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Phytochrome B photobodies are comprised of phytochrome B and its primary and secondary interacting proteins

Author

Listed:
  • Chanhee Kim

    (Korea Advanced Institute of Science and Technology)

  • Yongmin Kwon

    (Korea Advanced Institute of Science and Technology)

  • Jaehoon Jeong

    (Korea Advanced Institute of Science and Technology)

  • Minji Kang

    (Korea Advanced Institute of Science and Technology)

  • Ga Seul Lee

    (Korea Research Institute of Bioscience and Biotechnology
    Chungbuk National University)

  • Jeong Hee Moon

    (Korea Research Institute of Bioscience and Biotechnology)

  • Hyo-Jun Lee

    (Korea Research Institute of Bioscience and Biotechnology)

  • Youn-Il Park

    (Chungnam National University)

  • Giltsu Choi

    (Korea Advanced Institute of Science and Technology)

Abstract

Phytochrome B (phyB) is a plant photoreceptor that forms a membraneless organelle called a photobody. However, its constituents are not fully known. Here, we isolated phyB photobodies from Arabidopsis leaves using fluorescence-activated particle sorting and analyzed their components. We found that a photobody comprises ~1,500 phyB dimers along with other proteins that could be classified into two groups: The first includes proteins that directly interact with phyB and localize to the photobody when expressed in protoplasts, while the second includes proteins that interact with the first group proteins and require co-expression of a first-group protein to localize to the photobody. As an example of the second group, TOPLESS interacts with PHOTOPERIODIC CONTROL OF HYPOCOTYL 1 (PCH1) and localizes to the photobody when co-expressed with PCH1. Together, our results support that phyB photobodies include not only phyB and its primary interacting proteins but also its secondary interacting proteins.

Suggested Citation

  • Chanhee Kim & Yongmin Kwon & Jaehoon Jeong & Minji Kang & Ga Seul Lee & Jeong Hee Moon & Hyo-Jun Lee & Youn-Il Park & Giltsu Choi, 2023. "Phytochrome B photobodies are comprised of phytochrome B and its primary and secondary interacting proteins," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37421-z
    DOI: 10.1038/s41467-023-37421-z
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