IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-35064-0.html
   My bibliography  Save this article

The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy

Author

Listed:
  • Alessandro Rossetta

    (Istituto Italiano di Tecnologia
    Istituto Italiano di Tecnologia
    University of Genoa)

  • Eli Slenders

    (Istituto Italiano di Tecnologia)

  • Mattia Donato

    (Istituto Italiano di Tecnologia)

  • Sabrina Zappone

    (Istituto Italiano di Tecnologia
    University of Genoa)

  • Francesco Fersini

    (Istituto Italiano di Tecnologia
    University of Genoa)

  • Martina Bruno

    (Istituto Italiano di Tecnologia
    University of Genoa)

  • Francesco Diotalevi

    (Istituto Italiano di Tecnologia)

  • Luca Lanzanò

    (Istituto Italiano di Tecnologia
    University of Catania)

  • Sami Koho

    (Istituto Italiano di Tecnologia)

  • Giorgio Tortarolo

    (Istituto Italiano di Tecnologia)

  • Andrea Barberis

    (Istituto Italiano di Tecnologia)

  • Marco Crepaldi

    (Istituto Italiano di Tecnologia)

  • Eleonora Perego

    (Istituto Italiano di Tecnologia)

  • Giuseppe Vicidomini

    (Istituto Italiano di Tecnologia)

Abstract

Fluorescence laser-scanning microscopy (LSM) is experiencing a revolution thanks to new single-photon (SP) array detectors, which give access to an entirely new set of single-photon information. Together with the blooming of new SP LSM techniques and the development of tailored SP array detectors, there is a growing need for (i) DAQ systems capable of handling the high-throughput and high-resolution photon information generated by these detectors, and (ii) incorporating these DAQ protocols in existing fluorescence LSMs. We developed an open-source, low-cost, multi-channel time-tagging module (TTM) based on a field-programmable gate array that can tag in parallel multiple single-photon events, with 30 ps precision, and multiple synchronisation events, with 4 ns precision. We use the TTM to demonstrate live-cell super-resolved fluorescence lifetime image scanning microscopy and fluorescence lifetime fluctuation spectroscopy. We expect that our BrightEyes-TTM will support the microscopy community in spreading SP-LSM in many life science laboratories.

Suggested Citation

  • Alessandro Rossetta & Eli Slenders & Mattia Donato & Sabrina Zappone & Francesco Fersini & Martina Bruno & Francesco Diotalevi & Luca Lanzanò & Sami Koho & Giorgio Tortarolo & Andrea Barberis & Marco , 2022. "The BrightEyes-TTM as an open-source time-tagging module for democratising single-photon microscopy," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35064-0
    DOI: 10.1038/s41467-022-35064-0
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-35064-0
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-35064-0?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Brian Owens, 2017. "The microscope makers," Nature, Nature, vol. 551(7682), pages 659-662, November.
    2. Enrica Maria Petrini & Tiziana Ravasenga & Torben J. Hausrat & Giuliano Iurilli & Umberto Olcese & Victor Racine & Jean-Baptiste Sibarita & Tija C. Jacob & Stephen J. Moss & Fabio Benfenati & Paolo Me, 2014. "Synaptic recruitment of gephyrin regulates surface GABAA receptor dynamics for the expression of inhibitory LTP," Nature Communications, Nature, vol. 5(1), pages 1-19, September.
    3. Sami Koho & Giorgio Tortarolo & Marco Castello & Takahiro Deguchi & Alberto Diaspro & Giuseppe Vicidomini, 2019. "Fourier ring correlation simplifies image restoration in fluorescence microscopy," Nature Communications, Nature, vol. 10(1), pages 1-9, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Eleonora Perego & Sabrina Zappone & Francesco Castagnetti & Davide Mariani & Erika Vitiello & Jakob Rupert & Elsa Zacco & Gian Gaetano Tartaglia & Irene Bozzoni & Eli Slenders & Giuseppe Vicidomini, 2023. "Single-photon microscopy to study biomolecular condensates," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Giorgio Tortarolo & Alessandro Zunino & Francesco Fersini & Marco Castello & Simonluca Piazza & Colin J. R. Sheppard & Paolo Bianchini & Alberto Diaspro & Sami Koho & Giuseppe Vicidomini, 2022. "Focus image scanning microscopy for sharp and gentle super-resolved microscopy," Nature Communications, Nature, vol. 13(1), pages 1-14, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Yilun Li & Fang Huang, 2024. "A statistical resolution measure of fluorescence microscopy with finite photons," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    2. Yue Hao & Haowen Liu & Xian-Ting Zeng & Ya Wang & Wan-Xin Zeng & Kang-Ying Qian & Lei Li & Ming-Xuan Chi & Shangbang Gao & Zhitao Hu & Xia-Jing Tong, 2023. "UNC-43/CaMKII-triggered anterograde signals recruit GABAARs to mediate inhibitory synaptic transmission and plasticity at C. elegans NMJs," Nature Communications, Nature, vol. 14(1), pages 1-15, December.
    3. Giorgio Tortarolo & Alessandro Zunino & Francesco Fersini & Marco Castello & Simonluca Piazza & Colin J. R. Sheppard & Paolo Bianchini & Alberto Diaspro & Sami Koho & Giuseppe Vicidomini, 2022. "Focus image scanning microscopy for sharp and gentle super-resolved microscopy," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    4. Anna Rubinski & Noam E Ziv, 2015. "Remodeling and Tenacity of Inhibitory Synapses: Relationships with Network Activity and Neighboring Excitatory Synapses," PLOS Computational Biology, Public Library of Science, vol. 11(11), pages 1-29, November.
    5. Chupao Lin & Juan Santo Domingo Peñaranda & Jolien Dendooven & Christophe Detavernier & David Schaubroeck & Nico Boon & Roel Baets & Nicolas Le Thomas, 2022. "UV photonic integrated circuits for far-field structured illumination autofluorescence microscopy," Nature Communications, Nature, vol. 13(1), pages 1-9, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-35064-0. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.