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A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity

Author

Listed:
  • Jingyun Yang

    (Sichuan University)

  • Wei Wang

    (Sichuan University)

  • Zimin Chen

    (Sichuan University)

  • Shuaiyao Lu

    (Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Fanli Yang

    (Sichuan University)

  • Zhenfei Bi

    (Sichuan University)

  • Linlin Bao

    (Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)

  • Fei Mo

    (Sichuan University)

  • Xue Li

    (Sichuan University)

  • Yong Huang

    (Sichuan University)

  • Weiqi Hong

    (Sichuan University)

  • Yun Yang

    (Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Yuan Zhao

    (Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Fei Ye

    (Sichuan University)

  • Sheng Lin

    (Sichuan University)

  • Wei Deng

    (Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)

  • Hua Chen

    (Sichuan University)

  • Hong Lei

    (Sichuan University)

  • Ziqi Zhang

    (Sichuan University)

  • Min Luo

    (Sichuan University)

  • Hong Gao

    (Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)

  • Yue Zheng

    (Sichuan University)

  • Yanqiu Gong

    (Sichuan University)

  • Xiaohua Jiang

    (Sichuan University)

  • Yanfeng Xu

    (Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)

  • Qi Lv

    (Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)

  • Dan Li

    (Sichuan University)

  • Manni Wang

    (Sichuan University)

  • Fengdi Li

    (Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)

  • Shunyi Wang

    (Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)

  • Guanpeng Wang

    (Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)

  • Pin Yu

    (Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)

  • Yajin Qu

    (Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)

  • Li Yang

    (Sichuan University)

  • Hongxin Deng

    (Sichuan University)

  • Aiping Tong

    (Sichuan University)

  • Jiong Li

    (Sichuan University)

  • Zhenling Wang

    (Sichuan University)

  • Jinliang Yang

    (Sichuan University)

  • Guobo Shen

    (Sichuan University)

  • Zhiwei Zhao

    (Sichuan University)

  • Yuhua Li

    (National Institutes for Food and Drug Control (NIFDC))

  • Jingwen Luo

    (Sichuan University)

  • Hongqi Liu

    (Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Wenhai Yu

    (Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Mengli Yang

    (Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Jingwen Xu

    (Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Junbin Wang

    (Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Haiyan Li

    (Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Haixuan Wang

    (Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Dexuan Kuang

    (Chinese Academy of Medical Sciences and Peking Union Medical College)

  • Panpan Lin

    (Sichuan University)

  • Zhengtao Hu

    (State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center for Biotherapy)

  • Wei Guo

    (State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center for Biotherapy)

  • Wei Cheng

    (Sichuan University)

  • Yanlin He

    (State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center for Biotherapy)

  • Xiangrong Song

    (Sichuan University)

  • Chong Chen

    (Sichuan University)

  • Zhihong Xue

    (Sichuan University)

  • Shaohua Yao

    (Sichuan University)

  • Lu Chen

    (Sichuan University)

  • Xuelei Ma

    (Sichuan University)

  • Siyuan Chen

    (Sichuan University)

  • Maling Gou

    (Sichuan University)

  • Weijin Huang

    (National Institutes for Food and Drug Control (NIFDC))

  • Youchun Wang

    (National Institutes for Food and Drug Control (NIFDC))

  • Changfa Fan

    (National Institutes for Food and Drug Control (NIFDC))

  • Zhixin Tian

    (Tongji University)

  • Ming Shi

    (National Clinical Research Center for Infectious Diseases)

  • Fu-Sheng Wang

    (National Clinical Research Center for Infectious Diseases)

  • Lunzhi Dai

    (Sichuan University)

  • Min Wu

    (Sichuan University)

  • Gen Li

    (Macau University of Science and Technology)

  • Guangyu Wang

    (Tsinghua University)

  • Yong Peng

    (Sichuan University)

  • Zhiyong Qian

    (Sichuan University)

  • Canhua Huang

    (Sichuan University)

  • Johnson Yiu-Nam Lau

    (Hong Kong Polytechnic University)

  • Zhenglin Yang

    (University of Electronic Science and Technology of China)

  • Yuquan Wei

    (Sichuan University)

  • Xiaobo Cen

    (Sichuan University
    State Key Laboratory of Biotherapy and Cancer Center, West China Hospital, Sichuan University, Collaborative Innovation Center for Biotherapy)

  • Xiaozhong Peng

    (Chinese Academy of Medical Sciences and Peking Union Medical College
    School of Basic Medicine, Peking Union Medical College)

  • Chuan Qin

    (Institute of Laboratory Animal Science, Chinese Academy of Medical Sciences and Comparative Medicine Center, Peking Union Medical College)

  • Kang Zhang

    (Macau University of Science and Technology)

  • Guangwen Lu

    (Sichuan University
    Sichuan University)

  • Xiawei Wei

    (Sichuan University)

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) causes a respiratory disease called coronavirus disease 2019 (COVID-19), the spread of which has led to a pandemic. An effective preventive vaccine against this virus is urgently needed. As an essential step during infection, SARS-CoV-2 uses the receptor-binding domain (RBD) of the spike protein to engage with the receptor angiotensin-converting enzyme 2 (ACE2) on host cells1,2. Here we show that a recombinant vaccine that comprises residues 319–545 of the RBD of the spike protein induces a potent functional antibody response in immunized mice, rabbits and non-human primates (Macaca mulatta) as early as 7 or 14 days after the injection of a single vaccine dose. The sera from the immunized animals blocked the binding of the RBD to ACE2, which is expressed on the cell surface, and neutralized infection with a SARS-CoV-2 pseudovirus and live SARS-CoV-2 in vitro. Notably, vaccination also provided protection in non-human primates to an in vivo challenge with SARS-CoV-2. We found increased levels of RBD-specific antibodies in the sera of patients with COVID-19. We show that several immune pathways and CD4 T lymphocytes are involved in the induction of the vaccine antibody response. Our findings highlight the importance of the RBD domain in the design of SARS-CoV-2 vaccines and provide a rationale for the development of a protective vaccine through the induction of antibodies against the RBD domain.

Suggested Citation

  • Jingyun Yang & Wei Wang & Zimin Chen & Shuaiyao Lu & Fanli Yang & Zhenfei Bi & Linlin Bao & Fei Mo & Xue Li & Yong Huang & Weiqi Hong & Yun Yang & Yuan Zhao & Fei Ye & Sheng Lin & Wei Deng & Hua Chen , 2020. "A vaccine targeting the RBD of the S protein of SARS-CoV-2 induces protective immunity," Nature, Nature, vol. 586(7830), pages 572-577, October.
  • Handle: RePEc:nat:nature:v:586:y:2020:i:7830:d:10.1038_s41586-020-2599-8
    DOI: 10.1038/s41586-020-2599-8
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    Citations

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    Cited by:

    1. Zheng Fang & Hongqiang Qin & Jiawei Mao & Zhongyu Wang & Na Zhang & Yan Wang & Luyao Liu & Yongzhan Nie & Mingming Dong & Mingliang Ye, 2022. "Glyco-Decipher enables glycan database-independent peptide matching and in-depth characterization of site-specific N-glycosylation," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    2. Cai He & Jingyun Yang & Weiqi Hong & Zimin Chen & Dandan Peng & Hong Lei & Aqu Alu & Xuemei He & Zhenfei Bi & Xiaohua Jiang & Guowen Jia & Yun Yang & Yanan Zhou & Wenhai Yu & Cong Tang & Qing Huang & , 2022. "A self-assembled trimeric protein vaccine induces protective immunity against Omicron variant," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    3. Wanbo Tai & Shengyong Feng & Benjie Chai & Shuaiyao Lu & Guangyu Zhao & Dong Chen & Wenhai Yu & Liting Ren & Huicheng Shi & Jing Lu & Zhuming Cai & Mujia Pang & Xu Tan & Penghua Wang & Jinzhong Lin & , 2023. "An mRNA-based T-cell-inducing antigen strengthens COVID-19 vaccine against SARS-CoV-2 variants," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    4. Hong Lei & Aqu Alu & Jingyun Yang & Xi He & Cai He & Wenyan Ren & Zimin Chen & Weiqi Hong & Li Chen & Xuemei He & Li Yang & Jiong Li & Zhenling Wang & Wei Wang & Yuquan Wei & Shuaiyao Lu & Guangwen Lu, 2023. "Cationic crosslinked carbon dots-adjuvanted intranasal vaccine induces protective immunity against Omicron-included SARS-CoV-2 variants," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    5. James Logue & Robert M. Johnson & Nita Patel & Bin Zhou & Sonia Maciejewski & Bryant Foreman & Haixia Zhou & Alyse D. Portnoff & Jing-Hui Tian & Asma Rehman & Marisa E. McGrath & Robert E. Haupt & Stu, 2023. "Immunogenicity and protection of a variant nanoparticle vaccine that confers broad neutralization against SARS-CoV-2 variants," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    6. Dapeng Li & David R. Martinez & Alexandra Schäfer & Haiyan Chen & Maggie Barr & Laura L. Sutherland & Esther Lee & Robert Parks & Dieter Mielke & Whitney Edwards & Amanda Newman & Kevin W. Bock & Mahn, 2022. "Breadth of SARS-CoV-2 neutralization and protection induced by a nanoparticle vaccine," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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