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Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system

Author

Listed:
  • Jinchul Ahn

    (Korea University
    Next&Bio Inc.)

  • Kyungeun Ohk

    (R&D center, Humedix, Co., Ltd.
    Korea University)

  • Jihee Won

    (Korea University
    Next&Bio Inc.)

  • Dong-Hee Choi

    (Korea University
    Next&Bio Inc.)

  • Yong Hun Jung

    (Korea University
    Next&Bio Inc.)

  • Ji Hun Yang

    (Next&Bio Inc.)

  • Yesl Jun

    (Korea University
    Korea Research Institute of Chemical Technology)

  • Jin-A Kim

    (Korea University)

  • Seok Chung

    (Korea University
    Korea University
    Korea Institute of Science and Technology (KIST))

  • Sang-Hoon Lee

    (Korea University
    Korea University)

Abstract

Reconstruction of skin equivalents with physiologically relevant cellular and matrix architecture is indispensable for basic research and industrial applications. As skin-nerve crosstalk is increasingly recognized as a major element of skin physiological pathology, the development of reliable in vitro models to evaluate the selective communication between epidermal keratinocytes and sensory neurons is being demanded. In this study, we present a three-dimensional innervated epidermal keratinocyte layer as a sensory neuron-epidermal keratinocyte co-culture model on a microfluidic chip using the slope-based air-liquid interfacing culture and spatial compartmentalization. Our co-culture model recapitulates a more organized basal-suprabasal stratification, enhanced barrier function, and physiologically relevant anatomical innervation and demonstrated the feasibility of in situ imaging and functional analysis in a cell-type-specific manner, thereby improving the structural and functional limitations of previous coculture models. This system has the potential as an improved surrogate model and platform for biomedical and pharmaceutical research.

Suggested Citation

  • Jinchul Ahn & Kyungeun Ohk & Jihee Won & Dong-Hee Choi & Yong Hun Jung & Ji Hun Yang & Yesl Jun & Jin-A Kim & Seok Chung & Sang-Hoon Lee, 2023. "Modeling of three-dimensional innervated epidermal like-layer in a microfluidic chip-based coculture system," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-37187-4
    DOI: 10.1038/s41467-023-37187-4
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    References listed on IDEAS

    as
    1. Ellen A. Lumpkin & Michael J. Caterina, 2007. "Mechanisms of sensory transduction in the skin," Nature, Nature, vol. 445(7130), pages 858-865, February.
    2. Sheila MacNeil, 2007. "Progress and opportunities for tissue-engineered skin," Nature, Nature, vol. 445(7130), pages 874-880, February.
    Full references (including those not matched with items on IDEAS)

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