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Spatial transcriptomics uncover sucrose post-phloem transport during maize kernel development

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  • Yuxin Fu

    (Shanghai Normal University
    Shanghai Institute of Plant Physiology & Ecology
    Sichuan Agricultural University)

  • Wenxin Xiao

    (Shanghai Normal University)

  • Lang Tian

    (Shanghai Normal University)

  • Liangxing Guo

    (Shanghai Institute of Plant Physiology & Ecology)

  • Guangjin Ma

    (Shanghai Institute of Plant Physiology & Ecology
    University of the Chinese Academy of Sciences)

  • Chen Ji

    (Shanghai Institute of Plant Physiology & Ecology
    University of the Chinese Academy of Sciences)

  • Yongcai Huang

    (Shanghai Institute of Plant Physiology & Ecology
    Sichuan Agricultural University)

  • Haihai Wang

    (Shanghai Institute of Plant Physiology & Ecology)

  • Xingguo Wu

    (Shanghai Normal University)

  • Tao Yang

    (Shanghai Institute of Plant Physiology & Ecology
    Sichuan Agricultural University)

  • Jiechen Wang

    (Shanghai Institute of Plant Physiology & Ecology)

  • Jirui Wang

    (Sichuan Agricultural University
    Sichuan Agricultural University)

  • Yongrui Wu

    (Shanghai Institute of Plant Physiology & Ecology)

  • Wenqin Wang

    (Shanghai Normal University)

Abstract

Maize kernels are complex biological systems composed of three genetic sources, namely maternal tissues, progeny embryos, and progeny endosperms. The lack of gene expression profiles with spatial information has limited the understanding of the specific functions of each cell population, and hindered the exploration of superior genes in kernels. In our study, we conduct microscopic sectioning and spatial transcriptomics analysis during the grain filling stage of maize kernels. This enables us to visualize the expression patterns of all genes through electronical RNA in situ hybridization, and identify 11 cell populations and 332 molecular marker genes. Furthermore, we systematically elucidate the spatial storage mechanisms of the three major substances in maize kernels: starch, protein, and oil. These findings provide valuable insights into the functional genes that control agronomic traits in maize kernels.

Suggested Citation

  • Yuxin Fu & Wenxin Xiao & Lang Tian & Liangxing Guo & Guangjin Ma & Chen Ji & Yongcai Huang & Haihai Wang & Xingguo Wu & Tao Yang & Jiechen Wang & Jirui Wang & Yongrui Wu & Wenqin Wang, 2023. "Spatial transcriptomics uncover sucrose post-phloem transport during maize kernel development," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-43006-7
    DOI: 10.1038/s41467-023-43006-7
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    References listed on IDEAS

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    1. Yinping Jiao & Paul Peluso & Jinghua Shi & Tiffany Liang & Michelle C. Stitzer & Bo Wang & Michael S. Campbell & Joshua C. Stein & Xuehong Wei & Chen-Shan Chin & Katherine Guill & Michael Regulski & S, 2017. "Improved maize reference genome with single-molecule technologies," Nature, Nature, vol. 546(7659), pages 524-527, June.
    2. Haihai Wang & Yongcai Huang & Qiao Xiao & Xing Huang & Changsheng Li & Xiaoyan Gao & Qiong Wang & Xiaoli Xiang & Yidong Zhu & Jiechen Wang & Wenqin Wang & Brian A. Larkins & Yongrui Wu, 2020. "Carotenoids modulate kernel texture in maize by influencing amyloplast envelope integrity," Nature Communications, Nature, vol. 11(1), pages 1-13, December.
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