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iPS cell generation-associated point mutations include many C > T substitutions via different cytosine modification mechanisms

Author

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  • Ryoko Araki

    (National Institutes for Quantum Science and Technology
    National Institutes for Quantum Science and Technology)

  • Tomo Suga

    (National Institutes for Quantum Science and Technology
    National Institutes for Quantum Science and Technology)

  • Yuko Hoki

    (National Institutes for Quantum Science and Technology
    National Institutes for Quantum Science and Technology)

  • Kaori Imadome

    (National Institutes for Quantum Science and Technology
    National Institutes for Quantum Science and Technology)

  • Misato Sunayama

    (National Institutes for Quantum Science and Technology
    National Institutes for Quantum Science and Technology)

  • Satoshi Kamimura

    (National Institutes for Quantum Science and Technology
    National Institutes for Quantum Science and Technology)

  • Mayumi Fujita

    (National Institutes for Quantum Science and Technology
    National Institutes for Quantum Science and Technology)

  • Masumi Abe

    (National Institutes for Quantum Science and Technology)

Abstract

Genomic aberrations are a critical impediment for the safe medical use of iPSCs and their origin and developmental mechanisms remain unknown. Here we find through WGS analysis of human and mouse iPSC lines that genomic mutations are de novo events and that, in addition to unmodified cytosine base prone to deamination, the DNA methylation sequence CpG represents a significant mutation-prone site. CGI and TSS regions show increased mutations in iPSCs and elevated mutations are observed in retrotransposons, especially in the AluY subfamily. Furthermore, increased cytosine to thymine mutations are observed in differentially methylated regions. These results indicate that in addition to deamination of cytosine, demethylation of methylated cytosine, which plays a central role in genome reprogramming, may act mutagenically during iPSC generation.

Suggested Citation

  • Ryoko Araki & Tomo Suga & Yuko Hoki & Kaori Imadome & Misato Sunayama & Satoshi Kamimura & Mayumi Fujita & Masumi Abe, 2024. "iPS cell generation-associated point mutations include many C > T substitutions via different cytosine modification mechanisms," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-49335-5
    DOI: 10.1038/s41467-024-49335-5
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