IDEAS home Printed from https://ideas.repec.org/a/plo/pone00/0011062.html
   My bibliography  Save this article

The Effect on Intracytoplasmic Sperm Injection Outcome of Genotype, Male Germ Cell Stage and Freeze-Thawing in Mice

Author

Listed:
  • Narumi Ogonuki
  • Manami Mori
  • Akie Shinmen
  • Kimiko Inoue
  • Keiji Mochida
  • Akihiko Ohta
  • Atsuo Ogura

Abstract

Background: Intracytoplasmic sperm injection (ICSI) has been widely used to study the mechanisms of mammalian fertilization and to rescue male-factor infertility in humans and animals. However, very few systematic analyses have been conducted to define factors affecting the efficiency of ICSI. In this study, we undertook a large-scale series of ICSI experiments in mice to define the factors that might affect outcomes. Methodology/Principal Findings: We used a 5×3×2 factorial design with the following factors: mouse genotype (ICR, C57BL/6, DBA/2, C3H/He, and 129/Sv strains), type of male germ cells (epididymal sperm, elongated or round spermatids), and their freeze–thawing treatment. The efficiencies (parameters) of each developmental step were analyzed by three-way ANOVA (significance level P

Suggested Citation

  • Narumi Ogonuki & Manami Mori & Akie Shinmen & Kimiko Inoue & Keiji Mochida & Akihiko Ohta & Atsuo Ogura, 2010. "The Effect on Intracytoplasmic Sperm Injection Outcome of Genotype, Male Germ Cell Stage and Freeze-Thawing in Mice," PLOS ONE, Public Library of Science, vol. 5(6), pages 1-7, June.
    • Handle: RePEc:plo:pone00:0011062
    DOI: 10.1371/journal.pone.0011062
    as

    Download full text from publisher

    File URL: https://journals.plos.org/plosone/article?id=10.1371/journal.pone.0011062
    Download Restriction: no
    File URL: https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0011062&type=printable
    Download Restriction: no
    File URL: https://libkey.io/10.1371/journal.pone.0011062?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. T. Wakayama & A. C. F. Perry & M. Zuccotti & K. R. Johnson & R. Yanagimachi, 1998. "Full-term development of mice from enucleated oocytes injected with cumulus cell nuclei," Nature, Nature, vol. 394(6691), pages 369-374, July.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. M. Samiec & M. Skrzyszowska, 2005. "Microsurgical nuclear transfer by intraooplasmic karyoplast injection as an alternative embryo reconstruction method in somatic cloning of pigs and other mammal species; application value of the metho," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 50(6), pages 235-242.
    2. Ruimin Xu & Qianshu Zhu & Yuyan Zhao & Mo Chen & Lingyue Yang & Shijun Shen & Guang Yang & Zhifei Shi & Xiaolei Zhang & Qi Shi & Xiaochen Kou & Yanhong Zhao & Hong Wang & Cizhong Jiang & Chong Li & Sh, 2023. "Unreprogrammed H3K9me3 prevents minor zygotic genome activation and lineage commitment in SCNT embryos," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Onaizah Onaizah & Liangcheng Xu & Kevin Middleton & Lidan You & Eric Diller, 2020. "Local stimulation of osteocytes using a magnetically actuated oscillating beam," PLOS ONE, Public Library of Science, vol. 15(6), pages 1-15, June.
    4. Zhaodi Liao & Jixiang Zhang & Shiyu Sun & Yuzhuo Li & Yuting Xu & Chunyang Li & Jing Cao & Yanhong Nie & Zhuoyue Niu & Jingwen Liu & Falong Lu & Zhen Liu & Qiang Sun, 2024. "Reprogramming mechanism dissection and trophoblast replacement application in monkey somatic cell nuclear transfer," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    5. Sayaka Wakayama & Daiyu Ito & Erika Hayashi & Takashi Ishiuchi & Teruhiko Wakayama, 2022. "Healthy cloned offspring derived from freeze-dried somatic cells," Nature Communications, Nature, vol. 13(1), pages 1-9, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:plo:pone00:0011062. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: plosone (email available below). General contact details of provider: https://journals.plos.org/plosone/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.