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A Gamma-adapted subunit vaccine induces broadly neutralizing antibodies against SARS-CoV-2 variants and protects mice from infection

Author

Listed:
  • Lorena M. Coria

    (Universidad Nacional de San Martín (UNSAM) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad Nacional de San Martín)

  • Juan Manuel Rodriguez

    (Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires
    Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires)

  • Agostina Demaria

    (Universidad Nacional de San Martín (UNSAM) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad Nacional de San Martín)

  • Laura A. Bruno

    (Universidad Nacional de San Martín (UNSAM) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad Nacional de San Martín)

  • Mayra Rios Medrano

    (Universidad Nacional de San Martín (UNSAM) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad Nacional de San Martín)

  • Celeste Pueblas Castro

    (Universidad Nacional de San Martín (UNSAM) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad Nacional de San Martín)

  • Eliana F. Castro

    (Universidad Nacional de San Martín (UNSAM) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad Nacional de San Martín)

  • Sabrina A. Del Priore

    (Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires)

  • Andres C. Hernando Insua

    (Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires
    Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires)

  • Ingrid G. Kaufmann

    (Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires)

  • Lucas M. Saposnik

    (Universidad Nacional de San Martín (UNSAM) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad Nacional de San Martín)

  • William B. Stone

    (College of Agriculture and Life Sciences, Fralin Life Science Institute, Virginia Polytechnic Institute and State University)

  • Lineia Prado

    (Universidad Nacional de San Martín (UNSAM) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad Nacional de San Martín)

  • Ulises S. Notaro

    (Universidad Nacional del Litoral-CONICET; Esperanza)

  • Ayelen N. Amweg

    (Universidad Nacional del Litoral-CONICET; Esperanza)

  • Pablo U. Diaz

    (Universidad Nacional del Litoral-CONICET; Esperanza)

  • Martin Avaro

    (Instituto Nacional de Enfermedades Infecciosas - ANLIS “Dr. Carlos G. Malbrán”. Ciudad Autónoma de Buenos Aires)

  • Hugo Ortega

    (Universidad Nacional del Litoral-CONICET; Esperanza)

  • Ana Ceballos

    (Instituto de Investigaciones Biomédicas en Retrovirus y SIDA, INBIRS-CONICET)

  • Valeria Krum

    (Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires)

  • Francisco M. Zurvarra

    (Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires
    Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires)

  • Johanna E. Sidabra

    (Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires)

  • Ignacio Drehe

    (Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires)

  • Jonathan A. Baqué

    (Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires)

  • Mariana Li Causi

    (Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires)

  • Analia V. De Nichilo

    (Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires
    Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires)

  • Cristian J. Payes

    (Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires)

  • Teresa Southard

    (College of Agriculture and Life Sciences, Fralin Life Science Institute, Virginia Polytechnic Institute and State University)

  • Julio C. Vega

    (Ciudad Autónoma de Buenos Aires)

  • Albert J. Auguste

    (College of Agriculture and Life Sciences, Fralin Life Science Institute, Virginia Polytechnic Institute and State University
    Virginia Polytechnic Institute and State University)

  • Diego E. Álvarez

    (Universidad Nacional de San Martín (UNSAM) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad Nacional de San Martín)

  • Juan M. Flo

    (Unidad de I+D de Biofármacos, Ciudad Autónoma de Buenos Aires)

  • Karina A. Pasquevich

    (Universidad Nacional de San Martín (UNSAM) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad Nacional de San Martín)

  • Juliana Cassataro

    (Universidad Nacional de San Martín (UNSAM) – Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET)
    Universidad Nacional de San Martín)

Abstract

In the context of continuous emergence of SARS-CoV-2 variants of concern (VOCs), one strategy to prevent the severe outcomes of COVID-19 is developing safe and effective broad-spectrum vaccines. Here, we present preclinical studies of a RBD vaccine derived from the Gamma SARS-CoV-2 variant adjuvanted with Alum. The Gamma-adapted RBD vaccine is more immunogenic than the Ancestral RBD vaccine in terms of inducing broader neutralizing antibodies. The Gamma RBD presents more immunogenic B-cell restricted epitopes and induces a higher proportion of specific-B cells and plasmablasts than the Ancestral RBD version. The Gamma-adapted vaccine induces antigen specific T cell immune responses and confers protection against Ancestral and Omicron BA.5 SARS-CoV-2 challenge in mice. Moreover, the Gamma RBD vaccine induces higher and broader neutralizing antibody activity than homologous booster vaccination in mice previously primed with different SARS-CoV-2 vaccine platforms. Our study indicates that the adjuvanted Gamma RBD vaccine is highly immunogenic and a broad-spectrum vaccine candidate.

Suggested Citation

  • Lorena M. Coria & Juan Manuel Rodriguez & Agostina Demaria & Laura A. Bruno & Mayra Rios Medrano & Celeste Pueblas Castro & Eliana F. Castro & Sabrina A. Del Priore & Andres C. Hernando Insua & Ingrid, 2024. "A Gamma-adapted subunit vaccine induces broadly neutralizing antibodies against SARS-CoV-2 variants and protects mice from infection," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
  • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-024-45180-8
    DOI: 10.1038/s41467-024-45180-8
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    References listed on IDEAS

    as
    1. Karina A. Pasquevich & Lorena M. Coria & Ana Ceballos & Bianca Mazzitelli & Juan Manuel Rodriguez & Agostina Demaría & Celeste Pueblas Castro & Laura Bruno & Lucas Saposnik & Melina Salvatori & August, 2023. "Safety and immunogenicity of a SARS-CoV-2 Gamma variant RBD-based protein adjuvanted vaccine used as booster in healthy adults," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    2. Rahul K. Suryawanshi & Irene P. Chen & Tongcui Ma & Abdullah M. Syed & Noah Brazer & Prachi Saldhi & Camille R. Simoneau & Alison Ciling & Mir M. Khalid & Bharath Sreekumar & Pei-Yi Chen & G. Renuka K, 2022. "Limited cross-variant immunity from SARS-CoV-2 Omicron without vaccination," Nature, Nature, vol. 607(7918), pages 351-355, July.
    3. Qian Wang & Yicheng Guo & Sho Iketani & Manoj S. Nair & Zhiteng Li & Hiroshi Mohri & Maple Wang & Jian Yu & Anthony D. Bowen & Jennifer Y. Chang & Jayesh G. Shah & Nadia Nguyen & Zhiwei Chen & Kathrin, 2022. "Antibody evasion by SARS-CoV-2 Omicron subvariants BA.2.12.1, BA.4 and BA.5," Nature, Nature, vol. 608(7923), pages 603-608, August.
    4. Saranya Sridhar & Roman M. Chicz & William Warren & Jim Tartaglia & Stephen Savarino & Sanjay Gurunathan & Jean-Francois Toussaint, 2022. "The potential of Beta variant containing COVID booster vaccines for chasing Omicron in 2022," Nature Communications, Nature, vol. 13(1), pages 1-3, December.
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