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Reconstitution of a microtubule plus-end tracking system in vitro

Author

Listed:
  • Peter Bieling

    (European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany)

  • Liedewij Laan

    (FOM Institute for Atomic and Molecular Physics (AMOLF), Kruislaan 407, 1098 SJ Amsterdam, The Netherlands)

  • Henry Schek

    (European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany)

  • E. Laura Munteanu

    (FOM Institute for Atomic and Molecular Physics (AMOLF), Kruislaan 407, 1098 SJ Amsterdam, The Netherlands)

  • Linda Sandblad

    (European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany)

  • Marileen Dogterom

    (FOM Institute for Atomic and Molecular Physics (AMOLF), Kruislaan 407, 1098 SJ Amsterdam, The Netherlands)

  • Damian Brunner

    (European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany)

  • Thomas Surrey

    (European Molecular Biology Laboratory, Cell Biology and Biophysics Unit, Meyerhofstrasse 1, 69117 Heidelberg, Germany)

Abstract

A combination of novel surface chemistry and fluorescence microscopy allows visualization of dynamic microtubules and plus-end tracking proteins in real time. Three microtubule binding proteins from fission yeast in the in vitro reconstitution system are used, and the results presented yield new insights into the mechanism of plus-end binding proteins in microtubule dynamics.

Suggested Citation

  • Peter Bieling & Liedewij Laan & Henry Schek & E. Laura Munteanu & Linda Sandblad & Marileen Dogterom & Damian Brunner & Thomas Surrey, 2007. "Reconstitution of a microtubule plus-end tracking system in vitro," Nature, Nature, vol. 450(7172), pages 1100-1105, December.
    • Handle: RePEc:nat:nature:v:450:y:2007:i:7172:d:10.1038_nature06386
    DOI: 10.1038/nature06386
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    Cited by:

    1. Xiangyu Fan & Richard J. McKenney, 2023. "Control of motor landing and processivity by the CAP-Gly domain in the KIF13B tail," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    2. Pengge Qian & Xu Wang & Cuirong Guan & Xin Fang & Mengya Cai & Chuan-qi Zhong & Yong Cui & Yanbin Li & Luming Yao & Huiting Cui & Kai Jiang & Jing Yuan, 2022. "Apical anchorage and stabilization of subpellicular microtubules by apical polar ring ensures Plasmodium ookinete infection in mosquito," Nature Communications, Nature, vol. 13(1), pages 1-23, December.
    3. Xi Wang & Fan Zheng & Yuan-yuan Yi & Gao-yuan Wang & Li-xin Hong & Dannel McCollum & Chuanhai Fu & Yamei Wang & Quan-wen Jin, 2022. "Ubiquitination of CLIP-170 family protein restrains polarized growth upon DNA replication stress," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

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