IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-34581-2.html
   My bibliography  Save this article

Single cell profiling of primary and paired metastatic lymph node tumors in breast cancer patients

Author

Listed:
  • Tong Liu

    (Harbin Medical University Cancer Hospital, Harbin, China; Heilongjiang Academy of Medical Sciences)

  • Cheng Liu

    (Peking University Health Science Center
    Peking University Health Science Center
    Peking University Health Science Center)

  • Meisi Yan

    (Harbin Medical University)

  • Lei Zhang

    (Peking University Health Science Center
    Peking University Health Science Center
    Peking University Health Science Center)

  • Jing Zhang

    (Peking University Health Science Center
    Peking University Health Science Center
    Peking University Health Science Center)

  • Min Xiao

    (Harbin Medical University Cancer Hospital, Harbin, China; Heilongjiang Academy of Medical Sciences)

  • Zhigao Li

    (Harbin Medical University Cancer Hospital, Harbin, China; Heilongjiang Academy of Medical Sciences)

  • Xiaofan Wei

    (Peking University Health Science Center
    Peking University Health Science Center
    Peking University Health Science Center)

  • Hongquan Zhang

    (Peking University Health Science Center
    Peking University Health Science Center
    Peking University Health Science Center
    Shenzhen University School of Medicine)

Abstract

The microenvironment of lymph node metastasized tumors (LNMT) determines tumor progression and response to therapy, but a systematic study of LNMT is lacking. Here, we generate single-cell maps of primary tumors (PTs) and paired LNMTs in 8 breast cancer patients. We demonstrate that the activation, cytotoxicity, and proliferation of T cells are suppressed in LNMT compared with PT. CD4+CXCL13+ T cells in LNMT are more likely to differentiate into an exhausted state. Interestingly, LAMP3+ dendritic cells in LNMT display lower T cell priming and activating ability than in PT. Additionally, we identify a subtype of PLA2G2A+ cancer-associated fibroblasts enriched in HER2+ breast cancer patients that promotes immune infiltration. We also show that the antigen-presentation pathway is downregulated in malignant cells of the metastatic lymph node. Altogether, we characterize the microenvironment of LNMT and PT, which may shed light on the individualized therapeutic strategies for breast cancer patients with lymph node metastasis.

Suggested Citation

  • Tong Liu & Cheng Liu & Meisi Yan & Lei Zhang & Jing Zhang & Min Xiao & Zhigao Li & Xiaofan Wei & Hongquan Zhang, 2022. "Single cell profiling of primary and paired metastatic lymph node tumors in breast cancer patients," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34581-2
    DOI: 10.1038/s41467-022-34581-2
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-34581-2
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-34581-2?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Mihriban Karaayvaz & Simona Cristea & Shawn M. Gillespie & Anoop P. Patel & Ravindra Mylvaganam & Christina C. Luo & Michelle C. Specht & Bradley E. Bernstein & Franziska Michor & Leif W. Ellisen, 2018. "Unravelling subclonal heterogeneity and aggressive disease states in TNBC through single-cell RNA-seq," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    2. Lei Zhang & Xin Yu & Liangtao Zheng & Yuanyuan Zhang & Yansen Li & Qiao Fang & Ranran Gao & Boxi Kang & Qiming Zhang & Julie Y. Huang & Hiroyasu Konno & Xinyi Guo & Yingjiang Ye & Songyuan Gao & Shan , 2018. "Lineage tracking reveals dynamic relationships of T cells in colorectal cancer," Nature, Nature, vol. 564(7735), pages 268-272, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Tingting Liu & Zhenzhen Wang & Xiaotong Xue & Zhe Wang & Yuan Zhang & Zihao Mi & Qing Zhao & Lele Sun & Chuan Wang & Peidian Shi & Gongqi Yu & Meng Wang & Yonghu Sun & Fuzhong Xue & Hong Liu & Furen Z, 2024. "Single-cell transcriptomics analysis of bullous pemphigoid unveils immune-stromal crosstalk in type 2 inflammatory disease," Nature Communications, Nature, vol. 15(1), pages 1-16, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Yanchuan Li & Huamei Li & Cheng Peng & Ge Meng & Yijun Lu & Honglin Liu & Li Cui & Huan Zhou & Zhu Xu & Lingyun Sun & Lihong Liu & Qing Xiong & Beicheng Sun & Shiping Jiao, 2024. "Unraveling the spatial organization and development of human thymocytes through integration of spatial transcriptomics and single-cell multi-omics profiling," Nature Communications, Nature, vol. 15(1), pages 1-25, December.
    2. Keyong Sun & Runda Xu & Fuhai Ma & Naixue Yang & Yang Li & Xiaofeng Sun & Peng Jin & Wenzhe Kang & Lemei Jia & Jianping Xiong & Haitao Hu & Yantao Tian & Xun Lan, 2022. "scRNA-seq of gastric tumor shows complex intercellular interaction with an alternative T cell exhaustion trajectory," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    3. Handong Sun & Lishen Zhang & Zhonglin Wang & Danling Gu & Mengyan Zhu & Yun Cai & Lu Li & Jiaqi Tang & Bin Huang & Bakwatanisa Bosco & Ning Li & Lingxiang Wu & Wei Wu & Liangyu Li & Yuan Liang & Lin L, 2023. "Single-cell transcriptome analysis indicates fatty acid metabolism-mediated metastasis and immunosuppression in male breast cancer," Nature Communications, Nature, vol. 14(1), pages 1-18, December.
    4. Ji-Yong Sung & Hyun-Tae Shin & Kyung-Ah Sohn & Soo-Yong Shin & Woong-Yang Park & Je-Gun Joung, 2019. "Assessment of intratumoral heterogeneity with mutations and gene expression profiles," PLOS ONE, Public Library of Science, vol. 14(7), pages 1-15, July.
    5. Yang Liu & Shuang-Yan Ye & Shuai He & Dong-Mei Chi & Xiu-Zhi Wang & Yue-Feng Wen & Dong Ma & Run-Cong Nie & Pu Xiang & You Zhou & Zhao-Hui Ruan & Rou-Jun Peng & Chun-Ling Luo & Pan-Pan Wei & Guo-Wang , 2024. "Single-cell and spatial transcriptome analyses reveal tertiary lymphoid structures linked to tumour progression and immunotherapy response in nasopharyngeal carcinoma," Nature Communications, Nature, vol. 15(1), pages 1-22, December.
    6. Duy Pham & Xiao Tan & Brad Balderson & Jun Xu & Laura F. Grice & Sohye Yoon & Emily F. Willis & Minh Tran & Pui Yeng Lam & Arti Raghubar & Priyakshi Kalita-de Croft & Sunil Lakhani & Jana Vukovic & Ma, 2023. "Robust mapping of spatiotemporal trajectories and cell–cell interactions in healthy and diseased tissues," Nature Communications, Nature, vol. 14(1), pages 1-25, December.
    7. Sarah Cappuyns & Gino Philips & Vincent Vandecaveye & Bram Boeckx & Rogier Schepers & Thomas Van Brussel & Ingrid Arijs & Aurelie Mechels & Ayse Bassez & Francesca Lodi & Joris Jaekers & Halit Topal &, 2023. "PD-1- CD45RA+ effector-memory CD8 T cells and CXCL10+ macrophages are associated with response to atezolizumab plus bevacizumab in advanced hepatocellular carcinoma," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    8. Ido Nofech-Mozes & David Soave & Philip Awadalla & Sagi Abelson, 2023. "Pan-cancer classification of single cells in the tumour microenvironment," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    9. Qionghua Zhu & Xin Zhao & Yuanhang Zhang & Yanping Li & Shang Liu & Jingxuan Han & Zhiyuan Sun & Chunqing Wang & Daqi Deng & Shanshan Wang & Yisen Tang & Yaling Huang & Siyuan Jiang & Chi Tian & Xi Ch, 2023. "Single cell multi-omics reveal intra-cell-line heterogeneity across human cancer cell lines," Nature Communications, Nature, vol. 14(1), pages 1-21, December.
    10. Xianke Xiang & Yao He & Zemin Zhang & Xuerui Yang, 2024. "Interrogations of single-cell RNA splicing landscapes with SCASL define new cell identities with physiological relevance," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    11. Michele Bortolomeazzi & Lucia Montorsi & Damjan Temelkovski & Mohamed Reda Keddar & Amelia Acha-Sagredo & Michael J. Pitcher & Gianluca Basso & Luigi Laghi & Manuel Rodriguez-Justo & Jo Spencer & Fran, 2022. "A SIMPLI (Single-cell Identification from MultiPLexed Images) approach for spatially-resolved tissue phenotyping at single-cell resolution," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    12. Mei Lin & Xiao-Long Zhang & Rui You & You-Ping Liu & Hong-Min Cai & Li-Zhi Liu & Xue-Fei Liu & Xiong Zou & Yu-Long Xie & Ru-Hai Zou & Yi-Nuan Zhang & Rui Sun & Wei-Yi Feng & Hai-Yan Wang & Gui-Hua Tao, 2023. "Evolutionary route of nasopharyngeal carcinoma metastasis and its clinical significance," Nature Communications, Nature, vol. 14(1), pages 1-21, December.
    13. Silvia Tiberti & Carlotta Catozzi & Ottavio Croci & Mattia Ballerini & Danilo Cagnina & Chiara Soriani & Caterina Scirgolea & Zheng Gong & Jiatai He & Angeli D. Macandog & Amir Nabinejad & Carina B. N, 2022. "GZMKhigh CD8+ T effector memory cells are associated with CD15high neutrophil abundance in non-metastatic colorectal tumors and predict poor clinical outcome," Nature Communications, Nature, vol. 13(1), pages 1-20, December.
    14. Saba Ahmadi & Pattara Sukprasert & Rahulsimham Vegesna & Sanju Sinha & Fiorella Schischlik & Natalie Artzi & Samir Khuller & Alejandro A. Schäffer & Eytan Ruppin, 2022. "The landscape of receptor-mediated precision cancer combination therapy via a single-cell perspective," Nature Communications, Nature, vol. 13(1), pages 1-17, December.
    15. G. Gambardella & G. Viscido & B. Tumaini & A. Isacchi & R. Bosotti & D. di Bernardo, 2022. "A single-cell analysis of breast cancer cell lines to study tumour heterogeneity and drug response," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    16. Sandra Tietscher & Johanna Wagner & Tobias Anzeneder & Claus Langwieder & Martin Rees & Bettina Sobottka & Natalie Souza & Bernd Bodenmiller, 2023. "A comprehensive single-cell map of T cell exhaustion-associated immune environments in human breast cancer," Nature Communications, Nature, vol. 14(1), pages 1-20, December.
    17. Yiming Lu & Aiqing Yang & Cheng Quan & Yingwei Pan & Haoyun Zhang & Yuanfeng Li & Chengming Gao & Hao Lu & Xueting Wang & Pengbo Cao & Hongxia Chen & Shichun Lu & Gangqiao Zhou, 2022. "A single-cell atlas of the multicellular ecosystem of primary and metastatic hepatocellular carcinoma," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    18. Wanying Wu & Jinyang Zhang & Xiaofei Cao & Zhengyi Cai & Fangqing Zhao, 2022. "Exploring the cellular landscape of circular RNAs using full-length single-cell RNA sequencing," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    19. Jingjing Qi & Hongxiang Sun & Yao Zhang & Zhengting Wang & Zhenzhen Xun & Ziyi Li & Xinyu Ding & Rujuan Bao & Liwen Hong & Wenqing Jia & Fei Fang & Hongzhi Liu & Lei Chen & Jie Zhong & Duowu Zou & Lia, 2022. "Single-cell and spatial analysis reveal interaction of FAP+ fibroblasts and SPP1+ macrophages in colorectal cancer," Nature Communications, Nature, vol. 13(1), pages 1-20, December.
    20. Brian D. Lehmann & Antonio Colaprico & Tiago C. Silva & Jianjiao Chen & Hanbing An & Yuguang Ban & Hanchen Huang & Lily Wang & Jamaal L. James & Justin M. Balko & Paula I. Gonzalez-Ericsson & Melinda , 2021. "Multi-omics analysis identifies therapeutic vulnerabilities in triple-negative breast cancer subtypes," Nature Communications, Nature, vol. 12(1), pages 1-18, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34581-2. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.