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Germline β−1,3-glucan deposits are required for female gametogenesis in Arabidopsis thaliana

Author

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  • Sara C. Pinto

    (Universidade do Porto, rua do Campo Alegre s/n
    University of Adelaide, Waite Campus
    John Innes Centre, Norwich Research Park)

  • Weng Herng Leong

    (University of Adelaide, Waite Campus
    University of Adelaide)

  • Hweiting Tan

    (University of Adelaide, Waite Campus
    University of Adelaide)

  • Lauren McKee

    (KTH Royal Institute of Technology)

  • Amelie Prevost

    (University of Adelaide)

  • Chao Ma

    (University of Adelaide, Waite Campus)

  • Neil. J. Shirley

    (University of Adelaide, Waite Campus
    University of Adelaide)

  • Rosanna Petrella

    (Università Degli Studi di Milano)

  • Xiujuan Yang

    (University of Adelaide, Waite Campus)

  • Anna M. Koltunow

    (The University of Queensland)

  • Vincent Bulone

    (University of Adelaide, Waite Campus
    University of Adelaide
    KTH Royal Institute of Technology
    Flinders University)

  • Masahiro M. Kanaoka

    (Nagoya University, Furo-cho, Chikusa-ku
    Prefectural University of Hiroshima)

  • Tetsuya Higashyiama

    (Nagoya University, Furo-cho, Chikusa-ku
    The University of Tokyo)

  • Sílvia Coimbra

    (Universidade do Porto, rua do Campo Alegre s/n)

  • Matthew R. Tucker

    (University of Adelaide, Waite Campus)

Abstract

Correct regulation of intercellular communication is a fundamental requirement for cell differentiation. In Arabidopsis thaliana, the female germline differentiates from a single somatic ovule cell that becomes encased in β−1,3-glucan, a water insoluble polysaccharide implicated in limiting pathogen invasion, regulating intercellular trafficking in roots, and promoting pollen development. Whether β−1,3-glucan facilitates germline isolation and development has remained contentious, since limited evidence is available to support a functional role. Here, transcriptional profiling of adjoining germline and somatic cells revealed differences in gene expression related to β−1,3-glucan metabolism and signalling through intercellular channels (plasmodesmata). Dominant expression of a β−1,3-glucanase in the female germline transiently perturbed β−1,3-glucan deposits, allowed intercellular movement of tracer molecules, and led to changes in germline gene expression and histone marks, eventually leading to termination of germline development. Our findings indicate that germline β−1,3-glucan fulfils a functional role in the ovule by insulating the primary germline cell, and thereby determines the success of downstream female gametogenesis.

Suggested Citation

  • Sara C. Pinto & Weng Herng Leong & Hweiting Tan & Lauren McKee & Amelie Prevost & Chao Ma & Neil. J. Shirley & Rosanna Petrella & Xiujuan Yang & Anna M. Koltunow & Vincent Bulone & Masahiro M. Kanaoka, 2024. "Germline β−1,3-glucan deposits are required for female gametogenesis in Arabidopsis thaliana," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-50143-0
    DOI: 10.1038/s41467-024-50143-0
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    References listed on IDEAS

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    2. Fran Supek & Matko Bošnjak & Nives Škunca & Tomislav Šmuc, 2011. "REVIGO Summarizes and Visualizes Long Lists of Gene Ontology Terms," PLOS ONE, Public Library of Science, vol. 6(7), pages 1-9, July.
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