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Cationic crosslinked carbon dots-adjuvanted intranasal vaccine induces protective immunity against Omicron-included SARS-CoV-2 variants

Author

Listed:
  • Hong Lei

    (West China Hospital, Sichuan University)

  • Aqu Alu

    (West China Hospital, Sichuan University)

  • Jingyun Yang

    (West China Hospital, Sichuan University)

  • Xi He

    (West China Hospital, Sichuan University)

  • Cai He

    (West China Hospital, Sichuan University)

  • Wenyan Ren

    (West China Hospital, Sichuan University)

  • Zimin Chen

    (West China Hospital, Sichuan University)

  • Weiqi Hong

    (West China Hospital, Sichuan University)

  • Li Chen

    (West China Hospital, Sichuan University)

  • Xuemei He

    (West China Hospital, Sichuan University)

  • Li Yang

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

  • Jiong Li

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

  • Zhenling Wang

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

  • Wei Wang

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

  • Yuquan Wei

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

  • Shuaiyao Lu

    (Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Guangwen Lu

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

  • Xiangrong Song

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

  • Xiawei Wei

    (West China Hospital, Sichuan University
    WestVac Biopharma Co. Ltd.)

Abstract

Mucosal immunity plays a significant role in the first-line defense against viruses transmitted and infected through the respiratory system, such as SARS-CoV-2. However, the lack of effective and safe adjuvants currently limits the development of COVID-19 mucosal vaccines. In the current study, we prepare an intranasal vaccine containing cationic crosslinked carbon dots (CCD) and a SARS-CoV-2 antigen, RBD-HR with spontaneous antigen particlization. Intranasal immunization with CCD/RBD-HR induces high levels of antibodies with broad-spectrum neutralization against authentic viruses/pseudoviruses of Omicron-included variants and protects immunized female BALB/c mice from Omicron infection. Despite strong systemic cellular immune response stimulation, the intranasal CCD/RBD-HR vaccine also induces potent mucosal immunity as determined by the generation of tissue-resident T cells in the lungs and airway. Moreover, CCD/RBD-HR not only activates professional antigen-presenting cells (APCs), dendritic cells, but also effectively targets nasal epithelial cells, promotes antigen binding via sialic acid, and surprisingly provokes the antigen-presenting of nasal epithelial cells. We demonstrate that CCD is a promising intranasal vaccine adjuvant for provoking strong mucosal immunity and might be a candidate adjuvant for intranasal vaccine development for many types of infectious diseases, including COVID-19.

Suggested Citation

  • Hong Lei & Aqu Alu & Jingyun Yang & Xi He & Cai He & Wenyan Ren & Zimin Chen & Weiqi Hong & Li Chen & Xuemei He & Li Yang & Jiong Li & Zhenling Wang & Wei Wang & Yuquan Wei & Shuaiyao Lu & Guangwen Lu, 2023. "Cationic crosslinked carbon dots-adjuvanted intranasal vaccine induces protective immunity against Omicron-included SARS-CoV-2 variants," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
  • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-38066-8
    DOI: 10.1038/s41467-023-38066-8
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