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Two noncompeting human neutralizing antibodies targeting MPXV B6 show protective effects against orthopoxvirus infections

Author

Listed:
  • Runchu Zhao

    (Institute of Microbiology, Chinese Academy of Sciences)

  • Lili Wu

    (Institute of Microbiology, Chinese Academy of Sciences)

  • Junqing Sun

    (Institute of Microbiology, Chinese Academy of Sciences
    Shanxi Agricultural University)

  • Dezhi Liu

    (Institute of Microbiology, Chinese Academy of Sciences
    Anhui University)

  • Pu Han

    (Institute of Microbiology, Chinese Academy of Sciences)

  • Yue Gao

    (Institute of Microbiology, Chinese Academy of Sciences
    Hebei University, Baoding)

  • Yi Zhang

    (Institute of Microbiology, Chinese Academy of Sciences
    Anhui University)

  • Yanli Xu

    (Capital Medical University)

  • Xiao Qu

    (Institute of Microbiology, Chinese Academy of Sciences)

  • Han Wang

    (Peking University)

  • Yan Chai

    (Institute of Microbiology, Chinese Academy of Sciences)

  • Zhihai Chen

    (Capital Medical University)

  • George F. Gao

    (Institute of Microbiology, Chinese Academy of Sciences
    Shanxi Agricultural University
    University of Chinese Academy of Sciences)

  • Qihui Wang

    (Institute of Microbiology, Chinese Academy of Sciences
    Anhui University
    University of Chinese Academy of Sciences)

Abstract

The recent outbreak of mpox epidemic, caused by monkeypox virus (MPXV), poses a new threat to global public health. Here, we initially assessed the preexisting antibody level to the MPXV B6 protein in vaccinia vaccinees born before the end of the immunization program and then identified two monoclonal antibodies (MAbs), hMB621 and hMB668, targeting distinct epitopes on B6, from one vaccinee. Binding assays demonstrate that both MAbs exhibit broad binding abilities to B6 and its orthologs in vaccinia (VACV), variola (VARV) and cowpox viruses (CPXV). Neutralizing assays reveal that the two MAbs showed potent neutralization against VACV. Animal experiments using a BALB/c female mouse model indicate that the two MAbs showed effective protection against VACV via intraperitoneal injection. Additionally, we determined the complex structure of B6 and hMB668, revealing the structural feature of B6 and the epitope of hMB668. Collectively, our study provides two promising antibody candidates for the treatment of orthopoxvirus infections, including mpox.

Suggested Citation

  • Runchu Zhao & Lili Wu & Junqing Sun & Dezhi Liu & Pu Han & Yue Gao & Yi Zhang & Yanli Xu & Xiao Qu & Han Wang & Yan Chai & Zhihai Chen & George F. Gao & Qihui Wang, 2024. "Two noncompeting human neutralizing antibodies targeting MPXV B6 show protective effects against orthopoxvirus infections," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-48312-2
    DOI: 10.1038/s41467-024-48312-2
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    References listed on IDEAS

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    3. John Jumper & Richard Evans & Alexander Pritzel & Tim Green & Michael Figurnov & Olaf Ronneberger & Kathryn Tunyasuvunakool & Russ Bates & Augustin Žídek & Anna Potapenko & Alex Bridgland & Clemens Me, 2021. "Highly accurate protein structure prediction with AlphaFold," Nature, Nature, vol. 596(7873), pages 583-589, August.
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