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In mitosis integrins reduce adhesion to extracellular matrix and strengthen adhesion to adjacent cells

Author

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  • Maximilian Huber

    (Eidgenössische Technische Hochschule (ETH) Zurich)

  • Javier Casares-Arias

    (Eidgenössische Technische Hochschule (ETH) Zurich)

  • Reinhard Fässler

    (Max Planck Institute of Biochemistry)

  • Daniel J. Müller

    (Eidgenössische Technische Hochschule (ETH) Zurich)

  • Nico Strohmeyer

    (Eidgenössische Technische Hochschule (ETH) Zurich)

Abstract

To enter mitosis, most adherent animal cells reduce adhesion, which is followed by cell rounding. How mitotic cells regulate adhesion to neighboring cells and extracellular matrix (ECM) proteins is poorly understood. Here we report that, similar to interphase, mitotic cells can employ integrins to initiate adhesion to the ECM in a kindlin- and talin-dependent manner. However, unlike interphase cells, we find that mitotic cells cannot engage newly bound integrins to actomyosin via talin or vinculin to reinforce adhesion. We show that the missing actin connection of newly bound integrins leads to transient ECM-binding and prevents cell spreading during mitosis. Furthermore, β1 integrins strengthen the adhesion of mitotic cells to adjacent cells, which is supported by vinculin, kindlin, and talin1. We conclude that this dual role of integrins in mitosis weakens the cell-ECM adhesion and strengthens the cell-cell adhesion to prevent delamination of the rounding and dividing cell.

Suggested Citation

  • Maximilian Huber & Javier Casares-Arias & Reinhard Fässler & Daniel J. Müller & Nico Strohmeyer, 2023. "In mitosis integrins reduce adhesion to extracellular matrix and strengthen adhesion to adjacent cells," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37760-x
    DOI: 10.1038/s41467-023-37760-x
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    References listed on IDEAS

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    1. Mark Pogson & Cristina Parola & William J. Kelton & Paul Heuberger & Sai T. Reddy, 2016. "Immunogenomic engineering of a plug-and-(dis)play hybridoma platform," Nature Communications, Nature, vol. 7(1), pages 1-10, November.
    2. Martin P. Stewart & Jonne Helenius & Yusuke Toyoda & Subramanian P. Ramanathan & Daniel J. Muller & Anthony A. Hyman, 2011. "Hydrostatic pressure and the actomyosin cortex drive mitotic cell rounding," Nature, Nature, vol. 469(7329), pages 226-230, January.
    3. Mingxi Yao & Benjamin T. Goult & Benjamin Klapholz & Xian Hu & Christopher P. Toseland & Yingjian Guo & Peiwen Cong & Michael P. Sheetz & Jie Yan, 2016. "The mechanical response of talin," Nature Communications, Nature, vol. 7(1), pages 1-11, September.
    4. Barbara Sorce & Carlos Escobedo & Yusuke Toyoda & Martin P. Stewart & Cedric J. Cattin & Richard Newton & Indranil Banerjee & Alexander Stettler & Botond Roska & Suzanne Eaton & Anthony A. Hyman & And, 2015. "Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial confinement," Nature Communications, Nature, vol. 6(1), pages 1-12, December.
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    Cited by:

    1. Sophie Herzog & Gotthold Fläschner & Ilaria Incaviglia & Javier Casares Arias & Aaron Ponti & Nico Strohmeyer & Michele M. Nava & Daniel J. Müller, 2024. "Monitoring the mass, eigenfrequency, and quality factor of mammalian cells," Nature Communications, Nature, vol. 15(1), pages 1-13, December.

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