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Multimodal spatiotemporal transcriptomic resolution of embryonic palate osteogenesis

Author

Listed:
  • Jeremie Oliver Piña

    (National Institutes of Health (NIH)
    University of Utah
    University of Maryland)

  • Resmi Raju

    (National Institutes of Health (NIH))

  • Daniela M. Roth

    (National Institutes of Health (NIH)
    University of Alberta)

  • Emma Wentworth Winchester

    (University of Connecticut)

  • Parna Chattaraj

    (National Institutes of Health (NIH))

  • Fahad Kidwai

    (National Institutes of Health (NIH))

  • Fabio R. Faucz

    (National Institutes of Health (NIH))

  • James Iben

    (National Institutes of Health (NIH))

  • Apratim Mitra

    (National Institutes of Health (NIH))

  • Kiersten Campbell

    (National Institutes of Health (NIH))

  • Gus Fridell

    (National Institutes of Health (NIH))

  • Caroline Esnault

    (National Institutes of Health (NIH))

  • Justin L. Cotney

    (University of Connecticut)

  • Ryan K. Dale

    (National Institutes of Health (NIH))

  • Rena N. D’Souza

    (National Institutes of Health (NIH))

Abstract

The terminal differentiation of osteoblasts and subsequent formation of bone marks an important phase in palate development that leads to the separation of the oral and nasal cavities. While the morphogenetic events preceding palatal osteogenesis are well explored, major gaps remain in our understanding of the molecular mechanisms driving the formation of this bony union of the fusing palate. Through bulk, single-nucleus, and spatially resolved RNA-sequencing analyses of the developing secondary palate, we identify a shift in transcriptional programming between embryonic days 14.5 and 15.5 pinpointing the onset of osteogenesis. We define spatially restricted expression patterns of key osteogenic marker genes that are differentially expressed between these developmental timepoints. Finally, we identify genes in the palate highly expressed by palate nasal epithelial cells, also enriched within palatal osteogenic mesenchymal cells. This investigation provides a relevant framework to advance palate-specific diagnostic and therapeutic biomarker discovery.

Suggested Citation

  • Jeremie Oliver Piña & Resmi Raju & Daniela M. Roth & Emma Wentworth Winchester & Parna Chattaraj & Fahad Kidwai & Fabio R. Faucz & James Iben & Apratim Mitra & Kiersten Campbell & Gus Fridell & Caroli, 2023. "Multimodal spatiotemporal transcriptomic resolution of embryonic palate osteogenesis," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-41349-9
    DOI: 10.1038/s41467-023-41349-9
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    References listed on IDEAS

    as
    1. Peng Qiu, 2020. "Embracing the dropouts in single-cell RNA-seq analysis," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    2. Tristan W. Fowler & Troy L. Mitchell & Claudia Y. Janda & Liqin Xie & Shengjiang Tu & Hui Chen & Haili Zhang & Jingjing Ye & Brian Ouyang & Tom Z. Yuan & Sung-Jin Lee & Maureen Newman & Nikita Tripura, 2021. "Development of selective bispecific Wnt mimetics for bone loss and repair," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    3. Karen J. Liu & Joseph R. Arron & Kryn Stankunas & Gerald R. Crabtree & Michael T. Longaker, 2007. "Chemical rescue of cleft palate and midline defects in conditional GSK-3β mice," Nature, Nature, vol. 446(7131), pages 79-82, March.
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