: 10.1038/s41467-024-51581-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-51581-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-51581-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Won-Suk Chung & Laura E. Clarke & Gordon X. Wang & Benjamin K. Stafford & Alexander Sher & Chandrani Chakraborty & Julia Joung & Lynette C. Foo & Andrew Thompson & Chinfei Chen & Stephen J. Smith & Be, 2013. "Astrocytes mediate synapse elimination through MEGF10 and MERTK pathways," Nature, Nature, vol. 504(7480), pages 394-400, December.
    2. Kalpana Makhijani & Brandy Alexander & Deepti Rao & Sophia Petraki & Leire Herboso & Katelyn Kukar & Itrat Batool & Stephanie Wachner & Katrina S. Gold & Corinna Wong & Michael B. O’Connor & Katja Brü, 2017. "Regulation of Drosophila hematopoietic sites by Activin-β from active sensory neurons," Nature Communications, Nature, vol. 8(1), pages 1-12, December.
    3. Rikinari Hanayama & Masato Tanaka & Keiko Miwa & Azusa Shinohara & Akihiro Iwamatsu & Shigekazu Nagata, 2002. "Identification of a factor that links apoptotic cells to phagocytes," Nature, Nature, vol. 417(6885), pages 182-187, May.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Samuel S. Kim & Buu Truong & Karthik Jagadeesh & Kushal K. Dey & Amber Z. Shen & Soumya Raychaudhuri & Manolis Kellis & Alkes L. Price, 2024. "Leveraging single-cell ATAC-seq and RNA-seq to identify disease-critical fetal and adult brain cell types," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    2. Christian F. Christensen & Quentin Laurichesse & Rihab Loudhaief & Julien Colombani & Ditte S. Andersen, 2024. "Drosophila activins adapt gut size to food intake and promote regenerative growth," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    3. Danyang Chen & Qianqian Lou & Xiang-Jie Song & Fang Kang & An Liu & Changjian Zheng & Yanhua Li & Di Wang & Sen Qun & Zhi Zhang & Peng Cao & Yan Jin, 2024. "Microglia govern the extinction of acute stress-induced anxiety-like behaviors in male mice," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    4. Tony Marion & Husni Elbahesh & Paul G Thomas & John P DeVincenzo & Richard Webby & Klaus Schughart, 2016. "Respiratory Mucosal Proteome Quantification in Human Influenza Infections," PLOS ONE, Public Library of Science, vol. 11(4), pages 1-16, April.
    5. Hidenori Tabata & Megumi Sasaki & Masakazu Agetsuma & Hitomi Sano & Yuki Hirota & Michio Miyajima & Kanehiro Hayashi & Takao Honda & Masashi Nishikawa & Yutaka Inaguma & Hidenori Ito & Hirohide Takeba, 2022. "Erratic and blood vessel-guided migration of astrocyte progenitors in the cerebral cortex," Nature Communications, Nature, vol. 13(1), pages 1-21, December.
    6. Johnna Doherty & Amy E Sheehan & Rachel Bradshaw & A Nicole Fox & Tsai-Yi Lu & Marc R Freeman, 2014. "PI3K Signaling and Stat92E Converge to Modulate Glial Responsiveness to Axonal Injury," PLOS Biology, Public Library of Science, vol. 12(11), pages 1-16, November.
    7. Xiaojing Shi & Longlong Luo & Jixian Wang & Hui Shen & Yongfang Li & Muyassar Mamtilahun & Chang Liu & Rubing Shi & Joon-Hyuk Lee & Hengli Tian & Zhijun Zhang & Yongting Wang & Won-Suk Chung & Yaohui , 2021. "Stroke subtype-dependent synapse elimination by reactive gliosis in mice," Nature Communications, Nature, vol. 12(1), pages 1-19, December.
    8. Ikuko Takeda & Kohei Yoshihara & Dennis L. Cheung & Tomoko Kobayashi & Masakazu Agetsuma & Makoto Tsuda & Kei Eto & Schuichi Koizumi & Hiroaki Wake & Andrew J. Moorhouse & Junichi Nabekura, 2022. "Controlled activation of cortical astrocytes modulates neuropathic pain-like behaviour," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    9. Panpan Zhang & Masahiro Maruoka & Ryo Suzuki & Hikaru Katani & Yu Dou & Daniel M. Packwood & Hidetaka Kosako & Motomu Tanaka & Jun Suzuki, 2023. "Extracellular calcium functions as a molecular glue for transmembrane helices to activate the scramblase Xkr4," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    10. Ting Wan & Wusheng Zhu & Ying Zhao & Xiaohao Zhang & Ruidong Ye & Meng Zuo & Pengfei Xu & Zhenqian Huang & Chunni Zhang & Yi Xie & Xinfeng Liu, 2022. "Astrocytic phagocytosis contributes to demyelination after focal cortical ischemia in mice," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51581-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.