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Single nucleus sequencing reveals spermatid chromosome fragmentation as a possible cause of maize haploid induction

Author

Listed:
  • Xiang Li

    (Huazhong Agricultural University)

  • Dexuan Meng

    (China Agricultural University)

  • Shaojiang Chen

    (China Agricultural University)

  • Haishan Luo

    (China Agricultural University)

  • Qinghua Zhang

    (Huazhong Agricultural University)

  • Weiwei Jin

    (China Agricultural University)

  • Jianbing Yan

    (Huazhong Agricultural University)

Abstract

Production of maternal haploids using a conspecific haploid inducer is routine and highly efficient in maize. However, the underlying mechanism of haploid induction (HI) is unclear. We develop a method to isolate three nuclei from a pollen grain and four microspores from a tetrad for whole-genome sequencing. A high rate of aneuploidy is observed at the three-nucleus stage (6/22 pollens) rather than at the tetrad stage (1/72 microspores) in one HI line CAU5. Frequent aneuploidy is also observed in another two inducer lines, but not in two regular lines, which implies that HI may be associated with pollen aneuploidy. We further sequence the individual embryos and endosperms of 88 maize kernels crossing between regular and inducer lines. Genome-wide elimination of the CAU5-derived chromosome is identified in eight of 81 embryos. Together, these results suggest that continuous chromosome fragmentation occurring post meiosis in the gametophyte may cause haploidy of the embryo.

Suggested Citation

  • Xiang Li & Dexuan Meng & Shaojiang Chen & Haishan Luo & Qinghua Zhang & Weiwei Jin & Jianbing Yan, 2017. "Single nucleus sequencing reveals spermatid chromosome fragmentation as a possible cause of maize haploid induction," Nature Communications, Nature, vol. 8(1), pages 1-9, December.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_s41467-017-00969-8
    DOI: 10.1038/s41467-017-00969-8
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    Cited by:

    1. Aurore Vernet & Donaldo Meynard & Qichao Lian & Delphine Mieulet & Olivier Gibert & Matilda Bissah & Ronan Rivallan & Daphné Autran & Olivier Leblanc & Anne Cécile Meunier & Julien Frouin & James Tail, 2022. "High-frequency synthetic apomixis in hybrid rice," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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