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Cortex-wide transcranial localization microscopy with fluorescently labeled red blood cells

Author

Listed:
  • Quanyu Zhou

    (University of Zurich
    ETH Zurich)

  • Chaim Glück

    (University of Zurich
    Zurich Neuroscience Center)

  • Lin Tang

    (University of Zurich
    ETH Zurich)

  • Lukas Glandorf

    (University of Zurich
    ETH Zurich)

  • Jeanne Droux

    (Zurich Neuroscience Center
    University Hospital and University of Zurich)

  • Mohamad El Amki

    (Zurich Neuroscience Center
    University Hospital and University of Zurich)

  • Susanne Wegener

    (Zurich Neuroscience Center
    University Hospital and University of Zurich)

  • Bruno Weber

    (University of Zurich
    Zurich Neuroscience Center)

  • Daniel Razansky

    (University of Zurich
    ETH Zurich
    Zurich Neuroscience Center)

  • Zhenyue Chen

    (University of Zurich
    ETH Zurich)

Abstract

Large-scale imaging of brain activity with high spatio-temporal resolution is crucial for advancing our understanding of brain function. The existing neuroimaging techniques are largely limited by restricted field of view, slow imaging speed, or otherwise do not have the adequate spatial resolution to capture brain activities on a capillary and cellular level. To address these limitations, we introduce fluorescence localization microscopy aided with sparsely-labeled red blood cells for cortex-wide morphological and functional cerebral angiography with 4.9 µm spatial resolution and 1 s temporal resolution. When combined with fluorescence calcium imaging, the proposed method enables extended recordings of stimulus-evoked neuro-vascular changes in the murine brain while providing simultaneous multiparametric readings of intracellular neuronal activity, blood flow velocity/direction/volume, and vessel diameter. Owing to its simplicity and versatility, the proposed approach will become an invaluable tool for deciphering the regulation of cortical microcirculation and neurovascular coupling in health and disease.

Suggested Citation

  • Quanyu Zhou & Chaim Glück & Lin Tang & Lukas Glandorf & Jeanne Droux & Mohamad El Amki & Susanne Wegener & Bruno Weber & Daniel Razansky & Zhenyue Chen, 2024. "Cortex-wide transcranial localization microscopy with fluorescently labeled red blood cells," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-47892-3
    DOI: 10.1038/s41467-024-47892-3
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    References listed on IDEAS

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    1. Davide Boido & Ravi L. Rungta & Bruno-Félix Osmanski & Morgane Roche & Tomokazu Tsurugizawa & Denis Bihan & Luisa Ciobanu & Serge Charpak, 2019. "Mesoscopic and microscopic imaging of sensory responses in the same animal," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    2. Jianian Lin & Zongyue Cheng & Guang Yang & Meng Cui, 2022. "Optical gearbox enabled versatile multiscale high-throughput multiphoton functional imaging," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
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