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The ER calcium channel Csg2 integrates sphingolipid metabolism with autophagy

Author

Listed:
  • Shiyan Liu

    (Sichuan University)

  • Mutian Chen

    (Sichuan University
    Sichuan University
    City of Future Medicine)

  • Yichang Wang

    (Sichuan University)

  • Yuqing Lei

    (West China Second University Hospital, Sichuan University)

  • Ting Huang

    (Sichuan University)

  • Yabin Zhang

    (Sichuan University)

  • Sin Man Lam

    (Chinese Academy of Sciences
    LipidALL Technologies Company Limited)

  • Huihui Li

    (West China Second University Hospital, Sichuan University)

  • Shiqian Qi

    (Sichuan University)

  • Jia Geng

    (Sichuan University
    Sichuan University
    City of Future Medicine)

  • Kefeng Lu

    (Sichuan University)

Abstract

Sphingolipids are ubiquitous components of membranes and function as bioactive lipid signaling molecules. Here, through genetic screening and lipidomics analyses, we find that the endoplasmic reticulum (ER) calcium channel Csg2 integrates sphingolipid metabolism with autophagy by regulating ER calcium homeostasis in the yeast Saccharomyces cerevisiae. Csg2 functions as a calcium release channel and maintains calcium homeostasis in the ER, which enables normal functioning of the essential sphingolipid synthase Aur1. Under starvation conditions, deletion of Csg2 causes increases in calcium levels in the ER and then disturbs Aur1 stability, leading to accumulation of the bioactive sphingolipid phytosphingosine, which specifically and completely blocks autophagy and induces loss of starvation resistance in cells. Our findings indicate that calcium homeostasis in the ER mediated by the channel Csg2 translates sphingolipid metabolism into autophagy regulation, further supporting the role of the ER as a signaling hub for calcium homeostasis, sphingolipid metabolism and autophagy.

Suggested Citation

  • Shiyan Liu & Mutian Chen & Yichang Wang & Yuqing Lei & Ting Huang & Yabin Zhang & Sin Man Lam & Huihui Li & Shiqian Qi & Jia Geng & Kefeng Lu, 2023. "The ER calcium channel Csg2 integrates sphingolipid metabolism with autophagy," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-39482-6
    DOI: 10.1038/s41467-023-39482-6
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    1. Ke Sun & Changjian Zhao & Xiaojun Zeng & Yuejia Chen & Xin Jiang & Xianting Ding & Lu Gou & Haiyang Xie & Xinqiong Li & Xialin Zhang & Sheng Lin & Linqin Dou & Long Wei & Haofu Niu & Ming Zhang & Ruoc, 2019. "Active DNA unwinding and transport by a membrane-adapted helicase nanopore," Nature Communications, Nature, vol. 10(1), pages 1-11, December.
    2. Akiko Kuma & Masahiko Hatano & Makoto Matsui & Akitsugu Yamamoto & Haruaki Nakaya & Tamotsu Yoshimori & Yoshinori Ohsumi & Takeshi Tokuhisa & Noboru Mizushima, 2004. "The role of autophagy during the early neonatal starvation period," Nature, Nature, vol. 432(7020), pages 1032-1036, December.
    3. Xuan Li & Xiao-Qi Wu & Rong Deng & Dan-Dan Li & Jun Tang & Wen-Dan Chen & Jing-Hong Chen & Jiao Ji & Lin Jiao & Shan Jiang & Fen Yang & Gong-Kan Feng & Ravichandran Senthilkumar & Fei Yue & Hai-Liang , 2017. "CaMKII-mediated Beclin 1 phosphorylation regulates autophagy that promotes degradation of Id and neuroblastoma cell differentiation," Nature Communications, Nature, vol. 8(1), pages 1-16, December.
    4. Suneng Fu & Ling Yang & Ping Li & Oliver Hofmann & Lee Dicker & Winston Hide & Xihong Lin & Steven M. Watkins & Alexander R. Ivanov & Gökhan S. Hotamisligil, 2011. "Aberrant lipid metabolism disrupts calcium homeostasis causing liver endoplasmic reticulum stress in obesity," Nature, Nature, vol. 473(7348), pages 528-531, May.
    5. Hwan-Woo Park & Haeli Park & Ian A. Semple & Insook Jang & Seung-Hyun Ro & Myungjin Kim & Victor A. Cazares & Edward L. Stuenkel & Jung-Jae Kim & Jeong Sig Kim & Jun Hee Lee, 2014. "Pharmacological correction of obesity-induced autophagy arrest using calcium channel blockers," Nature Communications, Nature, vol. 5(1), pages 1-12, December.
    6. Heeseon An & Alban Ordureau & Maria Körner & Joao A. Paulo & J. Wade Harper, 2020. "Systematic quantitative analysis of ribosome inventory during nutrient stress," Nature, Nature, vol. 583(7815), pages 303-309, July.
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