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Structural journey of an insecticidal protein against western corn rootworm

Author

Listed:
  • Guendalina Marini

    (University of London
    Centre for Structural Systems Biology (CSSB), Leibniz-Institut für Virologie (LIV), Universitätsklinikum Hamburg-Eppendorf (UKE))

  • Brad Poland

    (Corteva Agriscience)

  • Chris Leininger

    (Corteva Agriscience
    Syngenta)

  • Natalya Lukoyanova

    (University of London)

  • Dan Spielbauer

    (Corteva Agriscience)

  • Jennifer K. Barry

    (Corteva Agriscience)

  • Dan Altier

    (Corteva Agriscience)

  • Amy Lum

    (Corteva Agriscience
    Willow Biosciences)

  • Eric Scolaro

    (Corteva Agriscience)

  • Claudia Pérez Ortega

    (Corteva Agriscience
    Hologic, Inc.)

  • Nasser Yalpani

    (Corteva Agriscience
    University of British Columbia Okanagan)

  • Gary Sandahl

    (Corteva Agriscience)

  • Tim Mabry

    (Corteva Agriscience)

  • Jeffrey Klever

    (Corteva Agriscience)

  • Timothy Nowatzki

    (Corteva Agriscience)

  • Jian-Zhou Zhao

    (Corteva Agriscience)

  • Amit Sethi

    (Corteva Agriscience)

  • Adane Kassa

    (Corteva Agriscience)

  • Virginia Crane

    (Corteva Agriscience)

  • Albert L. Lu

    (Corteva Agriscience)

  • Mark E. Nelson

    (Corteva Agriscience)

  • Narayanan Eswar

    (Corteva Agriscience)

  • Maya Topf

    (University of London
    Centre for Structural Systems Biology (CSSB), Leibniz-Institut für Virologie (LIV), Universitätsklinikum Hamburg-Eppendorf (UKE))

  • Helen R. Saibil

    (University of London)

Abstract

The broad adoption of transgenic crops has revolutionized agriculture. However, resistance to insecticidal proteins by agricultural pests poses a continuous challenge to maintaining crop productivity and new proteins are urgently needed to replace those utilized for existing transgenic traits. We identified an insecticidal membrane attack complex/perforin (MACPF) protein, Mpf2Ba1, with strong activity against the devastating coleopteran pest western corn rootworm (WCR) and a novel site of action. Using an integrative structural biology approach, we determined monomeric, pre-pore and pore structures, revealing changes between structural states at high resolution. We discovered an assembly inhibition mechanism, a molecular switch that activates pre-pore oligomerization upon gut fluid incubation and solved the highest resolution MACPF pore structure to-date. Our findings demonstrate not only the utility of Mpf2Ba1 in the development of biotechnology solutions for protecting maize from WCR to promote food security, but also uncover previously unknown mechanistic principles of bacterial MACPF assembly.

Suggested Citation

  • Guendalina Marini & Brad Poland & Chris Leininger & Natalya Lukoyanova & Dan Spielbauer & Jennifer K. Barry & Dan Altier & Amy Lum & Eric Scolaro & Claudia Pérez Ortega & Nasser Yalpani & Gary Sandahl, 2023. "Structural journey of an insecticidal protein against western corn rootworm," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39891-7
    DOI: 10.1038/s41467-023-39891-7
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    References listed on IDEAS

    as
    1. Kathryn Tunyasuvunakool & Jonas Adler & Zachary Wu & Tim Green & Michal Zielinski & Augustin Žídek & Alex Bridgland & Andrew Cowie & Clemens Meyer & Agata Laydon & Sameer Velankar & Gerard J. Kleywegt, 2021. "Highly accurate protein structure prediction for the human proteome," Nature, Nature, vol. 596(7873), pages 590-596, August.
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