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Open-source microscope add-on for structured illumination microscopy

Author

Listed:
  • Mélanie T. M. Hannebelle

    (Swiss Federal Institute of Technology (EPFL)
    Swiss Federal Institute of Technology (EPFL)
    Stanford University)

  • Esther Raeth

    (Swiss Federal Institute of Technology (EPFL)
    Swiss Federal Institute of Technology (EPFL))

  • Samuel M. Leitao

    (Swiss Federal Institute of Technology (EPFL))

  • Tomáš Lukeš

    (Swiss Federal Institute of Technology (EPFL))

  • Jakub Pospíšil

    (Czech Technical University in Prague
    UiT The Arctic University of Norway)

  • Chiara Toniolo

    (Swiss Federal Institute of Technology (EPFL))

  • Olivier F. Venzin

    (Swiss Federal Institute of Technology (EPFL))

  • Antonius Chrisnandy

    (Swiss Federal Institute of Technology (EPFL))

  • Prabhu P. Swain

    (Swiss Federal Institute of Technology (EPFL))

  • Nathan Ronceray

    (Swiss Federal Institute of Technology (EPFL))

  • Matthias P. Lütolf

    (Swiss Federal Institute of Technology (EPFL))

  • Andrew C. Oates

    (Swiss Federal Institute of Technology (EPFL))

  • Guy M. Hagen

    (University of Colorado Colorado Springs)

  • Theo Lasser

    (Swiss Federal Institute of Technology (EPFL))

  • Aleksandra Radenovic

    (Swiss Federal Institute of Technology (EPFL))

  • John D. McKinney

    (Swiss Federal Institute of Technology (EPFL))

  • Georg E. Fantner

    (Swiss Federal Institute of Technology (EPFL))

Abstract

Super-resolution techniques expand the abilities of researchers who have the knowledge and resources to either build or purchase a system. This excludes the part of the research community without these capabilities. Here we introduce the openSIM add-on to upgrade existing optical microscopes to Structured Illumination super-resolution Microscopes (SIM). The openSIM is an open-hardware system, designed and documented to be easily duplicated by other laboratories, making super-resolution modality accessible to facilitate innovative research. The add-on approach gives a performance improvement for pre-existing lab equipment without the need to build a completely new system.

Suggested Citation

  • Mélanie T. M. Hannebelle & Esther Raeth & Samuel M. Leitao & Tomáš Lukeš & Jakub Pospíšil & Chiara Toniolo & Olivier F. Venzin & Antonius Chrisnandy & Prabhu P. Swain & Nathan Ronceray & Matthias P. L, 2024. "Open-source microscope add-on for structured illumination microscopy," Nature Communications, Nature, vol. 15(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45567-7
    DOI: 10.1038/s41467-024-45567-7
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    References listed on IDEAS

    as
    1. Nikolce Gjorevski & Norman Sachs & Andrea Manfrin & Sonja Giger & Maiia E. Bragina & Paloma Ordóñez-Morán & Hans Clevers & Matthias P. Lutolf, 2016. "Designer matrices for intestinal stem cell and organoid culture," Nature, Nature, vol. 539(7630), pages 560-564, November.
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