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PPKs mediate direct signal transfer from phytochrome photoreceptors to transcription factor PIF3

Author

Listed:
  • Weimin Ni

    (University of California
    Plant Gene Expression Center)

  • Shou-Ling Xu

    (University of California
    Carnegie Institution for Science
    Thermo Fisher Scientific)

  • Eduardo González-Grandío

    (University of California
    Plant Gene Expression Center)

  • Robert J. Chalkley

    (University of California)

  • Andreas F. R. Huhmer

    (Thermo Fisher Scientific)

  • Alma L. Burlingame

    (University of California)

  • Zhi-Yong Wang

    (Carnegie Institution for Science)

  • Peter H. Quail

    (University of California
    Plant Gene Expression Center)

Abstract

Upon light-induced nuclear translocation, phytochrome (phy) sensory photoreceptors interact with, and induce rapid phosphorylation and consequent ubiquitin-mediated degradation of, transcription factors, called PIFs, thereby regulating target gene expression and plant development. Nevertheless, the biochemical mechanism of phy-induced PIF phosphorylation has remained ill-defined. Here we identify a family of nuclear protein kinases, designated Photoregulatory Protein Kinases (PPK1–4; formerly called MUT9-Like Kinases (MLKs)), that interact with PIF3 and phyB in a light-induced manner in vivo. Genetic analyses demonstrate that the PPKs are collectively necessary for the normal light-induced phosphorylation and degradation of PIF3. PPK1 directly phosphorylates PIF3 in vitro, with a phosphosite pattern that strongly mimics the light-induced pattern in vivo. These data establish that the PPKs are directly involved in catalysing the photoactivated-phy-induced phosphorylation of PIF3 in vivo, and thereby are critical components of a transcriptionally centred signalling hub that pleiotropically regulates plant growth and development in response to multiple signalling pathways.

Suggested Citation

  • Weimin Ni & Shou-Ling Xu & Eduardo González-Grandío & Robert J. Chalkley & Andreas F. R. Huhmer & Alma L. Burlingame & Zhi-Yong Wang & Peter H. Quail, 2017. "PPKs mediate direct signal transfer from phytochrome photoreceptors to transcription factor PIF3," Nature Communications, Nature, vol. 8(1), pages 1-11, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15236
    DOI: 10.1038/ncomms15236
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    Cited by:

    1. Yuqing He & Yingjun Yu & Xiling Wang & Yumei Qin & Chen Su & Lei Wang, 2022. "Aschoff’s rule on circadian rhythms orchestrated by blue light sensor CRY2 and clock component PRR9," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    2. Chanhee Kim & Yongmin Kwon & Jaehoon Jeong & Minji Kang & Ga Seul Lee & Jeong Hee Moon & Hyo-Jun Lee & Youn-Il Park & Giltsu Choi, 2023. "Phytochrome B photobodies are comprised of phytochrome B and its primary and secondary interacting proteins," Nature Communications, Nature, vol. 14(1), pages 1-13, December.
    3. Youra Hwang & Soeun Han & Chan Yul Yoo & Liu Hong & Chenjiang You & Brandon H. Le & Hui Shi & Shangwei Zhong & Ute Hoecker & Xuemei Chen & Meng Chen, 2022. "Anterograde signaling controls plastid transcription via sigma factors separately from nuclear photosynthesis genes," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    4. Ruth Jean Ae Kim & De Fan & Jiangman He & Keunhwa Kim & Juan Du & Meng Chen, 2024. "Photobody formation spatially segregates two opposing phytochrome B signaling actions of PIF5 degradation and stabilization," Nature Communications, Nature, vol. 15(1), pages 1-14, December.

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