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High-frequency synthetic apomixis in hybrid rice

Author

Listed:
  • Aurore Vernet

    (Avenue Agropolis
    CIRAD-INRAE-Institut Agro-University of Montpellier)

  • Donaldo Meynard

    (Avenue Agropolis
    CIRAD-INRAE-Institut Agro-University of Montpellier)

  • Qichao Lian

    (Max Planck Institute for Plant Breeding Research)

  • Delphine Mieulet

    (Avenue Agropolis
    CIRAD-INRAE-Institut Agro-University of Montpellier
    University of Montpellier- IRD-CIRAD)

  • Olivier Gibert

    (Avenue Agropolis
    CIRAD-INRAE-Institut Agro-University of Montpellier
    Rue Jean-François Breton
    Avignon University, CIRAD, Institut Agro, Reunion University)

  • Matilda Bissah

    (CSIR, Plant Genetic Resources Research Institute)

  • Ronan Rivallan

    (Avenue Agropolis
    CIRAD-INRAE-Institut Agro-University of Montpellier)

  • Daphné Autran

    (University of Montpellier- IRD-CIRAD)

  • Olivier Leblanc

    (University of Montpellier- IRD-CIRAD)

  • Anne Cécile Meunier

    (Avenue Agropolis
    CIRAD-INRAE-Institut Agro-University of Montpellier)

  • Julien Frouin

    (Avenue Agropolis
    CIRAD-INRAE-Institut Agro-University of Montpellier)

  • James Taillebois

    (Avenue Agropolis)

  • Kyle Shankle

    (University of California)

  • Imtiyaz Khanday

    (University of California
    University of California)

  • Raphael Mercier

    (Max Planck Institute for Plant Breeding Research)

  • Venkatesan Sundaresan

    (University of California
    University of California
    University of California)

  • Emmanuel Guiderdoni

    (Avenue Agropolis
    CIRAD-INRAE-Institut Agro-University of Montpellier)

Abstract

Introducing asexual reproduction through seeds – apomixis – into crop species could revolutionize agriculture by allowing F1 hybrids with enhanced yield and stability to be clonally propagated. Engineering synthetic apomixis has proven feasible in inbred rice through the inactivation of three genes (MiMe), which results in the conversion of meiosis into mitosis in a line ectopically expressing the BABYBOOM1 (BBM1) parthenogenetic trigger in egg cells. However, only 10–30% of the seeds are clonal. Here, we show that synthetic apomixis can be achieved in an F1 hybrid of rice by inducing MiMe mutations and egg cell expression of BBM1 in a single step. We generate hybrid plants that produce more than 95% of clonal seeds across multiple generations. Clonal apomictic plants maintain the phenotype of the F1 hybrid along successive generations. Our results demonstrate that there is no barrier to almost fully penetrant synthetic apomixis in an important crop species, rendering it compatible with use in agriculture.

Suggested Citation

  • Aurore Vernet & Donaldo Meynard & Qichao Lian & Delphine Mieulet & Olivier Gibert & Matilda Bissah & Ronan Rivallan & Daphné Autran & Olivier Leblanc & Anne Cécile Meunier & Julien Frouin & James Tail, 2022. "High-frequency synthetic apomixis in hybrid rice," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35679-3
    DOI: 10.1038/s41467-022-35679-3
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    References listed on IDEAS

    as
    1. Imtiyaz Khanday & Debra Skinner & Bing Yang & Raphael Mercier & Venkatesan Sundaresan, 2019. "A male-expressed rice embryogenic trigger redirected for asexual propagation through seeds," Nature, Nature, vol. 565(7737), pages 91-95, January.
    2. Graham, Robin, 2002. "A Proposal for IRRI to Establish a Grain Quality and Nutrition Research Center," IRRI Discussion Papers 287611, International Rice Research Institute (IRRI).
    3. Maruthachalam Ravi & Simon W. L. Chan, 2010. "Haploid plants produced by centromere-mediated genome elimination," Nature, Nature, vol. 464(7288), pages 615-618, March.
    4. Xiang Li & Dexuan Meng & Shaojiang Chen & Haishan Luo & Qinghua Zhang & Weiwei Jin & Jianbing Yan, 2017. "Single nucleus sequencing reveals spermatid chromosome fragmentation as a possible cause of maize haploid induction," Nature Communications, Nature, vol. 8(1), pages 1-9, December.
    Full references (including those not matched with items on IDEAS)

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