Author
Listed:
- Jingyun Yang
(Sichuan University)
- Wei Wang
(Sichuan University)
- Zimin Chen
(Sichuan University)
- Shuaiyao Lu
(Chinese Academy of Medical Sciences and Peking Union Medical College)
- Fanli Yang
(Sichuan University)
- Zhenfei Bi
(Sichuan University)
- Linlin Bao
(Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)
- Fei Mo
(Sichuan University)
- Xue Li
(Sichuan University)
- Yong Huang
(Sichuan University)
- Weiqi Hong
(Sichuan University)
- Yun Yang
(Chinese Academy of Medical Sciences and Peking Union Medical College)
- Yuan Zhao
(Chinese Academy of Medical Sciences and Peking Union Medical College)
- Fei Ye
(Sichuan University)
- Sheng Lin
(Sichuan University)
- Wei Deng
(Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)
- Hua Chen
(Sichuan University)
- Hong Lei
(Sichuan University)
- Ziqi Zhang
(Sichuan University)
- Min Luo
(Sichuan University)
- Hong Gao
(Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)
- Yue Zheng
(Sichuan University)
- Yanqiu Gong
(Sichuan University)
- Xiaohua Jiang
(Sichuan University)
- Yanfeng Xu
(Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)
- Qi Lv
(Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)
- Dan Li
(Sichuan University)
- Manni Wang
(Sichuan University)
- Fengdi Li
(Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)
- Shunyi Wang
(Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)
- Guanpeng Wang
(Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)
- Pin Yu
(Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)
- Yajin Qu
(Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)
- Li Yang
(Sichuan University)
- Hongxin Deng
(Sichuan University)
- Aiping Tong
(Sichuan University)
- Jiong Li
(Sichuan University)
- Zhenling Wang
(Sichuan University)
- Jinliang Yang
(Sichuan University)
- Guobo Shen
(Sichuan University)
- Zhiwei Zhao
(Sichuan University)
- Yuhua Li
(National Institutes for Food and Drug Control (NIFDC))
- Jingwen Luo
(Sichuan University)
- Hongqi Liu
(Chinese Academy of Medical Sciences and Peking Union Medical College)
- Wenhai Yu
(Chinese Academy of Medical Sciences and Peking Union Medical College)
- Mengli Yang
(Chinese Academy of Medical Sciences and Peking Union Medical College)
- Jingwen Xu
(Chinese Academy of Medical Sciences and Peking Union Medical College)
- Junbin Wang
(Chinese Academy of Medical Sciences and Peking Union Medical College)
- Haiyan Li
(Chinese Academy of Medical Sciences and Peking Union Medical College)
- Haixuan Wang
(Chinese Academy of Medical Sciences and Peking Union Medical College)
- Dexuan Kuang
(Chinese Academy of Medical Sciences and Peking Union Medical College)
- Panpan Lin
(Sichuan University)
- Zhengtao Hu
(State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center for Biotherapy)
- Wei Guo
(State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center for Biotherapy)
- Wei Cheng
(Sichuan University)
- Yanlin He
(State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center for Biotherapy)
- Xiangrong Song
(Sichuan University)
- Chong Chen
(Sichuan University)
- Zhihong Xue
(Sichuan University)
- Shaohua Yao
(Sichuan University)
- Lu Chen
(Sichuan University)
- Xuelei Ma
(Sichuan University)
- Siyuan Chen
(Sichuan University)
- Maling Gou
(Sichuan University)
- Weijin Huang
(National Institutes for Food and Drug Control (NIFDC))
- Youchun Wang
(National Institutes for Food and Drug Control (NIFDC))
- Changfa Fan
(National Institutes for Food and Drug Control (NIFDC))
- Zhixin Tian
(Tongji University)
- Ming Shi
(National Clinical Research Center for Infectious Diseases)
- Fu-Sheng Wang
(National Clinical Research Center for Infectious Diseases)
- Lunzhi Dai
(Sichuan University)
- Min Wu
(Sichuan University)
- Gen Li
(Macau University of Science and Technology)
- Guangyu Wang
(Tsinghua University)
- Yong Peng
(Sichuan University)
- Zhiyong Qian
(Sichuan University)
- Canhua Huang
(Sichuan University)
- Johnson Yiu-Nam Lau
(Hong Kong Polytechnic University)
- Zhenglin Yang
(University of Electronic Science and Technology of China)
- Yuquan Wei
(Sichuan University)
- Xiaobo Cen
(Sichuan University
State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center for Biotherapy)
- Xiaozhong Peng
(Chinese Academy of Medical Sciences and Peking Union Medical College
School of Basic Medicine, Peking Union Medical College)
- Chuan Qin
(Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)
- Kang Zhang
(Macau University of Science and Technology)
- Guangwen Lu
(Sichuan University
Sichuan University)
- Xiawei Wei
(Sichuan University)
Abstract
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes a respiratory disease called coronavirus disease 2019 (COVID-19), the spread of which has led to a pandemic. An effective preventive vaccine against this virus is urgently needed. As an essential step during infection, SARS-CoV-2 uses the receptor-binding domain (RBD) of the spike protein to engage with the receptor angiotensin-converting enzyme 2 (ACE2) on host cells1,2. Here we show that a recombinant vaccine that comprises residues 319–545 of the RBD of the spike protein induces a potent functional antibody response in immunized mice, rabbits and non-human primates (Macaca mulatta) as early as 7 or 14 days after the injection of a single vaccine dose. The sera from the immunized animals blocked the binding of the RBD to ACE2, which is expressed on the cell surface, and neutralized infection with a SARS-CoV-2 pseudovirus and live SARS-CoV-2 in vitro. Notably, vaccination also provided protection in non-human primates to an in vivo challenge with SARS-CoV-2. We found increased levels of RBD-specific antibodies in the sera of patients with COVID-19. We show that several immune pathways and CD4 T lymphocytes are involved in the induction of the vaccine antibody response. Our findings highlight the importance of the RBD domain in the design of SARS-CoV-2 vaccines and provide a rationale for the development of a protective vaccine through the induction of antibodies against the RBD domain.
Suggested Citation
Jingyun Yang & Wei Wang & Zimin Chen & Shuaiyao Lu & Fanli Yang & Zhenfei Bi & Linlin Bao & Fei Mo & Xue Li & Yong Huang & Weiqi Hong & Yun Yang & Yuan Zhao & Fei Ye & Sheng Lin & Wei Deng & Hua Chen , 2020.
"A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity,"
Nature, Nature, vol. 586(7830), pages 572-577, October.
Handle:
RePEc:nat:nature:v:586:y:2020:i:7830:d:10.1038_s41586-020-2599-8
DOI: 10.1038/s41586-020-2599-8
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Citations
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Cited by:
- Zheng Fang & Hongqiang Qin & Jiawei Mao & Zhongyu Wang & Na Zhang & Yan Wang & Luyao Liu & Yongzhan Nie & Mingming Dong & Mingliang Ye, 2022.
"Glyco-Decipher enables glycan database-independent peptide matching and in-depth characterization of site-specific N-glycosylation,"
Nature Communications, Nature, vol. 13(1), pages 1-15, December.
- Cai He & Jingyun Yang & Weiqi Hong & Zimin Chen & Dandan Peng & Hong Lei & Aqu Alu & Xuemei He & Zhenfei Bi & Xiaohua Jiang & Guowen Jia & Yun Yang & Yanan Zhou & Wenhai Yu & Cong Tang & Qing Huang & , 2022.
"A self-assembled trimeric protein vaccine induces protective immunity against Omicron variant,"
Nature Communications, Nature, vol. 13(1), pages 1-14, December.
- Wanbo Tai & Shengyong Feng & Benjie Chai & Shuaiyao Lu & Guangyu Zhao & Dong Chen & Wenhai Yu & Liting Ren & Huicheng Shi & Jing Lu & Zhuming Cai & Mujia Pang & Xu Tan & Penghua Wang & Jinzhong Lin & , 2023.
"An mRNA-based T-cell-inducing antigen strengthens COVID-19 vaccine against SARS-CoV-2 variants,"
Nature Communications, Nature, vol. 14(1), pages 1-15, December.
- Hong Lei & Aqu Alu & Jingyun Yang & Xi He & Cai He & Wenyan Ren & Zimin Chen & Weiqi Hong & Li Chen & Xuemei He & Li Yang & Jiong Li & Zhenling Wang & Wei Wang & Yuquan Wei & Shuaiyao Lu & Guangwen Lu, 2023.
"Cationic crosslinked carbon dots-adjuvanted intranasal vaccine induces protective immunity against Omicron-included SARS-CoV-2 variants,"
Nature Communications, Nature, vol. 14(1), pages 1-17, December.
- Qingqing Feng & Keman Cheng & Lizhuo Zhang & Dongshu Wang & Xiaoyu Gao & Jie Liang & Guangna Liu & Nana Ma & Chen Xu & Ming Tang & Liting Chen & Xinwei Wang & Xuehui Ma & Jiajia Zou & Quanwei Shi & Pe, 2024.
"Rationally designed multimeric nanovaccines using icosahedral DNA origami for display of SARS-CoV-2 receptor binding domain,"
Nature Communications, Nature, vol. 15(1), pages 1-17, December.
- James Logue & Robert M. Johnson & Nita Patel & Bin Zhou & Sonia Maciejewski & Bryant Foreman & Haixia Zhou & Alyse D. Portnoff & Jing-Hui Tian & Asma Rehman & Marisa E. McGrath & Robert E. Haupt & Stu, 2023.
"Immunogenicity and protection of a variant nanoparticle vaccine that confers broad neutralization against SARS-CoV-2 variants,"
Nature Communications, Nature, vol. 14(1), pages 1-16, December.
- Dapeng Li & David R. Martinez & Alexandra Schäfer & Haiyan Chen & Maggie Barr & Laura L. Sutherland & Esther Lee & Robert Parks & Dieter Mielke & Whitney Edwards & Amanda Newman & Kevin W. Bock & Mahn, 2022.
"Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine,"
Nature Communications, Nature, vol. 13(1), pages 1-15, December.
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