IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-38066-8.html
   My bibliography  Save this article

Cationic crosslinked carbon dots-adjuvanted intranasal vaccine induces protective immunity against Omicron-included SARS-CoV-2 variants

Author

Listed:
  • Hong Lei

    (West China Hospital, Sichuan University)

  • Aqu Alu

    (West China Hospital, Sichuan University)

  • Jingyun Yang

    (West China Hospital, Sichuan University)

  • Xi He

    (West China Hospital, Sichuan University)

  • Cai He

    (West China Hospital, Sichuan University)

  • Wenyan Ren

    (West China Hospital, Sichuan University)

  • Zimin Chen

    (West China Hospital, Sichuan University)

  • Weiqi Hong

    (West China Hospital, Sichuan University)

  • Li Chen

    (West China Hospital, Sichuan University)

  • Xuemei He

    (West China Hospital, Sichuan University)

  • Li Yang

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

  • Jiong Li

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

  • Zhenling Wang

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

  • Wei Wang

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

  • Yuquan Wei

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

  • Shuaiyao Lu

    (Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Guangwen Lu

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

  • Xiangrong Song

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

  • Xiawei Wei

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

Abstract

Mucosal immunity plays a significant role in the first-line defense against viruses transmitted and infected through the respiratory system, such as SARS-CoV-2. However, the lack of effective and safe adjuvants currently limits the development of COVID-19 mucosal vaccines. In the current study, we prepare an intranasal vaccine containing cationic crosslinked carbon dots (CCD) and a SARS-CoV-2 antigen, RBD-HR with spontaneous antigen particlization. Intranasal immunization with CCD/RBD-HR induces high levels of antibodies with broad-spectrum neutralization against authentic viruses/pseudoviruses of Omicron-included variants and protects immunized female BALB/c mice from Omicron infection. Despite strong systemic cellular immune response stimulation, the intranasal CCD/RBD-HR vaccine also induces potent mucosal immunity as determined by the generation of tissue-resident T cells in the lungs and airway. Moreover, CCD/RBD-HR not only activates professional antigen-presenting cells (APCs), dendritic cells, but also effectively targets nasal epithelial cells, promotes antigen binding via sialic acid, and surprisingly provokes the antigen-presenting of nasal epithelial cells. We demonstrate that CCD is a promising intranasal vaccine adjuvant for provoking strong mucosal immunity and might be a candidate adjuvant for intranasal vaccine development for many types of infectious diseases, including COVID-19.

Suggested Citation

  • Hong Lei & Aqu Alu & Jingyun Yang & Xi He & Cai He & Wenyan Ren & Zimin Chen & Weiqi Hong & Li Chen & Xuemei He & Li Yang & Jiong Li & Zhenling Wang & Wei Wang & Yuquan Wei & Shuaiyao Lu & Guangwen Lu, 2023. "Cationic crosslinked carbon dots-adjuvanted intranasal vaccine induces protective immunity against Omicron-included SARS-CoV-2 variants," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38066-8
    DOI: 10.1038/s41467-023-38066-8
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-38066-8
    File Function: Abstract
    Download Restriction: no

    File URL: https://libkey.io/10.1038/s41467-023-38066-8?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Anukul T. Shenoy & Carolina Lyon De Ana & Emad I. Arafa & Isabelle Salwig & Kimberly A. Barker & Filiz T. Korkmaz & Aditya Ramanujan & Neelou S. Etesami & Alicia M. Soucy & Ian M. C. Martin & Brian R., 2021. "Antigen presentation by lung epithelial cells directs CD4+ TRM cell function and regulates barrier immunity," Nature Communications, Nature, vol. 12(1), pages 1-16, December.
    2. Emily Waltz, 2022. "How nasal-spray vaccines could change the pandemic," Nature, Nature, vol. 609(7926), pages 240-242, September.
    3. Jingyun Yang & Wei Wang & Zimin Chen & Shuaiyao Lu & Fanli Yang & Zhenfei Bi & Linlin Bao & Fei Mo & Xue Li & Yong Huang & Weiqi Hong & Yun Yang & Yuan Zhao & Fei Ye & Sheng Lin & Wei Deng & Hua Chen , 2020. "A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity," Nature, Nature, vol. 586(7830), pages 572-577, October.
    4. Christie Aschwanden, 2021. "Five reasons why COVID herd immunity is probably impossible," Nature, Nature, vol. 591(7851), pages 520-522, March.
    5. Dennis Lapuente & Jana Fuchs & Jonas Willar & Ana Vieira Antão & Valentina Eberlein & Nadja Uhlig & Leila Issmail & Anna Schmidt & Friederike Oltmanns & Antonia Sophia Peter & Sandra Mueller-Schmucker, 2021. "Protective mucosal immunity against SARS-CoV-2 after heterologous systemic prime-mucosal boost immunization," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    6. Cai He & Jingyun Yang & Weiqi Hong & Zimin Chen & Dandan Peng & Hong Lei & Aqu Alu & Xuemei He & Zhenfei Bi & Xiaohua Jiang & Guowen Jia & Yun Yang & Yanan Zhou & Wenhai Yu & Cong Tang & Qing Huang & , 2022. "A self-assembled trimeric protein vaccine induces protective immunity against Omicron variant," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    7. Rogan A. Grant & Luisa Morales-Nebreda & Nikolay S. Markov & Suchitra Swaminathan & Melissa Querrey & Estefany R. Guzman & Darryl A. Abbott & Helen K. Donnelly & Alvaro Donayre & Isaac A. Goldberg & Z, 2021. "Circuits between infected macrophages and T cells in SARS-CoV-2 pneumonia," Nature, Nature, vol. 590(7847), pages 635-641, February.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Aloysious Ssemaganda & Huong Mai Nguyen & Faisal Nuhu & Naima Jahan & Catherine M. Card & Sandra Kiazyk & Giulia Severini & Yoav Keynan & Ruey-Chyi Su & Hezhao Ji & Bernard Abrenica & Paul J. McLaren , 2022. "Expansion of cytotoxic tissue-resident CD8+ T cells and CCR6+CD161+ CD4+ T cells in the nasal mucosa following mRNA COVID-19 vaccination," Nature Communications, Nature, vol. 13(1), pages 1-9, December.
    2. Chung-Wei Kuo, 2021. "Can We Return to Our Normal Life When the Pandemic Is under Control? A Preliminary Study on the Influence of COVID-19 on the Tourism Characteristics of Taiwan," Sustainability, MDPI, vol. 13(17), pages 1-17, August.
    3. Dapeng Li & David R. Martinez & Alexandra Schäfer & Haiyan Chen & Maggie Barr & Laura L. Sutherland & Esther Lee & Robert Parks & Dieter Mielke & Whitney Edwards & Amanda Newman & Kevin W. Bock & Mahn, 2022. "Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    4. Zheng Fang & Hongqiang Qin & Jiawei Mao & Zhongyu Wang & Na Zhang & Yan Wang & Luyao Liu & Yongzhan Nie & Mingming Dong & Mingliang Ye, 2022. "Glyco-Decipher enables glycan database-independent peptide matching and in-depth characterization of site-specific N-glycosylation," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    5. Wanbo Tai & Shengyong Feng & Benjie Chai & Shuaiyao Lu & Guangyu Zhao & Dong Chen & Wenhai Yu & Liting Ren & Huicheng Shi & Jing Lu & Zhuming Cai & Mujia Pang & Xu Tan & Penghua Wang & Jinzhong Lin & , 2023. "An mRNA-based T-cell-inducing antigen strengthens COVID-19 vaccine against SARS-CoV-2 variants," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    6. Anneliese S. Ashhurst & Matt D. Johansen & Joshua W. C. Maxwell & Skye Stockdale & Caroline L. Ashley & Anupriya Aggarwal & Rezwan Siddiquee & Stefan Miemczyk & Duc H. Nguyen & Joel P. Mackay & Claudi, 2022. "Mucosal TLR2-activating protein-based vaccination induces potent pulmonary immunity and protection against SARS-CoV-2 in mice," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    7. Jessica Carrick-Hagenbarth & Eric Edlund & Avanti Mukherjee, 2023. "Analysis of Hybrid Epidemiological-Economic Models of COVID-19 Mitigation Policies," Eastern Economic Journal, Palgrave Macmillan;Eastern Economic Association, vol. 49(4), pages 585-612, October.
    8. Balisacan, Arsenio M. & dela Cruz, Russel Matthew M., 2021. "When a Pandemic Strikes: Balancing Health and Economy toward Sustainable and Inclusive Recovery," MPRA Paper 111259, University Library of Munich, Germany.
    9. Rhys Hamon & Miranda P. Ween, 2022. "E-Cigarette Vapour Increases ACE2 and TMPRSS2 Expression in a Flavour- and Nicotine-Dependent Manner," IJERPH, MDPI, vol. 19(22), pages 1-12, November.
    10. Nazeem Muhajarine & Daniel A Adeyinka & Jessica McCutcheon & Kathryn L Green & Miles Fahlman & Natalie Kallio, 2021. "COVID-19 vaccine hesitancy and refusal and associated factors in an adult population in Saskatchewan, Canada: Evidence from predictive modelling," PLOS ONE, Public Library of Science, vol. 16(11), pages 1-18, November.
    11. Xiaolei Pei & Li Liu & Jieru Wang & Changyuan Guo & Qingqing Li & Jia Li & Qian Ren & Runzhi Ma & Yi Zheng & Yan Zhang & Li Liu & Danfeng Zheng & Pingzhang Wang & Ping Jiang & Xiaoming Feng & Erlie Ji, 2024. "Exosomal secreted SCIMP regulates communication between macrophages and neutrophils in pneumonia," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    12. Mario Coccia, 2023. "COVID-19 Vaccination is not a Sufficient Public Policy to face Crisis Management of next Pandemic Threats," Public Organization Review, Springer, vol. 23(4), pages 1353-1367, December.
    13. Rachel Erickson & Chang Huang & Cameron Allen & Joanna Ireland & Gwynne Roth & Zhongcheng Zou & Jinghua Lu & Bernard A. P. Lafont & Nicole L. Garza & Beniah Brumbaugh & Ming Zhao & Motoshi Suzuki & Li, 2023. "SARS-CoV-2 infection of human lung epithelial cells induces TMPRSS-mediated acute fibrin deposition," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    14. Cai He & Jingyun Yang & Weiqi Hong & Zimin Chen & Dandan Peng & Hong Lei & Aqu Alu & Xuemei He & Zhenfei Bi & Xiaohua Jiang & Guowen Jia & Yun Yang & Yanan Zhou & Wenhai Yu & Cong Tang & Qing Huang & , 2022. "A self-assembled trimeric protein vaccine induces protective immunity against Omicron variant," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    15. Leila R. Martins & Lina Sieverling & Michelle Michelhans & Chiara Schiller & Cihan Erkut & Thomas G. P. Grünewald & Sergio Triana & Stefan Fröhling & Lars Velten & Hanno Glimm & Claudia Scholl, 2024. "Single-cell division tracing and transcriptomics reveal cell types and differentiation paths in the regenerating lung," Nature Communications, Nature, vol. 15(1), pages 1-20, December.
    16. Suellen Hopfer & Emilia J. Fields & Magdalen Ramirez & Sorina Neang Long & Heather C. Huszti & Adrijana Gombosev & Bernadette Boden-Albala & Dara H. Sorkin & Dan M. Cooper, 2022. "Adolescent COVID-19 Vaccine Decision-Making among Parents in Southern California," IJERPH, MDPI, vol. 19(7), pages 1-20, April.
    17. Erick Armingol & Hratch M. Baghdassarian & Cameron Martino & Araceli Perez-Lopez & Caitlin Aamodt & Rob Knight & Nathan E. Lewis, 2022. "Context-aware deconvolution of cell–cell communication with Tensor-cell2cell," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    18. Tom Chang & Mireille Jacobson & Manisha Shah & Rajiv Pramanik & Samir B. Shah, 2021. "Financial Incentives and Other Nudges Do Not Increase COVID-19 Vaccinations among the Vaccine Hesitant," NBER Working Papers 29403, National Bureau of Economic Research, Inc.
    19. Andrea Toth & Paranthaman Kannan & John Snowball & Matthew Kofron & Joseph A. Wayman & James P. Bridges & Emily R. Miraldi & Daniel Swarr & William J. Zacharias, 2023. "Alveolar epithelial progenitor cells require Nkx2-1 to maintain progenitor-specific epigenomic state during lung homeostasis and regeneration," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    20. Ramy Shaaban & Ramy Mohamed Ghazy & Fawzia Elsherif & Nancy Ali & Youssef Yakoub & Maged Osama Aly & Rony ElMakhzangy & Marwa Shawky Abdou & Bonny McKinna & Amira Mohamed Elzorkany & Fatimah Abdullah , 2022. "COVID-19 Vaccine Acceptance among Social Media Users: A Content Analysis, Multi-Continent Study," IJERPH, MDPI, vol. 19(9), pages 1-14, May.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38066-8. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.