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Cryopreservation and revival of Hawaiian stony corals using isochoric vitrification

Author

Listed:
  • Matthew J. Powell-Palm

    (Texas A&M University
    Texas A&M University)

  • E. Michael Henley

    (Smithsonian National Zoo and Conservation Biology Institute
    University of Hawaiʻi at Mānoa)

  • Anthony N. Consiglio

    (University of California Berkeley)

  • Claire Lager

    (Smithsonian National Zoo and Conservation Biology Institute
    University of Hawaiʻi at Mānoa)

  • Brooke Chang

    (University of California Berkeley)

  • Riley Perry

    (Smithsonian National Zoo and Conservation Biology Institute
    University of Hawaiʻi at Mānoa)

  • Kendall Fitzgerald

    (Smithsonian National Zoo and Conservation Biology Institute)

  • Jonathan Daly

    (Taronga Institute of Science and Learning, Taronga Conservation Society Australia
    University of New South Wales)

  • Boris Rubinsky

    (University of California Berkeley)

  • Mary Hagedorn

    (Smithsonian National Zoo and Conservation Biology Institute
    University of Hawaiʻi at Mānoa)

Abstract

Corals are under siege by both local and global threats, creating a worldwide reef crisis. Cryopreservation is an important intervention measure and a vital component of the modern coral conservation toolkit, but preservation techniques are currently limited to sensitive reproductive materials that can only be obtained a few nights per year during spawning. Here, we report the successful cryopreservation and revival of cm-scale coral fragments via mL-scale isochoric vitrification. We demonstrate coral viability at 24 h post-thaw using a calibrated oxygen-uptake respirometry technique, and further show that the method can be applied in a passive, electronics-free configuration. Finally, we detail a complete prototype coral cryopreservation pipeline, which provides a platform for essential next steps in modulating post-thaw stress and initiating long-term growth. These findings pave the way towards an approach that can be rapidly deployed around the world to secure the biological genetic diversity of our vanishing coral reefs.

Suggested Citation

  • Matthew J. Powell-Palm & E. Michael Henley & Anthony N. Consiglio & Claire Lager & Brooke Chang & Riley Perry & Kendall Fitzgerald & Jonathan Daly & Boris Rubinsky & Mary Hagedorn, 2023. "Cryopreservation and revival of Hawaiian stony corals using isochoric vitrification," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40500-w
    DOI: 10.1038/s41467-023-40500-w
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    References listed on IDEAS

    as
    1. Haishui Huang & Martin L. Yarmush & O. Berk Usta, 2018. "Long-term deep-supercooling of large-volume water and red cell suspensions via surface sealing with immiscible liquids," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    2. Li Zhan & Min-gang Li & Thomas Hays & John Bischof, 2021. "Cryopreservation method for Drosophila melanogaster embryos," Nature Communications, Nature, vol. 12(1), pages 1-10, December.
    3. D. R. Bellwood & T. P. Hughes & C. Folke & M. Nyström, 2004. "Confronting the coral reef crisis," Nature, Nature, vol. 429(6994), pages 827-833, June.
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    Cited by:

    1. Arian Zarriz & Baptiste Journaux & Matthew J. Powell-Palm, 2024. "On the equilibrium limit of liquid stability in pressurized aqueous systems," Nature Communications, Nature, vol. 15(1), pages 1-10, December.

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