IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-48831-y.html
   My bibliography  Save this article

9-fold symmetry is not essential for centriole elongation and formation of new centriole-like structures

Author

Listed:
  • Pallavi Panda

    (California Institute of Technology)

  • Mark S. Ladinsky

    (California Institute of Technology)

  • David M. Glover

    (California Institute of Technology)

Abstract

As daughter centrioles assemble during G2, they recruit conserved Ana3/RTTN followed by its partner Rcd4/PPP1R35. Together, this contributes to the subsequent recruitment of Ana1/CEP295, required for the centriole’s conversion to a centrosome. Here, we show that Rcd4/PPP1R35 is also required to maintain 9-fold centriole symmetry in the Drosophila male germline; its absence causes microtubule triplets to disperse into a reduced number of doublet or singlet microtubules. rcd4-null mutant spermatocytes display skinny centrioles that elongate normally and localize centriolar components correctly. Mutant spermatocytes also have centrioles of normal girth that splay at their proximal ends when induced to elongate by Ana1 overexpression. Skinny and splayed spermatid centrioles can still recruit a proximal centriole-like (PCL) structure marking a capability to initiate features of centriole duplication in developing sperm. Thus, stable 9-fold symmetry of microtubule triplets is not essential for centriole growth, correct longitudinal association of centriole components, and aspects of centriole duplication.

Suggested Citation

  • Pallavi Panda & Mark S. Ladinsky & David M. Glover, 2024. "9-fold symmetry is not essential for centriole elongation and formation of new centriole-like structures," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48831-y
    DOI: 10.1038/s41467-024-48831-y
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-48831-y
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-48831-y?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Nikola S. Dzhindzhev & Quan D. Yu & Kipp Weiskopf & George Tzolovsky & Ines Cunha-Ferreira & Maria Riparbelli & Ana Rodrigues-Martins & Monica Bettencourt-Dias & Giuliano Callaini & David M. Glover, 2010. "Asterless is a scaffold for the onset of centriole assembly," Nature, Nature, vol. 467(7316), pages 714-718, October.
    2. Jayachandran Gopalakrishnan & Vito Mennella & Stephanie Blachon & Bo Zhai & Andrew H. Smith & Timothy L. Megraw & Daniela Nicastro & Steven P. Gygi & David A. Agard & Tomer Avidor-Reiss, 2011. "Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome," Nature Communications, Nature, vol. 2(1), pages 1-11, September.
    3. Laurence Pelletier & Eileen O’Toole & Anne Schwager & Anthony A. Hyman & Thomas Müller-Reichert, 2006. "Centriole assembly in Caenorhabditis elegans," Nature, Nature, vol. 444(7119), pages 619-623, November.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.

      More about this item

      Statistics

      Access and download statistics

      Corrections

      All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48831-y. See general information about how to correct material in RePEc.

      If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

      If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

      If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

      For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

      Please note that corrections may take a couple of weeks to filter through the various RePEc services.

      IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.