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Circular RNAs regulate neuron size and migration of midbrain dopamine neurons during development

Author

Listed:
  • Mateja Rybiczka-Tešulov

    (University Medical Center Utrecht, Utrecht University
    Medical University of Vienna)

  • Oxana Garritsen

    (University Medical Center Utrecht, Utrecht University)

  • Morten T. Venø

    (Interdisciplinary Nanoscience Centre, Aarhus University
    Omiics ApS)

  • Laura Wieg

    (University Medical Center Utrecht, Utrecht University)

  • Roland van Dijk

    (University Medical Center Utrecht, Utrecht University
    VectorY Therapeutics, Matrix Innovation Center VI)

  • Karim Rahimi

    (Interdisciplinary Nanoscience Centre, Aarhus University
    Harvard Medical School)

  • Andreia Gomes-Duarte

    (University Medical Center Utrecht, Utrecht University
    VectorY Therapeutics, Matrix Innovation Center VI)

  • Marina de Wit

    (University Medical Center Utrecht, Utrecht University)

  • Lieke L. Haar

    (University Medical Center Utrecht, Utrecht University
    Max Delbrück Center)

  • Lars Michels

    (University Medical Center Utrecht, Utrecht University
    VectorY Therapeutics, Matrix Innovation Center VI)

  • Nicky C. H. van Kronenburg

    (University Medical Center Utrecht, Utrecht University)

  • Christiaan Meer

    (University Medical Center Utrecht, Utrecht University)

  • Jørgen Kjems

    (Interdisciplinary Nanoscience Centre, Aarhus University)

  • Vamshidhar R. Vangoor

    (University Medical Center Utrecht, Utrecht University)

  • R. Jeroen Pasterkamp

    (University Medical Center Utrecht, Utrecht University)

Abstract

Midbrain dopamine (mDA) neurons play an essential role in cognitive and motor behaviours and are linked to different brain disorders. However, the molecular mechanisms underlying their development, and in particular the role of non-coding RNAs (ncRNAs), remain incompletely understood. Here, we establish the transcriptomic landscape and alternative splicing patterns of circular RNAs (circRNAs) at key developmental timepoints in mouse mDA neurons in vivo using fluorescence-activated cell sorting followed by short- and long-read RNA sequencing. In situ hybridisation shows expression of several circRNAs during early mDA neuron development and post-transcriptional silencing unveils roles for different circRNAs in regulating mDA neuron morphology. Finally, in utero electroporation and time-lapse imaging implicate circRmst, a circRNA with widespread morphological effects, in the migration of developing mDA neurons in vivo. Together, these data for the first time suggest a functional role for circRNAs in developing mDA neurons and characterise poorly defined aspects of mDA neuron development.

Suggested Citation

  • Mateja Rybiczka-Tešulov & Oxana Garritsen & Morten T. Venø & Laura Wieg & Roland van Dijk & Karim Rahimi & Andreia Gomes-Duarte & Marina de Wit & Lieke L. Haar & Lars Michels & Nicky C. H. van Kronenb, 2024. "Circular RNAs regulate neuron size and migration of midbrain dopamine neurons during development," Nature Communications, Nature, vol. 15(1), pages 1-23, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-51041-1
    DOI: 10.1038/s41467-024-51041-1
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    References listed on IDEAS

    as
    1. Sebastian Memczak & Marvin Jens & Antigoni Elefsinioti & Francesca Torti & Janna Krueger & Agnieszka Rybak & Luisa Maier & Sebastian D. Mackowiak & Lea H. Gregersen & Mathias Munschauer & Alexander Lo, 2013. "Circular RNAs are a large class of animal RNAs with regulatory potency," Nature, Nature, vol. 495(7441), pages 333-338, March.
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    3. Thomas B. Hansen & Trine I. Jensen & Bettina H. Clausen & Jesper B. Bramsen & Bente Finsen & Christian K. Damgaard & Jørgen Kjems, 2013. "Natural RNA circles function as efficient microRNA sponges," Nature, Nature, vol. 495(7441), pages 384-388, March.
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