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Control of motor landing and processivity by the CAP-Gly domain in the KIF13B tail

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  • Xiangyu Fan

    (University of California - Davis)

  • Richard J. McKenney

    (University of California - Davis)

Abstract

Microtubules are major components of the eukaryotic cytoskeleton. Posttranslational modifications (PTMs) of tubulin regulates interactions with microtubule-associated proteins (MAPs). One unique PTM is the cyclical removal and re-addition of the C-terminal tyrosine of α-tubulin and MAPs containing CAP-Gly domains specifically recognize tyrosinated microtubules. KIF13B, a long-distance transport kinesin, contains a conserved CAP-Gly domain, but the role of the CAP-Gly domain in KIF13B’s motility along microtubules remains unknown. To address this, we investigate the interaction between KIF13B’s CAP-Gly domain, and tyrosinated microtubules. We find that KIF13B’s CAP-Gly domain influences the initial motor-microtubule interaction, as well as processive motility along microtubules. The effect of the CAP-Gly domain is enhanced when the motor domain is in the ADP state, suggesting an interplay between the N-terminal motor domain and C-terminal CAP-Gly domain. These results reveal that specialized kinesin tail domains play active roles in the initiation and continuation of motor movement.

Suggested Citation

  • Xiangyu Fan & Richard J. McKenney, 2023. "Control of motor landing and processivity by the CAP-Gly domain in the KIF13B tail," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-40425-4
    DOI: 10.1038/s41467-023-40425-4
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    References listed on IDEAS

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    1. Wenjuan Wang & Jinqi Ren & Weiye Song & Yong Zhang & Wei Feng, 2022. "The architecture of kinesin-3 KLP-6 reveals a multilevel-lockdown mechanism for autoinhibition," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    2. Peter Bieling & Liedewij Laan & Henry Schek & E. Laura Munteanu & Linda Sandblad & Marileen Dogterom & Damian Brunner & Thomas Surrey, 2007. "Reconstitution of a microtubule plus-end tracking system in vitro," Nature, Nature, vol. 450(7172), pages 1100-1105, December.
    3. Rong Liu & Neil Billington & Yi Yang & Charles Bond & Amy Hong & Verl Siththanandan & Yasuharu Takagi & James R. Sellers, 2021. "A binding protein regulates myosin-7a dimerization and actin bundle assembly," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
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