IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v15y2024i1d10.1038_s41467-024-46825-4.html
   My bibliography  Save this article

Ion mobility-tandem mass spectrometry of mucin-type O-glycans

Author

Listed:
  • Leïla Bechtella

    (Freie Universität Berlin
    Fritz Haber Institute of the Max Planck Society)

  • Jin Chunsheng

    (University of Gothenburg)

  • Kerstin Fentker

    (Freie Universität Berlin
    Max Delbrück Center for Molecular Medicine)

  • Güney R. Ertürk

    (Freie Universität Berlin)

  • Marc Safferthal

    (Freie Universität Berlin
    Fritz Haber Institute of the Max Planck Society)

  • Łukasz Polewski

    (Freie Universität Berlin
    Fritz Haber Institute of the Max Planck Society)

  • Michael Götze

    (Freie Universität Berlin
    Fritz Haber Institute of the Max Planck Society)

  • Simon Y. Graeber

    (corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin
    German Center for Lung Research (DZL)
    Berlin Institute of Health at Charité – Universitätsmedizin Berlin)

  • Gaël M. Vos

    (Freie Universität Berlin
    Fritz Haber Institute of the Max Planck Society)

  • Weston B. Struwe

    (University of Oxford
    University of Oxford)

  • Marcus A. Mall

    (corporate member of Freie Universität Berlin and Humboldt-Universität zu Berlin
    German Center for Lung Research (DZL)
    Berlin Institute of Health at Charité – Universitätsmedizin Berlin)

  • Philipp Mertins

    (Max Delbrück Center for Molecular Medicine
    Berlin Institute of Health)

  • Niclas G. Karlsson

    (University of Gothenburg
    Oslo Metropolitan University)

  • Kevin Pagel

    (Freie Universität Berlin
    Fritz Haber Institute of the Max Planck Society)

Abstract

The dense O-glycosylation of mucins plays an important role in the defensive properties of the mucus hydrogel. Aberrant glycosylation is often correlated with inflammation and pathology such as COPD, cancer, and Crohn’s disease. The inherent complexity of glycans and the diversity in the O-core structure constitute fundamental challenges for the analysis of mucin-type O-glycans. Due to coexistence of multiple isomers, multidimensional workflows such as LC-MS are required. To separate the highly polar carbohydrates, porous graphitized carbon is often used as a stationary phase. However, LC-MS workflows are time-consuming and lack reproducibility. Here we present a rapid alternative for separating and identifying O-glycans released from mucins based on trapped ion mobility mass spectrometry. Compared to established LC-MS, the acquisition time is reduced from an hour to two minutes. To test the validity, the developed workflow was applied to sputum samples from cystic fibrosis patients to map O-glycosylation features associated with disease.

Suggested Citation

  • Leïla Bechtella & Jin Chunsheng & Kerstin Fentker & Güney R. Ertürk & Marc Safferthal & Łukasz Polewski & Michael Götze & Simon Y. Graeber & Gaël M. Vos & Weston B. Struwe & Marcus A. Mall & Philipp M, 2024. "Ion mobility-tandem mass spectrometry of mucin-type O-glycans," Nature Communications, Nature, vol. 15(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46825-4
    DOI: 10.1038/s41467-024-46825-4
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-024-46825-4
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-024-46825-4?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Gaёl M. Vos & Kevin C. Hooijschuur & Zeshi Li & John Fjeldsted & Christian Klein & Robert P. Vries & Javier Sastre Toraño & Geert-Jan Boons, 2023. "Sialic acid O-acetylation patterns and glycosidic linkage type determination by ion mobility-mass spectrometry," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    2. J. Hofmann & H. S. Hahm & P. H. Seeberger & K. Pagel, 2015. "Identification of carbohydrate anomers using ion mobility–mass spectrometry," Nature, Nature, vol. 526(7572), pages 241-244, October.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Xiaoyu Zhou & Zhuofan Wang & Jingjin Fan & Zheng Ouyang, 2023. "High-resolution separation of bioisomers using ion cloud profiling," Nature Communications, Nature, vol. 14(1), pages 1-6, December.
    2. Minmin Li & Yuting Xiong & Yuchen Cao & Chen Zhang & Yuting Li & Hanwen Ning & Fan Liu & Han Zhou & Xiaonong Li & Xianlong Ye & Yue Pang & Jiaming Zhang & Xinmiao Liang & Guangyan Qing, 2023. "Identification of tagged glycans with a protein nanopore," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    3. Shuling Xu & Zhijun Zhu & Daniel G. Delafield & Michael J. Rigby & Gaoyuan Lu & Megan Braun & Luigi Puglielli & Lingjun Li, 2024. "Spatially and temporally probing distinctive glycerophospholipid alterations in Alzheimer’s disease mouse brain via high-resolution ion mobility-enabled sn-position resolved lipidomics," Nature Communications, Nature, vol. 15(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-46825-4. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.