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Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome

Author

Listed:
  • Jayachandran Gopalakrishnan

    (Harvard Medical School)

  • Vito Mennella

    (University of California)

  • Stephanie Blachon

    (Harvard Medical School)

  • Bo Zhai

    (Harvard Medical School)

  • Andrew H. Smith

    (Harvard Medical School)

  • Timothy L. Megraw

    (College of Medicine, Florida State University)

  • Daniela Nicastro

    (Brandeis University)

  • Steven P. Gygi

    (Harvard Medical School)

  • David A. Agard

    (University of California)

  • Tomer Avidor-Reiss

    (Harvard Medical School)

Abstract

Centrosomes are conserved organelles that are essential for accurate cell division and cilium formation. A centrosome consists of a pair of centrioles surrounded by a protein network of pericentriolar material (PCM) that is essential for the centrosome's function. In this study, we show that Sas-4 provides a scaffold for cytoplasmic complexes (named S-CAP), which include CNN, Asl and D-PLP, proteins that are all found in the centrosomes at the vicinity of the centriole. When Sas-4 is absent, nascent procentrioles are unstable and lack PCM, and functional centrosomes are not generated. When Sas-4 is mutated, so that it cannot form S-CAP complexes, centrosomes are present but with dramatically reduced levels of PCM. Finally, purified S-CAP complexes or recombinant Sas-4 can bind centrosomes stripped of PCM, whereas recombinant CNN or Asl cannot. In summary, PCM assembly begins in the cytosol where Sas-4 provides a scaffold for pre-assembled cytoplasmic complexes before tethering of the complexes in a centrosome.

Suggested Citation

  • Jayachandran Gopalakrishnan & Vito Mennella & Stephanie Blachon & Bo Zhai & Andrew H. Smith & Timothy L. Megraw & Daniela Nicastro & Steven P. Gygi & David A. Agard & Tomer Avidor-Reiss, 2011. "Sas-4 provides a scaffold for cytoplasmic complexes and tethers them in a centrosome," Nature Communications, Nature, vol. 2(1), pages 1-11, September.
    • Handle: RePEc:nat:natcom:v:2:y:2011:i:1:d:10.1038_ncomms1367
    DOI: 10.1038/ncomms1367
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    Cited by:

    1. Pallavi Panda & Mark S. Ladinsky & David M. Glover, 2024. "9-fold symmetry is not essential for centriole elongation and formation of new centriole-like structures," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

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