IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v14y2023i1d10.1038_s41467-023-37421-z.html
   My bibliography  Save this article

Phytochrome B photobodies are comprised of phytochrome B and its primary and secondary interacting proteins

Author

Listed:
  • Chanhee Kim

    (Korea Advanced Institute of Science and Technology)

  • Yongmin Kwon

    (Korea Advanced Institute of Science and Technology)

  • Jaehoon Jeong

    (Korea Advanced Institute of Science and Technology)

  • Minji Kang

    (Korea Advanced Institute of Science and Technology)

  • Ga Seul Lee

    (Korea Research Institute of Bioscience and Biotechnology
    Chungbuk National University)

  • Jeong Hee Moon

    (Korea Research Institute of Bioscience and Biotechnology)

  • Hyo-Jun Lee

    (Korea Research Institute of Bioscience and Biotechnology)

  • Youn-Il Park

    (Chungnam National University)

  • Giltsu Choi

    (Korea Advanced Institute of Science and Technology)

Abstract

Phytochrome B (phyB) is a plant photoreceptor that forms a membraneless organelle called a photobody. However, its constituents are not fully known. Here, we isolated phyB photobodies from Arabidopsis leaves using fluorescence-activated particle sorting and analyzed their components. We found that a photobody comprises ~1,500 phyB dimers along with other proteins that could be classified into two groups: The first includes proteins that directly interact with phyB and localize to the photobody when expressed in protoplasts, while the second includes proteins that interact with the first group proteins and require co-expression of a first-group protein to localize to the photobody. As an example of the second group, TOPLESS interacts with PHOTOPERIODIC CONTROL OF HYPOCOTYL 1 (PCH1) and localizes to the photobody when co-expressed with PCH1. Together, our results support that phyB photobodies include not only phyB and its primary interacting proteins but also its secondary interacting proteins.

Suggested Citation

  • Chanhee Kim & Yongmin Kwon & Jaehoon Jeong & Minji Kang & Ga Seul Lee & Jeong Hee Moon & Hyo-Jun Lee & Youn-Il Park & Giltsu Choi, 2023. "Phytochrome B photobodies are comprised of phytochrome B and its primary and secondary interacting proteins," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37421-z
    DOI: 10.1038/s41467-023-37421-z
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-023-37421-z
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-023-37421-z?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Dmitri A. Nusinow & Anne Helfer & Elizabeth E. Hamilton & Jasmine J. King & Takato Imaizumi & Thomas F. Schultz & Eva M. Farré & Steve A. Kay, 2011. "The ELF4–ELF3–LUX complex links the circadian clock to diurnal control of hypocotyl growth," Nature, Nature, vol. 475(7356), pages 398-402, July.
    2. Joseph Hahm & Keunhwa Kim & Yongjian Qiu & Meng Chen, 2020. "Publisher Correction: Increasing ambient temperature progressively disassembles Arabidopsis phytochrome B from individual photobodies with distinct thermostabilities," Nature Communications, Nature, vol. 11(1), pages 1-1, December.
    3. Joseph Hahm & Keunhwa Kim & Yongjian Qiu & Meng Chen, 2020. "Increasing ambient temperature progressively disassembles Arabidopsis phytochrome B from individual photobodies with distinct thermostabilities," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
    4. Qing Liu & Qin Wang & Weixian Deng & Xu Wang & Mingxin Piao & Dawei Cai & Yaxing Li & William D. Barshop & Xiaolan Yu & Tingting Zhou & Bin Liu & Yoshito Oka & James Wohlschlegel & Zecheng Zuo & Chent, 2017. "Molecular basis for blue light-dependent phosphorylation of Arabidopsis cryptochrome 2," Nature Communications, Nature, vol. 8(1), pages 1-12, August.
    5. Chan Yul Yoo & Elise K. Pasoreck & He Wang & Jun Cao & Gregor M. Blaha & Detlef Weigel & Meng Chen, 2019. "Phytochrome activates the plastid-encoded RNA polymerase for chloroplast biogenesis via nucleus-to-plastid signaling," Nature Communications, Nature, vol. 10(1), pages 1-16, December.
    6. Emily J. Yang & Chan Yul Yoo & Jiangxin Liu & He Wang & Jun Cao & Fay-Wei Li & Kathleen M. Pryer & Tai-ping Sun & Detlef Weigel & Pei Zhou & Meng Chen, 2019. "NCP activates chloroplast transcription by controlling phytochrome-dependent dual nuclear and plastidial switches," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    7. Weimin Ni & Shou-Ling Xu & Eduardo González-Grandío & Robert J. Chalkley & Andreas F. R. Huhmer & Alma L. Burlingame & Zhi-Yong Wang & Peter H. Quail, 2017. "PPKs mediate direct signal transfer from phytochrome photoreceptors to transcription factor PIF3," Nature Communications, Nature, vol. 8(1), pages 1-11, August.
    8. Martina Legris & Yetkin Çaka Ince & Christian Fankhauser, 2019. "Molecular mechanisms underlying phytochrome-controlled morphogenesis in plants," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    9. Beatrix Enderle & David J. Sheerin & Inyup Paik & Praveen Kumar Kathare & Philipp Schwenk & Cornelia Klose & Maximilian H. Ulbrich & Enamul Huq & Andreas Hiltbrunner, 2017. "PCH1 and PCHL promote photomorphogenesis in plants by controlling phytochrome B dark reversion," Nature Communications, Nature, vol. 8(1), pages 1-9, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Ruth Jean Ae Kim & De Fan & Jiangman He & Keunhwa Kim & Juan Du & Meng Chen, 2024. "Photobody formation spatially segregates two opposing phytochrome B signaling actions of PIF5 degradation and stabilization," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    2. Juan Du & Keunhwa Kim & Meng Chen, 2024. "Distinguishing individual photobodies using Oligopaints reveals thermo-sensitive and -insensitive phytochrome B condensation at distinct subnuclear locations," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    3. Youra Hwang & Soeun Han & Chan Yul Yoo & Liu Hong & Chenjiang You & Brandon H. Le & Hui Shi & Shangwei Zhong & Ute Hoecker & Xuemei Chen & Meng Chen, 2022. "Anterograde signaling controls plastid transcription via sigma factors separately from nuclear photosynthesis genes," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    4. Yuqing He & Yingjun Yu & Xiling Wang & Yumei Qin & Chen Su & Lei Wang, 2022. "Aschoff’s rule on circadian rhythms orchestrated by blue light sensor CRY2 and clock component PRR9," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    5. Zenglin Li & David J. Sheerin & Edda Roepenack-Lahaye & Mark Stahl & Andreas Hiltbrunner, 2022. "The phytochrome interacting proteins ERF55 and ERF58 repress light-induced seed germination in Arabidopsis thaliana," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    6. Yetkin Çaka Ince & Johanna Krahmer & Anne-Sophie Fiorucci & Martine Trevisan & Vinicius Costa Galvão & Leonore Wigger & Sylvain Pradervand & Laetitia Fouillen & Pierre Delft & Manon Genva & Sebastien , 2022. "A combination of plasma membrane sterol biosynthesis and autophagy is required for shade-induced hypocotyl elongation," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    7. Urszula Piskurewicz & Maria Sentandreu & Mayumi Iwasaki & Gaëtan Glauser & Luis Lopez-Molina, 2023. "The Arabidopsis endosperm is a temperature-sensing tissue that implements seed thermoinhibition through phyB," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    8. Weiliang Mo & Junchuan Zhang & Li Zhang & Zhenming Yang & Liang Yang & Nan Yao & Yong Xiao & Tianhong Li & Yaxing Li & Guangmei Zhang & Mingdi Bian & Xinglin Du & Zecheng Zuo, 2022. "Arabidopsis cryptochrome 2 forms photobodies with TCP22 under blue light and regulates the circadian clock," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    9. Maximilian O Press & Amy Lanctot & Christine Queitsch, 2016. "PIF4 and ELF3 Act Independently in Arabidopsis thaliana Thermoresponsive Flowering," PLOS ONE, Public Library of Science, vol. 11(8), pages 1-18, August.
    10. Weixiao Yuan Wahlgren & Elin Claesson & Iida Tuure & Sergio Trillo-Muyo & Szabolcs Bódizs & Janne A. Ihalainen & Heikki Takala & Sebastian Westenhoff, 2022. "Structural mechanism of signal transduction in a phytochrome histidine kinase," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    11. Simone Pieralli, 2019. "Bumper crop or dearth: An economic methodology to identify the disruptive effects of climatic variables on French agriculture [Récolte exceptionnelle ou pénurie : une méthodologie économique pour i," Working Papers hal-02786610, HAL.
    12. Giacomo Salvadori & Veronica Macaluso & Giulia Pellicci & Lorenzo Cupellini & Giovanni Granucci & Benedetta Mennucci, 2022. "Protein control of photochemistry and transient intermediates in phytochromes," Nature Communications, Nature, vol. 13(1), pages 1-10, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37421-z. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.