IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-43239-6.html
   My bibliography  Save this article

Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs

Author

Listed:
  • Ye He

    (Duke University)

  • Shujie Yang

    (Duke University)

  • Pengzhan Liu

    (Duke University)

  • Ke Li

    (Duke University)

  • Ke Jin

    (Duke University)

  • Ryan Becker

    (Duke University)

  • Jinxin Zhang

    (Duke University)

  • Chuanchuan Lin

    (Xinqiao Hospital)

  • Jianping Xia

    (Duke University)

  • Zhehan Ma

    (Duke University)

  • Zhiteng Ma

    (Duke University)

  • Ruoyu Zhong

    (Duke University)

  • Luke P. Lee

    (Brigham and Women’s Hospital
    University of California, Berkeley
    University of California, Berkeley
    Sungkyunkwan University)

  • Tony Jun Huang

    (Duke University)

Abstract

While mesenchymal stem cells (MSCs) have gained enormous attention due to their unique properties of self-renewal, colony formation, and differentiation potential, the MSC secretome has become attractive due to its roles in immunomodulation, anti-inflammatory activity, angiogenesis, and anti-apoptosis. However, the precise stimulation and efficient production of the MSC secretome for therapeutic applications are challenging problems to solve. Here, we report on Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs: AIMS. We create an acoustofluidic mechanobiological environment to form reproducible three-dimensional MSC aggregates, which produce the MSC secretome with high efficiency. We confirm the increased MSC secretome is due to improved cell-cell interactions using AIMS: the key mediator N-cadherin was up-regulated while functional blocking of N-cadherin resulted in no enhancement of the secretome. After being primed by IFN-γ, the secretome profile of the MSC aggregates contains more anti-inflammatory cytokines and can be used to inhibit the pro-inflammatory response of M1 phenotype macrophages, suppress T cell activation, and support B cell functions. As such, the MSC secretome can be modified for personalized secretome-based therapies. AIMS acts as a powerful tool for improving the MSC secretome and precisely tuning the secretory profile to develop new treatments in translational medicine.

Suggested Citation

  • Ye He & Shujie Yang & Pengzhan Liu & Ke Li & Ke Jin & Ryan Becker & Jinxin Zhang & Chuanchuan Lin & Jianping Xia & Zhehan Ma & Zhiteng Ma & Ruoyu Zhong & Luke P. Lee & Tony Jun Huang, 2023. "Acoustofluidic Interfaces for the Mechanobiological Secretome of MSCs," Nature Communications, Nature, vol. 14(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43239-6
    DOI: 10.1038/s41467-023-43239-6
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-43239-6
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-43239-6?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Joseph Rufo & Peiran Zhang & Ruoyu Zhong & Luke P. Lee & Tony Jun Huang, 2022. "A sound approach to advancing healthcare systems: the future of biomedical acoustics," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    2. Michael Baudoin & Jean-Louis Thomas & Roudy Al Sahely & Jean-Claude Gerbedoen & Zhixiong Gong & Aude Sivery & Olivier Bou Matar & Nikolay Smagin & Peter Favreau & Alexis Vlandas, 2020. "Spatially selective manipulation of cells with single-beam acoustical tweezers," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    3. Amy Y. Clark & Karen E. Martin & José R. García & Christopher T. Johnson & Hannah S. Theriault & Woojin M. Han & Dennis W. Zhou & Edward A. Botchwey & Andrés J. García, 2020. "Integrin-specific hydrogels modulate transplanted human bone marrow-derived mesenchymal stem cell survival, engraftment, and reparative activities," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Liang Shen & Zhenhua Tian & Kaichun Yang & Joseph Rich & Jianping Xia & Neil Upreti & Jinxin Zhang & Chuyi Chen & Nanjing Hao & Zhichao Pei & Tony Jun Huang, 2024. "Joint subarray acoustic tweezers enable controllable cell translation, rotation, and deformation," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Alisa H. Isaac & Sarea Y. Recalde Phillips & Elizabeth Ruben & Matthew Estes & Varsha Rajavel & Talia Baig & Carol Paleti & Kirsten Landsgaard & Ryang Hwa Lee & Teja Guda & Michael F. Criscitiello & C, 2024. "Impact of PEG sensitization on the efficacy of PEG hydrogel-mediated tissue engineering," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    2. Liang Shen & Zhenhua Tian & Kaichun Yang & Joseph Rich & Jianping Xia & Neil Upreti & Jinxin Zhang & Chuyi Chen & Nanjing Hao & Zhichao Pei & Tony Jun Huang, 2024. "Joint subarray acoustic tweezers enable controllable cell translation, rotation, and deformation," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    3. Xunwu Hu & Sona Rani Roy & Chengzhi Jin & Guanying Li & Qizheng Zhang & Natsuko Asano & Shunsuke Asahina & Tomoko Kajiwara & Atsushi Takahara & Bolu Feng & Kazuhiro Aoki & Chenjie Xu & Ye Zhang, 2022. "Control cell migration by engineering integrin ligand assembly," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    4. Changdong Chen & Xiao Li & Weimian Li & Ming Xue & Yaoyao Shi & Daxing Dong & Yadong Xu & Youwen Liu & Yangyang Fu, 2024. "Super-resolution acoustic displacement metrology through topological pairs in orbital meta-atoms," Nature Communications, Nature, vol. 15(1), pages 1-8, December.
    5. Jan Durrer & Prajwal Agrawal & Ali Ozgul & Stephan C. F. Neuhauss & Nitesh Nama & Daniel Ahmed, 2022. "A robot-assisted acoustofluidic end effector," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    6. Joseph Rufo & Peiran Zhang & Ruoyu Zhong & Luke P. Lee & Tony Jun Huang, 2022. "A sound approach to advancing healthcare systems: the future of biomedical acoustics," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    7. Mengxi Wu & Zhiteng Ma & Xianchen Xu & Brandon Lu & Yuyang Gu & Janghoon Yoon & Jianping Xia & Zhehan Ma & Neil Upreti & Imran J. Anwar & Stuart J. Knechtle & Eileen T. Chambers & Jean Kwun & Luke P. , 2024. "Acoustofluidic-based therapeutic apheresis system," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43239-6. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.