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Mesoscopic and microscopic imaging of sensory responses in the same animal

Author

Listed:
  • Davide Boido

    (Université Paris Descartes)

  • Ravi L. Rungta

    (Université Paris Descartes)

  • Bruno-Félix Osmanski

    (Université Paris Descartes)

  • Morgane Roche

    (Université Paris Descartes)

  • Tomokazu Tsurugizawa

    (NeuroSpin, Bât 145, Commissariat à l’Energie Atomique-Saclay Center)

  • Denis Bihan

    (NeuroSpin, Bât 145, Commissariat à l’Energie Atomique-Saclay Center)

  • Luisa Ciobanu

    (NeuroSpin, Bât 145, Commissariat à l’Energie Atomique-Saclay Center)

  • Serge Charpak

    (Université Paris Descartes)

Abstract

Imaging based on blood flow dynamics is widely used to study sensory processing. Here we investigated the extent to which local neuronal and capillary responses (two-photon microscopy) are correlated to mesoscopic responses detected with fast ultrasound (fUS) and BOLD-fMRI. Using a specialized chronic olfactory bulb preparation, we report that sequential imaging of the same mouse allows quantitative comparison of odour responses, imaged at both microscopic and mesoscopic scales. Under these conditions, functional hyperaemia occurred at the threshold of neuronal activation and fUS-CBV signals could be detected at the level of single voxels with activation maps varying according to blood velocity. Both neuronal and vascular responses increase non-linearly as a function of odour concentration, whereas both microscopic and mesoscopic vascular responses are linearly correlated to local neuronal calcium. These data establish strengths and limits of mesoscopic imaging techniques to report neural activity.

Suggested Citation

  • Davide Boido & Ravi L. Rungta & Bruno-Félix Osmanski & Morgane Roche & Tomokazu Tsurugizawa & Denis Bihan & Luisa Ciobanu & Serge Charpak, 2019. "Mesoscopic and microscopic imaging of sensory responses in the same animal," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-019-09082-4
    DOI: 10.1038/s41467-019-09082-4
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    Cited by:

    1. Grant R. Gordon, 2024. "Neurovascular coupling during hypercapnia in cerebral blood flow regulation," Nature Communications, Nature, vol. 15(1), pages 1-3, December.
    2. Quanyu Zhou & Chaim Glück & Lin Tang & Lukas Glandorf & Jeanne Droux & Mohamad El Amki & Susanne Wegener & Bruno Weber & Daniel Razansky & Zhenyue Chen, 2024. "Cortex-wide transcranial localization microscopy with fluorescently labeled red blood cells," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    3. Marine Tournissac & Emmanuelle Chaigneau & Sonia Pfister & Ali-Kemal Aydin & Yannick Goulam Houssen & Philip O’Herron & Jessica Filosa & Mayeul Collot & Anne Joutel & Serge Charpak, 2024. "Neurovascular coupling and CO2 interrogate distinct vascular regulations," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    4. Quanyu Zhou & Zhenyue Chen & Yu-Hang Liu & Mohamad El Amki & Chaim Glück & Jeanne Droux & Michael Reiss & Bruno Weber & Susanne Wegener & Daniel Razansky, 2022. "Three-dimensional wide-field fluorescence microscopy for transcranial mapping of cortical microcirculation," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

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