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Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial confinement

Author

Listed:
  • Barbara Sorce

    (Eidgenössische Technische Hochschule (ETH) Zurich)

  • Carlos Escobedo

    (Eidgenössische Technische Hochschule (ETH) Zurich
    Queen’s University)

  • Yusuke Toyoda

    (Max Planck Institute of Molecular Cell Biology and Genetics)

  • Martin P. Stewart

    (Eidgenössische Technische Hochschule (ETH) Zurich
    Massachusetts Institute of Technology (MIT)
    The David H. Koch Institute for Integrative Cancer Research)

  • Cedric J. Cattin

    (Eidgenössische Technische Hochschule (ETH) Zurich)

  • Richard Newton

    (Eidgenössische Technische Hochschule (ETH) Zurich)

  • Indranil Banerjee

    (Neural Circuit Laboratories, Friedrich Miescher Institute (FMI) for Biomedical Research)

  • Alexander Stettler

    (Eidgenössische Technische Hochschule (ETH) Zurich)

  • Botond Roska

    (Neural Circuit Laboratories, Friedrich Miescher Institute (FMI) for Biomedical Research)

  • Suzanne Eaton

    (Max Planck Institute of Molecular Cell Biology and Genetics)

  • Anthony A. Hyman

    (Max Planck Institute of Molecular Cell Biology and Genetics)

  • Andreas Hierlemann

    (Eidgenössische Technische Hochschule (ETH) Zurich)

  • Daniel J. Müller

    (Eidgenössische Technische Hochschule (ETH) Zurich)

Abstract

Little is known about how mitotic cells round against epithelial confinement. Here, we engineer micropillar arrays that subject cells to lateral mechanical confinement similar to that experienced in epithelia. If generating sufficient force to deform the pillars, rounding epithelial (MDCK) cells can create space to divide. However, if mitotic cells cannot create sufficient space, their rounding force, which is generated by actomyosin contraction and hydrostatic pressure, pushes the cell out of confinement. After conducting mitosis in an unperturbed manner, both daughter cells return to the confinement of the pillars. Cells that cannot round against nor escape confinement cannot orient their mitotic spindles and more likely undergo apoptosis. The results highlight how spatially constrained epithelial cells prepare for mitosis: either they are strong enough to round up or they must escape. The ability to escape from confinement and reintegrate after mitosis appears to be a basic property of epithelial cells.

Suggested Citation

  • Barbara Sorce & Carlos Escobedo & Yusuke Toyoda & Martin P. Stewart & Cedric J. Cattin & Richard Newton & Indranil Banerjee & Alexander Stettler & Botond Roska & Suzanne Eaton & Anthony A. Hyman & And, 2015. "Mitotic cells contract actomyosin cortex and generate pressure to round against or escape epithelial confinement," Nature Communications, Nature, vol. 6(1), pages 1-12, December.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms9872
    DOI: 10.1038/ncomms9872
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    Cited by:

    1. Maximilian Huber & Javier Casares-Arias & Reinhard Fässler & Daniel J. Müller & Nico Strohmeyer, 2023. "In mitosis integrins reduce adhesion to extracellular matrix and strengthen adhesion to adjacent cells," Nature Communications, Nature, vol. 14(1), pages 1-17, December.

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