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Nuclear actin structure regulates chromatin accessibility

Author

Listed:
  • Buer Sen

    (University of North Carolina)

  • Zhihui Xie

    (University of North Carolina)

  • Michelle D. Thomas

    (University of North Carolina at Chapel Hill)

  • Samantha G. Pattenden

    (University of North Carolina at Chapel Hill)

  • Sean Howard

    (Boise State University)

  • Cody McGrath

    (University of North Carolina)

  • Maya Styner

    (University of North Carolina)

  • Gunes Uzer

    (Boise State University)

  • Terrence S. Furey

    (University of North Carolina at Chapel Hill)

  • Janet Rubin

    (University of North Carolina)

Abstract

Polymerized β-actin may provide a structural basis for chromatin accessibility and actin transport into the nucleus can guide mesenchymal stem cell (MSC) differentiation. Using MSC, we show that using CK666 to inhibit Arp2/3 directed secondary actin branching results in decreased nuclear actin structure, and significantly alters chromatin access measured with ATACseq at 24 h. The ATAC-seq results due to CK666 are distinct from those caused by cytochalasin D (CytoD), which enhances nuclear actin structure. In addition, nuclear visualization shows Arp2/3 inhibition decreases pericentric H3K9me3 marks. CytoD, alternatively, induces redistribution of H3K27me3 marks centrally. Such alterations in chromatin landscape are consistent with differential gene expression associated with distinctive differentiation patterns. Further, knockdown of the non-enzymatic monomeric actin binding protein, Arp4, leads to extensive chromatin unpacking, but only a modest increase in transcription, indicating an active role for actin-Arp4 in transcription. These data indicate that dynamic actin remodeling can regulate chromatin interactions.

Suggested Citation

  • Buer Sen & Zhihui Xie & Michelle D. Thomas & Samantha G. Pattenden & Sean Howard & Cody McGrath & Maya Styner & Gunes Uzer & Terrence S. Furey & Janet Rubin, 2024. "Nuclear actin structure regulates chromatin accessibility," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48580-y
    DOI: 10.1038/s41467-024-48580-y
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    References listed on IDEAS

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    3. Ying Wang & Alice Sherrard & Bing Zhao & Michael Melak & Jonathan Trautwein & Eva-Maria Kleinschnitz & Nikolaos Tsopoulidis & Oliver T. Fackler & Carsten Schwan & Robert Grosse, 2019. "GPCR-induced calcium transients trigger nuclear actin assembly for chromatin dynamics," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
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