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Super-resolution multicolor fluorescence microscopy enabled by an apochromatic super-oscillatory lens with extended depth-of-focus

Author

Listed:
  • Wenli Li

    (Northwestern Polytechnical University
    Northwestern Polytechnical University
    Northwestern Polytechnical University)

  • Pei He

    (Northwestern Polytechnical University
    Northwestern Polytechnical University
    Northwestern Polytechnical University)

  • Dangyuan Lei

    (City University of Hong Kong)

  • Yulong Fan

    (City University of Hong Kong)

  • Yangtao Du

    (Fudan University)

  • Bo Gao

    (Xi’an Institute of Optics and Precision Mechanics, Chinese Academy of Sciences)

  • Zhiqin Chu

    (The University of Hong Kong)

  • Longqiu Li

    (Harbin Institute of Technology)

  • Kaipeng Liu

    (Harbin Institute of Technology)

  • Chengxu An

    (Northwestern Polytechnical University
    Northwestern Polytechnical University
    Northwestern Polytechnical University)

  • Weizheng Yuan

    (Northwestern Polytechnical University
    Northwestern Polytechnical University
    Northwestern Polytechnical University)

  • Yiting Yu

    (Northwestern Polytechnical University
    Northwestern Polytechnical University
    Northwestern Polytechnical University)

Abstract

Planar super-oscillatory lens (SOL), a far-field subwavelength-focusing diffractive device, holds great potential for achieving sub-diffraction-limit imaging at multiple wavelengths. However, conventional SOL devices suffer from a numerical-aperture-related intrinsic tradeoff among the depth of focus (DoF), chromatic dispersion and focusing spot size. Here, we apply a multi-objective genetic algorithm (GA) optimization approach to design an apochromatic binary-phase SOL having a prolonged DoF, customized working distance (WD), minimized main-lobe size, and suppressed side-lobe intensity. Experimental implementation demonstrates simultaneous focusing of blue, green and red light beams into an optical needle of ~0.5λ in diameter and DOF > 10λ at WD = 428 μm. By integrating this SOL device with a commercial fluorescence microscope, we perform, for the first time, three-dimensional super-resolution multicolor fluorescence imaging of the “unseen” fine structures of neurons. The present study provides not only a practical route to far-field multicolor super-resolution imaging but also a viable approach for constructing imaging systems avoiding complex sample positioning and unfavorable photobleaching.

Suggested Citation

  • Wenli Li & Pei He & Dangyuan Lei & Yulong Fan & Yangtao Du & Bo Gao & Zhiqin Chu & Longqiu Li & Kaipeng Liu & Chengxu An & Weizheng Yuan & Yiting Yu, 2023. "Super-resolution multicolor fluorescence microscopy enabled by an apochromatic super-oscillatory lens with extended depth-of-focus," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40725-9
    DOI: 10.1038/s41467-023-40725-9
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    References listed on IDEAS

    as
    1. Konak, Abdullah & Coit, David W. & Smith, Alice E., 2006. "Multi-objective optimization using genetic algorithms: A tutorial," Reliability Engineering and System Safety, Elsevier, vol. 91(9), pages 992-1007.
    2. Lamiae Abdeladim & Katherine S. Matho & Solène Clavreul & Pierre Mahou & Jean-Marc Sintes & Xavier Solinas & Ignacio Arganda-Carreras & Stephen G. Turney & Jeff W. Lichtman & Anatole Chessel & Alexis-, 2019. "Publisher Correction: Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy," Nature Communications, Nature, vol. 10(1), pages 1-1, December.
    3. Lamiae Abdeladim & Katherine S. Matho & Solène Clavreul & Pierre Mahou & Jean-Marc Sintes & Xavier Solinas & Ignacio Arganda-Carreras & Stephen G. Turney & Jeff W. Lichtman & Anatole Chessel & Alexis-, 2019. "Multicolor multiscale brain imaging with chromatic multiphoton serial microscopy," Nature Communications, Nature, vol. 10(1), pages 1-14, December.
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