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Reprogramming mechanism dissection and trophoblast replacement application in monkey somatic cell nuclear transfer

Author

Listed:
  • Zhaodi Liao

    (State Key Laboratory of Neuroscience, Chinese Academy of Sciences
    Shanghai Center for Brain Science and Brain-Inspired Technology
    University of Chinese Academy of Sciences)

  • Jixiang Zhang

    (University of Chinese Academy of Sciences
    Institute of Genetics and Developmental Biology, Chinese Academy of Sciences)

  • Shiyu Sun

    (State Key Laboratory of Neuroscience, Chinese Academy of Sciences
    Shanghai Center for Brain Science and Brain-Inspired Technology
    University of Chinese Academy of Sciences)

  • Yuzhuo Li

    (State Key Laboratory of Neuroscience, Chinese Academy of Sciences
    Shanghai Center for Brain Science and Brain-Inspired Technology)

  • Yuting Xu

    (State Key Laboratory of Neuroscience, Chinese Academy of Sciences
    Shanghai Center for Brain Science and Brain-Inspired Technology)

  • Chunyang Li

    (State Key Laboratory of Neuroscience, Chinese Academy of Sciences
    Shanghai Center for Brain Science and Brain-Inspired Technology)

  • Jing Cao

    (State Key Laboratory of Neuroscience, Chinese Academy of Sciences
    Shanghai Center for Brain Science and Brain-Inspired Technology)

  • Yanhong Nie

    (State Key Laboratory of Neuroscience, Chinese Academy of Sciences
    Shanghai Center for Brain Science and Brain-Inspired Technology)

  • Zhuoyue Niu

    (University of Chinese Academy of Sciences
    Institute of Genetics and Developmental Biology, Chinese Academy of Sciences)

  • Jingwen Liu

    (University of Chinese Academy of Sciences
    Institute of Genetics and Developmental Biology, Chinese Academy of Sciences)

  • Falong Lu

    (University of Chinese Academy of Sciences
    Institute of Genetics and Developmental Biology, Chinese Academy of Sciences)

  • Zhen Liu

    (State Key Laboratory of Neuroscience, Chinese Academy of Sciences
    Shanghai Center for Brain Science and Brain-Inspired Technology)

  • Qiang Sun

    (State Key Laboratory of Neuroscience, Chinese Academy of Sciences
    Shanghai Center for Brain Science and Brain-Inspired Technology)

Abstract

Somatic cell nuclear transfer (SCNT) successfully clones cynomolgus monkeys, but the efficiency remains low due to a limited understanding of the reprogramming mechanism. Notably, no rhesus monkey has been cloned through SCNT so far. Our study conducts a comparative analysis of multi-omics datasets, comparing embryos resulting from intracytoplasmic sperm injection (ICSI) with those from SCNT. Our findings reveal a widespread decrease in DNA methylation and the loss of imprinting in maternally imprinted genes within SCNT monkey blastocysts. This loss of imprinting persists in SCNT embryos cultured in-vitro until E17 and in full-term SCNT placentas. Additionally, histological examination of SCNT placentas shows noticeable hyperplasia and calcification. To address these defects, we develop a trophoblast replacement method, ultimately leading to the successful cloning of a healthy male rhesus monkey. These discoveries provide valuable insights into the reprogramming mechanism of monkey SCNT and introduce a promising strategy for primate cloning.

Suggested Citation

  • Zhaodi Liao & Jixiang Zhang & Shiyu Sun & Yuzhuo Li & Yuting Xu & Chunyang Li & Jing Cao & Yanhong Nie & Zhuoyue Niu & Jingwen Liu & Falong Lu & Zhen Liu & Qiang Sun, 2024. "Reprogramming mechanism dissection and trophoblast replacement application in monkey somatic cell nuclear transfer," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:15:y:2024:i:1:d:10.1038_s41467-023-43985-7
    DOI: 10.1038/s41467-023-43985-7
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