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Reduced alcohol preference and intake after fecal transplant in patients with alcohol use disorder is transmissible to germ-free mice

Author

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  • Jennifer T. Wolstenholme

    (Virginia Commonwealth University)

  • Justin M. Saunders

    (Virginia Commonwealth University)

  • Maren Smith

    (Virginia Commonwealth University)

  • Jason D. Kang

    (Virginia Commonwealth University)

  • Phillip B. Hylemon

    (Virginia Commonwealth University)

  • Javier González-Maeso

    (Virginia Commonwealth University)

  • Andrew Fagan

    (Virginia Commonwealth University and Richmond VA Medical Center)

  • Derrick Zhao

    (Virginia Commonwealth University)

  • Masoumeh Sikaroodi

    (George Mason University)

  • Jeremy Herzog

    (University of North Carolina at Chapel Hill)

  • Amirhossein Shamsaddini

    (George Mason University)

  • Marcela Peña-Rodríguez

    (University of Guadalajara)

  • Lianyong Su

    (Virginia Commonwealth University)

  • Yun-Ling Tai

    (Virginia Commonwealth University)

  • Jing Zheng

    (Virginia Commonwealth University)

  • Po-Cheng Cheng

    (Virginia Commonwealth University)

  • R. Balfour Sartor

    (University of North Carolina at Chapel Hill
    University of North Carolina at Chapel Hill)

  • Patrick M. Gillevet

    (George Mason University)

  • Huiping Zhou

    (Virginia Commonwealth University)

  • Jasmohan S. Bajaj

    (Virginia Commonwealth University and Richmond VA Medical Center)

Abstract

Alcohol use disorder is a major cause of morbidity, which requires newer treatment approaches. We previously showed in a randomized clinical trial that alcohol craving and consumption reduces after fecal transplantation. Here, to determine if this could be transmitted through microbial transfer, germ-free male C57BL/6 mice received stool or sterile supernatants collected from the trial participants pre-/post-fecal transplant. We found that mice colonized with post-fecal transplant stool but not supernatants reduced ethanol acceptance, intake and preference versus pre-fecal transplant colonized mice. Microbial taxa that were higher in post-fecal transplant humans were also associated with lower murine alcohol intake and preference. A majority of the differentially expressed genes (immune response, inflammation, oxidative stress response, and epithelial cell proliferation) occurred in the intestine rather than the liver and prefrontal cortex. These findings suggest a potential for therapeutically targeting gut microbiota and the microbial-intestinal interface to alter gut-liver-brain axis and reduce alcohol consumption in humans.

Suggested Citation

  • Jennifer T. Wolstenholme & Justin M. Saunders & Maren Smith & Jason D. Kang & Phillip B. Hylemon & Javier González-Maeso & Andrew Fagan & Derrick Zhao & Masoumeh Sikaroodi & Jeremy Herzog & Amirhossei, 2022. "Reduced alcohol preference and intake after fecal transplant in patients with alcohol use disorder is transmissible to germ-free mice," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34054-6
    DOI: 10.1038/s41467-022-34054-6
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    References listed on IDEAS

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