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Large-scale identification of genes involved in septal pore plugging in multicellular fungi

Author

Listed:
  • Md. Abdulla Al Mamun

    (The University of Tokyo)

  • Wei Cao

    (National Agriculture and Food Research Organization (NARO))

  • Shugo Nakamura

    (Toyo University)

  • Jun-ichi Maruyama

    (The University of Tokyo
    The University of Tokyo)

Abstract

Multicellular filamentous fungi have septal pores that allow cytoplasmic exchange, and thus connectivity, between neighboring cells in the filament. Hyphal wounding and other stress conditions induce septal pore closure to minimize cytoplasmic loss. However, the composition of the septal pore and the mechanisms underlying its function are not well understood. Here, we set out to identify new septal components by determining the subcellular localization of 776 uncharacterized proteins in a multicellular ascomycete, Aspergillus oryzae. The set of 776 uncharacterized proteins was selected on the basis that their genes were present in the genomes of multicellular, septal pore-bearing ascomycetes (three Aspergillus species, in subdivision Pezizomycotina) and absent/divergent in the genomes of septal pore-lacking ascomycetes (yeasts). Upon determining their subcellular localization, 62 proteins were found to localize to the septum or septal pore. Deletion of the encoding genes revealed that 23 proteins are involved in regulating septal pore plugging upon hyphal wounding. Thus, this study determines the subcellular localization of many uncharacterized proteins in A. oryzae and, in particular, identifies a set of proteins involved in septal pore function.

Suggested Citation

  • Md. Abdulla Al Mamun & Wei Cao & Shugo Nakamura & Jun-ichi Maruyama, 2023. "Large-scale identification of genes involved in septal pore plugging in multicellular fungi," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:14:y:2023:i:1:d:10.1038_s41467-023-36925-y
    DOI: 10.1038/s41467-023-36925-y
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    References listed on IDEAS

    as
    1. Tu Anh Nguyen & Ousmane H. Cissé & Jie Yun Wong & Peng Zheng & David Hewitt & Minou Nowrousian & Jason E. Stajich & Gregory Jedd, 2017. "Innovation and constraint leading to complex multicellularity in the Ascomycota," Nature Communications, Nature, vol. 8(1), pages 1-13, April.
    2. Enikő Kiss & Botond Hegedüs & Máté Virágh & Torda Varga & Zsolt Merényi & Tamás Kószó & Balázs Bálint & Arun N. Prasanna & Krisztina Krizsán & Sándor Kocsubé & Meritxell Riquelme & Norio Takeshita & L, 2019. "Comparative genomics reveals the origin of fungal hyphae and multicellularity," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    3. Won-Ki Huh & James V. Falvo & Luke C. Gerke & Adam S. Carroll & Russell W. Howson & Jonathan S. Weissman & Erin K. O'Shea, 2003. "Global analysis of protein localization in budding yeast," Nature, Nature, vol. 425(6959), pages 686-691, October.
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